Introduction to electron microscopy for biologists

著者

• Allen, Terence D.

書誌事項

Introduction to electron microscopy for biologists

edited by Terence D. Allen

（Methods in cell biology / edited by David M. Prescott, v.88）

Academic Press, 2008

内容説明・目次

内容説明

This volume demonstrates how cellular and associated electron microscopy contributes to knowledge about biological structural information, primarily at the nanometer level. It presents how EM approaches complement both conventional structural biology (at the high end, angstrom level of resolution) and digital light microscopy (at the low end, 100-200 nanometers).

目次

• Scanning Electron Microscopy at the sub-cellular level
• Electron Probe X-ray microanalysis for the study of cell physiology
• Preparation of cells and tissues for Immuno EM
• Fixation, Freezing and Embedding for Genetically Altered Tissue
• Combined video fluorescence and 3-D electron microscopy
• From Live-cell Imaging to Scanning Electron Microscopy (SEM)
• Immunolabelling for High Resolution
• Immunogoldlabeling of Thawed Cryosections
• Close-to-Native Ultrastructural Preservation by High Pressure Freezing
• High-Pressure Freezing and Low-Temperature Fixation of Cell Monolayers Grown on Sapphire Coverslips
• Freeze-fracture Cytochemistry in Cell Biology
• The Plasma membrane cytoskeleton
• Correlated Light and Electron Microscopy of the Cytoskeleton
• Electron microscopy of intermediate filaments
• Studying Microtubules by Electron Microscopy
• Endoplasmic reticulum and Golgi Complex
• Visualization of Dynamins
• Electron Microscopy of Collagen Fibril Structure In Vitro And In Vivo including Three-Dimensional Reconstruction
• Visualisation of Desmosomes in the Electron Microscope
• A Protocol for Isolation and Visualization of Yeast Nuclei by Scanning Electron Microscopy (SEM)
• High Pressure Freezing and Freeze Substitution of S.pombe and S.cerevisiae for TEM
• Electron Microscopy of Lamin and the Nuclear Lamina in Caenorhabditis elegans
• Visualization of Nuclear Organisation by Ultrastructural Cytochemistry
• Scanning Electron Microscopy of Chromosomes
• Infection at the Cellular Level
• Electron Microscopy of Viruses and Virus-Cell Interactions.

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詳細情報

• NII書誌ID(NCID): BA86490097
• ISBN:
  • 9780123743206
• 出版国コード: us
• タイトル言語コード: eng
• 本文言語コード: eng
• 出版地: San Diego,CA
• ページ数/冊数: xv, 545 p., [32] p. of col. plates
• 大きさ: 24 cm
• 親書誌ID:
  • BA00653438