PCR primer design

著者

    • Yuryev, Anton

書誌事項

PCR primer design

edited by Anton Yuryev

(Methods in molecular biology / John M. Walker, series editor, 402)

Humana Press, c2007

大学図書館所蔵 件 / 4

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注記

Includes bibliographical references and index

内容説明・目次

内容説明

At the heart of most high-throughput methods is the technique of polymerase chain reaction (PCR). This book focuses on primer design, which is critical to both the efficiency and the accuracy of the PCR. With intricate descriptions of basic approaches as well as specialized methods, "PCR Primer Design" is an exceptional reference for all those involved in studying the genome.

目次

PCR primer design. Contents Part I. Basic Principles and software for PCR primer design Chapter 1. Physical Principles and Visual-OMP Software for Optimal PCR Design Chapter 2. Oligo 7 Primer Analysis Software Chapter 3. Selection for 3' end triplets for polymerase chain reaction primers Chapter 4. The reference point method in primer design Chapter 5. PCR primer design using statistical modeling Chapter 6. Developing a Statistical Model for Primer Design Part II. Genome scale PCR primer design Chapter 7. GST-PRIME: An Algorithm for Genome-Wide Primer Design Chapter 8. Genome-Scale Probe and Primer Design with PRIMEGENS Repeat masking for PCR primer design Chapter 9. SNPbox: web-based high-throughput primer design with an eye for repetitive sequences. Chapter 10. Fast masking of repeated primer binding sites in eukaryotic genomes Multiplex PCR primer design Chapter 11. Degenerate Primer Design: Theoretical Analysis and the HYDEN program Chapter 12. An Iterative Method for Selecting Degenerate Multiplex PCR Primers Chapter 13. Primer-design for multiplexed genotyping Chapter 14. MultiPLX: automatic grouping and evaluation of PCR primers Chapter 15. MultiPrimer: A system for Microarray PCR Primer Design Allele-specific PCR Chapter 16. Modified oligonucleotides as tools for allele-specific amplification Chapter 17. AlleleID: A Pathogen Detection and Identification System Long PCR primer design Chapter 18. GenoFrag, a software package for the design of primers dedicated to whole genome scanning by LR-PCR. DNA methylation mapping Chapter 19. Designing PCR primer for DNA methylation mapping Chapter 20. BiSearch: ePCR tool for native or bisulfite treated genomic template. Chapter 21. Graphical design of primers with PerlPrimer

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