Fluorescence spectroscopy
著者
書誌事項
Fluorescence spectroscopy
(Methods in enzymology / editors in chief, Sidney P. Colowick, Nathan O. Kaplan, v. 450)
Academic Press, c2008
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注記
Includes bibliographical references and indexes
内容説明・目次
内容説明
Fluorescence spectroscopy is a type of electromagnetic spectroscopy, using a beam of light, which analyzes fluorescence from a sample. Given its extremely high sensitivity and selectivity, it is an important investigational tool in many areas including material sciences, analytical sciences, and across a broad range of chemical, biochemical and medical research. It has become an essential investigational technique allowing detailed, real-time observation of the structure and dynamics of intact biological systems. The pharmaceutical industry uses it heavily and it has become a dominating technique in biochemistry and molecular genetics.
目次
Direct quantitation of Mg2+ - RNA interactions use of a fluorescent dye
Dan Grilley, Ana Maria Soto, and David E. Draper
A Method in Enzymology for Measuring Intracellular Protease Activities in Live Cells
Beverly Z. Packard and Akira Komoriya
Using Fluorophore-labeled Oligonucleotides to Measure Affinities of Protein-DNA Interactions
Brian J. Anderson and Joel F. Schildbach
Site-Selective Red-Edge Effects
Alexander P. Demchenko
Multiparametric Probing of Microenvironment with Solvatochromic Fluorescent Dyes Andrey S. Klymchenko and Alexander Demchenko
Pteridine Probes for Nucleic Acid Analysis
Mary E. Hawkins
Application of Single Molecule Spectroscopy in Studying Enzyme Kinetics and Mechanism Jue Shi, Ari Gafni, and Duncan Steel
Fluorescence Approaches in Quantifying Bio-Molecular Interactions
Catherine A. Royer and Suzanne
Applications of Up-Conversion Methods in Studies of Proteins and Peptides
Jay Knutson
Spectroscopic Studies of Molecular Solvent Relaxation
Mark Maroncelli
Single Molecule Fluorescence Studies of the Dynamics of Gene Regulation
Jie Xiao
New Fluorescence Methods for Studies of Muscle Proteins
Robert E. Dale
Fluorescence Probes for Analysis of Membrane Proteins
Amitabha Chattopadhyay
Fluorescence Methods for Protein-Protein interaction in Membranes
Kalina Hristova
Fluorescence Detection of Specific Ions
Bernard Valeur
Measuring Picomolar Intracellular Exchangable Zn in PC-12 Cells using a Ratiometric Fluorescence Biosensor
R.A. Bozym
Monitoring Free Zinc in the Mitochondrion
Richard B Thompson, et al
Metal-enhanced fluorescence: An emerging tool in biotechnology
Chris D. Geddes, et al
Radiative Decay Engineering, Surface Plasmon-Coupled Directional Emission
Joseph R Lakowicz, et al
Time-resolved fluorescence anisotropy and the measurement of protein conformational flexibility
David A. Johnson (University of California Riverside)
Tranes and other new Analysis Procedures in wavelength resolved fluorescence.
Pariasamy (Mumbay)
From Quantum Theory to Molecular Fluorescence.
D. Toptygin
Probing Protein Electrostatics with Synthetic amino acids.
Bruce Cohen (UCSF)
Analysis of Complex Fluorescence Anisotropy Decay
William Laws, Montana
Studies of Segmental Protein Dynamics with Time and Energy ? Resolved Fluorescence Spectroscopy
D. Toptygin and L. Brand
Complex Fluorescence Decay of Trytophan in Proteins.
Mary D. Barkley
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