Placenta and trophoblast : methods and protocols

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書誌事項

Placenta and trophoblast : methods and protocols

edited by Michael J. Soares and Joan S. Hunt

（Methods in molecular medicine / John M. Walker, series editor, 121-122）

Humana Press, c2006

  • v. 1
  • v. 2

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注記

Includes bibliographical references and indexes

System requirements for accompanying CD-ROM: compatible with Windows and Apple Macintosh

内容説明・目次

巻冊次

v. 1 ISBN 9781588294043

内容説明

A collection of cutting-edge laboratory techniques for the study of trophoblast and placental biology. The techniques presented range from experimental animal models, to animal and human placental organ and cell culture systems, to morphological, biochemical, and molecular strategies for assessing trophoblast/placental growth, differentiation and function. Volume 1 provides readily reproducible protocols for studying embryo-uterine implantation, trophoblast cell development, and the organization and molecular characterization of the placenta. Highlights include strategies for the isolation and culture of trophoblast cells from primates, ruminants, and rodents, and precise guidance to the molecular and cellular analysis of the placental phenotype. A companion second volume concentrates on methods for investigating placental function.

目次

Part I. Introduction Placenta and Trophoblast: Methods and Protocols: Overview I Michael J. Soares and Joan S. Hunt Part II. Methods for Studying Embryo Implantation and Uterine Biology Methodologies to Study Implantation in Mice Kaushik Deb, Jeff Reese, and Bibhash C. Paria Blastocyst Culture D. Randall Armant Isolation of Hormone Responsive Uterine Stromal Cells: An In Vitro Model for Stromal Cell Proliferation and Differentiation Virginia Rider Rat Decidual Cell Cultures Yan Gu and Geula Gibori The Immortalization of Human Endometrial Cells Graciela Krikun, Gil Mor, and Charles Lockwood Sheep Uterine Gland Knockout (UGKO) Model Thomas E. Spencer and C. Allison Gray A Baboon Model for Inducing Endometriosis Asgerally T. Fazleabas A Baboon Model for Simulating Pregnancy Asgerally T. Fazleabas The Common Marmoset Monkey as a Model for Implantation and Early Pregnancy Research Almuth Einspanier, Kai Lieder, Ralf Einspanier, and Bettina Husen Part III. In Vitro Trophoblast and Placental Model Systems Mouse Trophoblast Stem Cells Jennifer Quinn, Tilo Kunath, and Janet Rossant Connexins and Trophoblast Cell Lineage Development Mark Kibschull and Elke Winterhager Rcho-1 Trophoblast Stem Cells: A Model System for Studying Trophoblast Cell Differentiation Namita Sahgal, Lindsey N. Canham, Brent Canham, and Michael J. Soares Bovine Trophoblast Cell Culture Systems: A Technique to Culture Bovine Trophoblast Cells Without Feeder Cells Kazuyoshi Hashizume, Arata Shimada, Haruo Nakano, and Toru Takahashi In Vitro Induction of Trophoblast from Human Embryonic Stem Cells Ren-He Xu Isolation and Culture of Term Human Trophoblast Cells Margaret G. Petroff, Teresa A. Phillips, Hakhyun Ka, Judith L. Pace, andJoan S. Hunt Production of Human Trophoblast Cell Lines Guy St J. Whitley Culture and Transfection of Human Choriocarcinoma Cells Michael W. Wolfe In Vitro Methods for Studying Vascularization of the Murine Allantois and Allantoic Union with the Chorion Karen M. Downs Part IV. Phenotypic Analysis of the Placenta Phenotypic Analysis of the Mouse Placenta David R. C. Natale, Maja Starovic, and James C. Cross Phenotypic Analysis of the Rat Placenta Rupasri Ain, Toshihiro Konno, Lindsey N. Canham, and Michael J. Soares Analysis of the Structure of the Ruminant Placenta: Methods of Fixation, Embedding, and Antibody Localization at Light and Electron Microscope Levels F. B. P. Wooding Characterization of the Bovine Placenta by Cytoskeleton, Integrin Receptors, and Extracellular Matrix Christiane D. Pfarrer Molecular Markers for Human Placental Investigation Berthold Huppertz Correlative Microscopy of Ultrathin Cryosections in Placental Research Toshihiro Takizawa and John M. Robinson Vascular Corrosion Casting of the Uteroplacental and Fetoplacental Vasculature in Mice Kathie J. Whiteley, Christiane D. Pfarrer, and S. Lee Adamson Analysis of Fetal and Maternal Microvasculature in Ruminant Placentomes by Corrosion Casting Rudolf Leiser and Christiane D. Pfarrer Part V. Molecular Analysis and Gene Transfer Techniques Microarray Analysis of Trophoblast Cells Vikram Budhraja and Yoel Sadovsky Gene Expression Microarray Data Analysis of Decidual and Placental Cell Differentiation Sue Kong, Bruce J. Aronow, and Stuart Handwerger Assays to Determine Allelic Usage of Gene Expression in the Placenta Paul B. Vrana Adenoviral-Mediated Gene Delivery to Trophoblast Cells Bing Jiang and Carole R. Mendelson Index
巻冊次

v. 2 ISBN 9781588296085

内容説明

The aim of the two-volume set of Placenta and Trophoblast: Methods and Protocols is to offer contemporary approaches for studying the biology of the placenta. The chapters contained herein also address critical features of the female organ within which the embryo is housed, the uterus, and some aspects of the embryo-fetus itself, particularly those of common experimental animal models. In keeping with the organization used effectively in other volumes in this series, each chapter has a brief introduction followed by a list of required items, protocols, and notes designed to help the reader perform the expe- ments without difficulty. In both volumes, sources of supplies are given and illustrations highlight particular techniques as well as expected outcomes. A key aspect of these volumes is that the contributors are at the forefronts of their disciplines, thus ensuring the accuracy and usefulness of the chapters. Placenta research has progressed rapidly over the past several decades by taking advantage of the technical advances made in other fields. For example, the reader will note that many techniques, such as reverse transcriptase po- merase chain reaction, northern and western blotting, microarray analyses and in situ hybridization experiments, are routinely used for dissecting a wide range of experimental questions. Protein analysis and functional experiments on tissues and cells that comprise the maternal-fetal interface benefit from studies in en- crinology, immunology, and developmental biology. These volumes also present new ideas on investigating gene imprinting and gene transfer via viral vectors.

目次

Part I. Introduction Placenta and Trophoblast: Methods and Protocols: Overview II Michael J. Soares and Joan S. Hunt Part II. Analysis of Trophoblast Invasion In Vivo Analysis of Trophoblast Cell Invasion in the Human Robert Pijnenborg, Elizabeth Ball, Judith N. Bulmer, Myriam Hanssens, Stephen C. Robson, and Lisbeth Vercruysse In Vitro Analysis of Trophoblast Invasion John D. Aplin An In Vitro Model of Trophoblast Invasion of Spiral Arteries Judith E. Cartwright and Mark Wareing Part III. Analysis of Uteroplacental Immune Cells and Their Functions In Vivo Models for Studying Homing and Function of Murine Uterine Natural Killer Cells B. Anne Croy and Xuemei Xie Immune and Trophoblast Cells at the Rhesus Monkey Maternal-Fetal Interface Thaddeus G. Golos, Gennadiy I. Bondarenko, Edith E. Breburda, Svetlana V. Dambaeva, Maureen Durning, and Igor I. Slukvin Methods for Isolation of Cells from the Human Fetal-Maternal Interface Anita Trundley, Lucy Gardner, Jacquie Northfield, Chiwen Chang, and Ashley Moffett In Vitro Models for Studying Human Uterine and Placental Macrophages Ramsey H. McIntire, Margaret G. Petroff, Teresa A. Phillips, and Joan S. Hunt Macrophage-Trophoblast Interactions Gil Mor, Shawn L. Straszewski-Chavez, and Vikki M. Abrahams Methods for Evaluating Histocompatibility Antigen Gene Expression in the Baboon Daudi K. Langat, Asgerally T. Fazleabas, and Joan S. Hunt Analysis of the Soluble Isoforms of HLA-G: mRNAs and Proteins Judith L. Pace, Pedro J. Morales, Teresa A. Phillips, and Joan S. Hunt Part IV. Analysis of Placental Function: Transport and Endocrinology In Vivo Techniques for Studying Fetoplacental Nutrient Uptake, Metabolism, and Transport Timothy R. H. Regnault and William W. Hay, Jr. In Vitro Models for Studying Trophoblast Transcellular Transport Claudia J. Bode, Hong Jin, Erik Rytting, Peter S. Silverstein, Amber M. Young, and Kenneth L. Audus In Vitro Methods for Studying Human Placental Amino Acid Transport: Placental Plasma Membrane Vesicles Jocelyn D. Glazier and Colin P. Sibley In Vitro Methods for Studying Human Placental Amino Acid Transport: Placental Villous Fragments Susan L. Greenwood and Colin P. Sibley Methods for Investigating Placental Fatty Acid Transport Yan Xu, Thomas J. Cook, and Gregory T. Knipp Heterologous Expression Systems for Studying Placental Transporters Vadivel Ganapathy, You-Jun Fei, and Puttur D. Prasad Analysis of Trophoblast Giant Cell Steroidogenesis in Primary Cultures Noa Sher and Joseph Orly Establishment of an ELISA for the Detection of Native Bovine Pregnancy-Associated Glycoproteins Secreted by Trophoblast Binucleate Cells Jonathan A. Green and R. Michael Roberts Alkaline Phosphatase Fusion Proteins as Tags for Identifying Targets for Placental Ligands Heiner Muller and Michael J. Soares Bacterial Expression of Prolactin Family Proteins Arieh Gertler Analysis of Placental Regulation of Hematopoiesis Beiyan Zhou and Daniel I. H. Linzer Methods for Studying Interferon Tau Stimulated Genes Fuller W. Bazer and Thomas E. Spencer Part V. Analysis of Placenta Adaptation to Disease Reduced Uterine Perfusion Pressure (RUPP) Model for Studying Cardiovascular-Renal Dysfunction in Response to Placental Ischemia Joey P. Granger, B. Babbette D. LaMarca, Kathy Cockrell, Mona Sedeek, Charles Balzi, Derrick Chandler, and William Bennett In Vivo Rat Model of Preeclampsia S. Ananth Karumanchi and Isaac E. Stillman A Novel Mouse Model for Preeclampsia by Transferring Activated Th1 Cells into Normal Pregnant Mice Ana Claudia

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