Adenoviruses, ad vectors, quantitation, and animal models

著者

書誌事項

Adenoviruses, ad vectors, quantitation, and animal models

edited by William S.M. Wold, Ann E. Tollefson

（Methods in molecular medicine / John M. Walker, series editor, . Adenovirus methods and protocols ; vol.1）

Humana Press, c2007

2nd ed

  • : [hbk.]
  • : [ebook]

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注記

Includes bibliographical references and index

内容説明・目次

巻冊次

: [hbk.] ISBN 9781588295989

内容説明

Adenovirus Methods and Protocols, Second Edition, now in two volumes, is an essential resource for adenovirus (Ad) researchers beginning in the field, and an inspirational starting point for researchers looking to branch into new areas of Ad study. In addition to updating and expanding the first edition, the authors have added new chapters that address innovative areas of emphasis in Ad research, including Ad vector construction and use, real-time PCR, use of new animal models, and methods for quantification of Ad virus or virus expression/interactions. Each of the protocols presented in these volumes is written by trendsetting researchers.

目次

TABLE OF CONTENTS Vol. I Topics: Adenoviruses and vectors, quantitation, and animal models 1. Manipulation of Early Region 4 Julie Boyer and Gary Ketner 2. Isolation, Growth, and Purification of Defective Adenovirus Deletion Mutants Gary Ketner and Julie Boyer 3. Construction of Adenovirus Type 5 Early Region 1 (E1) and 4 (E4) Virus Mutants Peter Groitl and Thomas Dobner 4. Construction of Mouse Adenovirus Type 1 Mutants Angela N. Cauthen, Amanda R. Welton, and Katherine R. Spindler 5. Generation of Recombinant Adenovirus using E.coli BJ5183 Recombination System P. Seshidhar Reddy, Shanthi Ganesh, Lynda Hawkins, Neeraja Idamakanti 6. Production and Release Testing of Ovine AtadenovirusVectors Gerald W. Both, Fiona Cameron, Anne Collins, Linda J. Lockett, Jan Shaw 7. Construction of Capsid Modified Recombinant Bovine Adenovirus Type 3 Alexander N. Zakhartchouk, Qiaohua Wu, Suresh K. Tikoo 8. Adenovirus Capsid Chimeras: Fiber Terminal Exon Insertions / Gene Replacements in the MLTU Jason Gall, John Schoggins, and Erik Falck-Pedersen 9. Temperature-Sensitive Replication-Competent Adenovirus shRNA Vectors to Study Cellular Genes in Virus-Induced Apoptosis T. Subramanian and G. Chinnadurai 10. Evaluating Apoptosis in Tumor Cells Infected with Adenovirus Expressing p53 Tumor Suppressor Gene Elizabeth Perron, M. Behzad Zafar, Amanda Pister, Zhen-Guo Wang, Jun Tian and Prem Seth 11. Growth and Purification of Enteric Adenovirus Type 40 Vivien Mautner 12. Immunocompetent, Semi-permissive Cotton Rat Tumor Model for the Evaluation of Oncolytic Adenoviruses Karoly Toth, Jacqueline F. Spencer, William S.M. Wold 13. The Use of the Syrian Hamster as an Animal Model for Oncolytic Adenovirus Vectors Maria A. Thomas, Jacqueline F. Spencer, and William S. M. Wold 14. A Real-Time PCR Method to Rapidly Titer Adenovirus Stocks Maria A. Thomas, Drew L. Lichtenstein, Peter Krajcsi, and William S. M. Wold 15. Detection and Quantitation of Subgroup C Adenovirus DNA in Human Tissue Samples by Real-Time PCR C.T. Garnett, Ching-I Pao, Linda R. Gooding 16. Flow Cytometric Detection of Adenoviruses and Intracellular Adenovirus Proteins Graham Bottley, John R. Holt, Nicola J. James and G. Eric Blair 17. Capture ELISA Quantitation of Mouse Adenovirus Type 1 in Infected Organs Amanda R. Welton and Katherine R. Spindler 18. Preparation and Titration of CsCl-Banded Adenovirus Stocks Ann E. Tollefson, Mohan Kuppuswamy, Elena V. Shashkova, Konstantin Doronin, and William S. M. Wold
巻冊次

: [ebook] ISBN 9781597451666

内容説明

"Adenovirus Methods and Protocols" is designed to help new researchers to conduct studies involving adenoviruses and to help established researchers to branch into new areas. The initial chapters of "Adenovirus Methods and Protocols, Volume 1" address several techniques for construction of adenoviruses for use as vectors and for basic research. The topics include deletion mutants, capsid modifications, insertions, and gene replacements both in human adenoviruses and murine, bovine, and ovine adenoviruses.

目次

  • TABLE OF CONTENTS
  • Vol. I Topics: Adenoviruses and vectors, quantitation, and animal models
  • 1. Manipulation of Early Region 4
  • Julie Boyer and Gary Ketner
  • 2. Isolation, Growth, and Purification of Defective Adenovirus Deletion Mutants
  • Gary Ketner and Julie Boyer
  • 3. Construction of Adenovirus Type 5 Early Region 1 (E1) and 4 (E4) Virus Mutants
  • Peter Groitl and Thomas Dobner
  • 4. Construction of Mouse Adenovirus Type 1 Mutants
  • Angela N. Cauthen, Amanda R. Welton, and Katherine R. Spindler
  • 5. Generation of Recombinant Adenovirus using E.coli BJ5183 Recombination System
  • P. Seshidhar Reddy, Shanthi Ganesh, Lynda Hawkins, Neeraja Idamakanti
  • 6. Production and Release Testing of Ovine AtadenovirusVectors
  • Gerald W. Both, Fiona Cameron, Anne Collins, Linda J. Lockett, Jan Shaw
  • 7. Construction of Capsid Modified Recombinant Bovine Adenovirus Type 3
  • Alexander N. Zakhartchouk, Qiaohua Wu, Suresh K. Tikoo
  • 8. Adenovirus Capsid Chimeras: Fiber Terminal Exon Insertions / Gene Replacements in the MLTU
  • Jason Gall, John Schoggins, and Erik Falck-Pedersen
  • 9. Temperature-Sensitive Replication-Competent Adenovirus shRNA Vectors to Study Cellular Genes in Virus-Induced Apoptosis
  • T. Subramanian and G. Chinnadurai
  • 10. Evaluating Apoptosis in Tumor Cells Infected with Adenovirus Expressing p53 Tumor Suppressor Gene
  • Elizabeth Perron, M. Behzad Zafar, Amanda Pister, Zhen-Guo Wang, Jun Tian and Prem Seth
  • 11. Growth and Purification of Enteric Adenovirus Type 40
  • Vivien Mautner
  • 12. Immunocompetent, Semi-permissive Cotton Rat Tumor Model for the Evaluation of Oncolytic Adenoviruses
  • Karoly Toth, Jacqueline F. Spencer, William S.M. Wold
  • 13. The Use of the Syrian Hamster as an Animal Model for Oncolytic Adenovirus Vectors
  • Maria A. Thomas, Jacqueline F. Spencer, and William S. M. Wold
  • 14. A Real-Time PCR Method to Rapidly Titer Adenovirus Stocks
  • Maria A. Thomas, Drew L. Lichtenstein, Peter Krajcsi, and William S. M. Wold
  • 15. Detection and Quantitation of Subgroup C Adenovirus DNA in Human Tissue Samples by Real-Time PCR
  • C.T. Garnett, Ching-I Pao, Linda R. Gooding
  • 16. Flow Cytometric Detection of Adenoviruses and Intracellular Adenovirus Proteins
  • Graham Bottley, John R. Holt, Nicola J. James and G. Eric Blair
  • 17. Capture ELISA Quantitation of Mouse Adenovirus Type 1 in Infected Organs
  • Amanda R. Welton and Katherine R. Spindler
  • 18. Preparation and Titration of CsCI-Banded Adenovirus Stocks.

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