Ad proteins, RNA, lifecycle, host interactions, and phylogenetics

著者

書誌事項

Ad proteins, RNA, lifecycle, host interactions, and phylogenetics

edited by William S.M. Wold, Ann E. Tollefson

（Methods in molecular medicine / John M. Walker, series editor, . Adenovirus methods and protocols ; vol.2）

Humana Press, c2007

2nd ed

  • : [hbk.]
  • : [ebook]

大学図書館所蔵 件 / 全1件

この図書・雑誌をさがす

注記

Includes bibliographical references and index

内容説明・目次

巻冊次

: [hbk.] ISBN 9781588299017

内容説明

Adenovirus Methods and Protocols, Second Edition, now in two volumes, is an essential resource for adenovirus (Ad) researchers beginning in the field, and an inspirational starting point for researchers looking to branch into new areas of Ad study. In addition to updating and expanding important chapters from the first edition, the authors have added new chapters that address innovative, exciting areas of emphasis in Ad research, including Ad vector construction and use, real-time PCR, use of new animal models, and methods for quantification of Ad virus or virus expression/interactions. The protocols presented are written by trendsetting researchers.

目次

Ann E. Tollefson, Mohan Kuppuswamy, and William S. M. Wold TABLE OF CONTENTS Vol. II Topics: Ad proteins and RNA, lifecycle and host interactions, and phylogenetics 1. Analysis of the Efficiency of Adenovirus Transcription Cristina Iftode and S. J. Flint 2. The Use of In Vitro Transcription to Probe Regulatory Functions of Viral Protein Domains Paul M. Loewenstein, Chao-Zhong Song and Maurice Green 3. Preparation of Soluble Extracts from Adenovirus Infected Cells for Studies of RNA Splicing Oliver Muhlemann and Goeran Akusjarvi 4. In Vitro Methods to Study RNA Interference During an Adenovirus Infection Gunnar Andersson, Ning Xu and Goeran Akusjarvi 5. Simultaneous Detection of Adenovirus RNA and Cellular Proteins by Fluorescent Labeling In Situ Eileen Bridge 6. Study of Nucleolar Localization of Adenovirus Core Proteins David A. Matthews 7. Analysis of Adenovirus Infections in Synchronized Cells David A. Ornelles, Robin N. Shepard and Felicia D. Goodrum 8. Co-Immunoprecipitation of Protein Complexes Peter Yaciuk 9. Chromatin Immunoprecipitation (ChIP) to Study the Binding of Proteins to the Adenovirus Genome in Vivo Jihong Yang and Patrick Hearing 10. Assaying Protein-DNA Interactions In Vivo and In Vitro Using Chromatin Immunoprecipitation and Electrophoretic Mobility Shift Assays Pilar Perez-Romero and Michael J. Imperiale 11. Identifying Functional Adenovirus-Host Interactions Using Tandem Mass Spectrometry Anuj Gaggar, Dmitry Shayakhmetov, and Andre Lieber 12. The Use of Cell Microinjection for the in vivo Analysis of Viral Transcriptional Regulatory Protein Domains Maurice Green, Andrew Thorburn, Robert Kern and Paul M. Loewenstein 13. Determination of the Transforming Activities of Adenovirus Oncogenes Michael Nevels and Thomas Dobner 14. Human Adenovirus Type 12: Crossing Species Barriers toImmortalize the Viral Genome Walter Doerfler 15. Measurement of NK Cell Lytic Activity of Adenovirus-Infected or Adenovirus-Transformed Cells John M. Routes 16. A Flow Cytometric Assay for Analysis of NK Cell-Mediated Cytolysis of Adenovirus-Transformed Cells Graham Bottley, Graham P. Cook, and G. Eric Blair 17. Large Scale Purification and Crystallization of Adenovirus Hexon John J. Rux and Roger M. Burnett 18. Synthesis and Assay of Recombinant Adenovirus Protease Joseph M. Weber 19. Assay for the Adenovirus Proteinase- Purification of the Enzyme and Synthesis of a Fluorogenic Substrate Walter F. Mangel and William J. McGrath 20. Cofactors of the Adenovirus Proteinase: Measuring Equilibrium Dissociation Constants and Stoichiometries of Binding Walter F. Mangel and William J. McGrath 21. Characterization of the Adenovirus Fiber Protein Jeffrey A. Engler and Jeong Shin Hong 22. Phylogenetic Analysis of Adenovirus Sequences Balazs Harrach and Maria Benkoe 23. Assessment of Genetic Variability Among Subspecies B1 Human Adenoviruses for Molecular Epidemiology Studies Adriana E. Kajon and Dean D. Erdman
巻冊次

: [ebook] ISBN 9781597452779

内容説明

"Adenovirus Methods and Protocols" is designed to help new researchers to conduct studies involving adenoviruses and to help established researchers to branch into new areas. "Adenovirus Methods and Protocols, Volume II" focuses on methods that elucidate and quantitate the interactions of adenoviruses with the host. This volume provides methods for analysis of transcription, splicing, RNA interference, subcellular localization of proteins during infection, and cell cycle effects.

目次

  • Ann E. Tollefson, Mohan Kuppuswamy, and William S. M. Wold
  • TABLE OF CONTENTS
  • Vol. II Topics: Ad proteins and RNA, lifecycle and host interactions, and phylogenetics
  • 1. Analysis of the Efficiency of Adenovirus Transcription
  • Cristina Iftode and S. J. Flint
  • 2. The Use of In Vitro Transcription to Probe Regulatory Functions of Viral Protein Domains
  • Paul M. Loewenstein, Chao-Zhong Song and Maurice Green
  • 3. Preparation of Soluble Extracts from Adenovirus Infected Cells for Studies of RNA Splicing
  • Oliver Muhlemann and Goran Akusjarvi
  • 4. In Vitro Methods to Study RNA Interference During an Adenovirus Infection
  • Gunnar Andersson, Ning Xu and Goran Akusjarvi
  • 5. Simultaneous Detection of Adenovirus RNA and Cellular Proteins by Fluorescent Labeling In Situ
  • Eileen Bridge
  • 6. Study of Nucleolar Localization of Adenovirus Core Proteins
  • David A. Matthews
  • 7. Analysis of Adenovirus Infections in Synchronized Cells
  • David A. Ornelles, Robin N. Shepard and Felicia D. Goodrum
  • 8. Co-Immunoprecipitation of Protein Complexes
  • Peter Yaciuk
  • 9. Chromatin Immunoprecipitation (ChIP) to Study the Binding of Proteins to the Adenovirus Genome in Vivo
  • Jihong Yang and Patrick Hearing
  • 10. Assaying Protein-DNA Interactions In Vivo and In Vitro Using Chromatin Immunoprecipitation and Electrophoretic Mobility Shift Assays
  • Pilar Perez-Romero and Michael J. Imperiale
  • 11. Identifying Functional Adenovirus-Host Interactions Using Tandem Mass Spectrometry
  • Anuj Gaggar, Dmitry Shayakhmetov, and Andre Lieber
  • 12. The Use of Cell Microinjection for the in vivo Analysis of Viral Transcriptional Regulatory Protein Domains
  • Maurice Green, Andrew Thorburn, Robert Kern and Paul M. Loewenstein
  • 13. Determination of the Transforming Activities of Adenovirus Oncogenes
  • Michael Nevels and Thomas Dobner
  • 14. Human Adenovirus Type 12: Crossing Species Barriers to Immortalize the Viral Genome
  • Walter Doerfler
  • 15. Measurement of NK Cell Lytic Activity of Adenovirus-Infected or Adenovirus-Transformed Cells
  • John M. Routes
  • 16. A Flow Cytometric Assay for Analysis of NK Cell-Mediated Cytolysis of Adenovirus-Transformed Cells
  • Graham Bottley, Graham P. Cook, and G. Eric Blair
  • 17. Large Scale Purification and Crystallization of Adenovirus Hexon
  • John J. Rux and Roger M. Burnett
  • 18. Synthesis and Assay of Recombinant Adenovirus Protease
  • Joseph M. Weber
  • 19. Assay for the Adenovirus Proteinase- Purification of the Enzyme and Synthesis of a Fluorogenic Substrate
  • Walter F. Mangel and William J. McGrath
  • 20. Cofactors of the Adenovirus Proteinase: Measuring Equilibrium Dissociation Constants and Stoichiometries of Binding
  • Walter F. Mangel and William J. McGrath
  • 21. Characterization of the Adenovirus Fiber Protein
  • Jeffrey A. Engler and Jeong Shin Hong
  • 22. Phylogenetic Analysis of Adenovirus Sequences
  • Balazs Harrach and Maria Benko
  • 23. Assessment of Genetic Variability Among Subspecies B1 Human Adenoviruses for Molecular Epidemiology Studies
  • Adriana E. Kajon and Dean D. Erdman.

「Nielsen BookData」 より

関連文献： 1件中  1-1を表示

詳細情報

ページトップへ