Microfluidic applications in biology : from technologies to systems biology
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書誌事項
Microfluidic applications in biology : from technologies to systems biology
Wiley-VCH, c2006
- : hbk.
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
Taken from the high-impact journal Electrophoresis, these research articles on microfluidics and its application in a range of biological fields are of high interest and now available to a new readership. Alongside several review articles, this volume represents a current overview of the latest research.
目次
Chapter 1.On-line chemiluminescence detection for isoelectric focusing of heme proteins on microchips. Chapter 2. A simple microfluidic system for efficient capillary electrophoretic separation and sensitive fluorimetric detection of DNA fragments using light-emitting diode and liquid-core waveguide techniques. Chapter 3. Determination ob biochemical species on electrophoresis chips with an external contactless conductivity detector. Chapter 4. In-channel indirect amperometric detection of nonelectroactive anions for electrophoresis on a poly(dimethylsiloxane) microchip. Chapter 5. Coupling on-chip solid-phase extraction to electrospray mass spectrometry through an integrated electrospray tip. Chapter 6. Electrospray interfacing of polymer microfluidics to MALDI-MS. Chapter 7. Nanoliquid chromatography-mass spectrometry of oligosaccharides employing graphitized carbon chromatography on microchip with a high-accuracy mass analyzer. Chapter 8. Chip electrospray mass spectrometry for carbohydrate analysis. Chapter 9. Utility of lab-on-a-chip technology for high-throughput nucleic acid and protein analysis. Chapter 10. Analysis of amino acids and proteins using a poly (methyl methacrylate) microfluidic system. Chapter 11. Single cell manipulation, analytics, and label-free protein detection microfluidic devices for systems nanobiology. Chapter 12. Fast immobilization of probe beads by dielectrophoresis-controlled adhesion in a versatile microfluidic platform for affinity assay. Chapter 13. Droplet fusion by alternating current (AC) field electrocoalescence in microchannels. Chapter 14. Microfluidic flow focusing: Drop size and scaling in pressure versus flow-rate-driven pumping. Chapter 15. Aligning fast alternating current electroosmotic flow fields and characteristic frequencies with dielectrophoretic traps to achieve rapid bacteria detection. Chapter 16. Dielectrophoresis induced clustering regimes of viable yeast cells. Chapter 17. 3-D electrode designs for flow-through dielectrophoretic systems. Chapter 18. Parallel mixing of photolithographically defined nanoliter volumes using elastomeric microvalve arrays. Chapter 19. Method development and measurements of endogenous serine/threonine akt phosphorylation using capillary electrophoresis for systems biology. Chapter 20. Comparison of a pump-around, a diffusion-driven, and a shear-driven system for the hybridization of mouse lung and testis total RNA on microarrays. Chapter 21. Microfluidic devices for the analysis of apoptosis. Chapter 22. Effect of iron restriction on outer membrane protein composition of pseudomonas strains studied by conventional and microchip electrophoresis. Index.
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