Molecular biomethods handbook
著者
書誌事項
Molecular biomethods handbook
Humana Press, c2008
2nd ed
- : hbk
- : pbk
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注記
Includes bibliographical references and index
収録内容
- The manipulation of nucleic acids : basic tools and techniques / Gayle Corkill and Ralph Rapley
- Restriction enzymes : tools in clinical research / Gareth J.S. Jenkins
- Principles and medical applications of the polymerase chain reaction / Bimal D.M. Theophilus
- Probe design, production, and applications / Marilena Aquino de Muro
- Southern blotting as a diagnostic method / Bronwen M. Harvey and Pirkko Soundy
- Capillary electrophoresis of DNA : biomedical applications / Beatriz Sanchez-Vega
- Denaturing high-performance liquid chromatography (DHPLC) for nucleic acid analysis / Kim Hung Leung and Shea Ping Yip
- Denaturing gradient gel electrophoresis (DGGE) / Jeroen H. Roelfsema and Dorien J.M. Peters
- Single strand conformation polymorphism (SSCP) analysis / Kim Hung Leung and Shea Ping Yip
- Randomly amplified polymorphic DNA (RAPD) : a useful tool for genomic characterization of different organisms / Lúcia Maria da Cunha Galvão and Eliane Lages-Silva
- Quantification of mRNA using real time RT-PCR : the SYBR solutiion / David Sugden and Patricia de Winter
- Quantitative analysis of DNA sequences by PCR and solid-phase minisequencing / Anu Suomalainen and Ann-Christine Syvänen
- Multiplex amplifiable probe hybridization (MAPH) / Carolina Sismani, Ludmila Kousoulidou, and Philippos C. Patsalis
- Gene expression profiling / Arnis Druka, Robbie Waugh, and Pete Hedley
- Comparative genomic hybridization in clinical and medical research / Peng-Hui Wang, Yann-Jang Chen, and Chi-Hung Lin
- Subtractive hybridization / Craig Winstanley
- Fluorescence in situ hybridization / Jane Bayani and Jeremy A. Squire
- Quantitative trait locus mapping to identify genes for complex traits in mice / Jonathan D. Smith
- cDNA microarrays / Phillip G. Febbo
- Mapping techniques / Simon G. Gregory
- Single nucleotide polymorphisms / Nameeta Shah
- Gene/protein sequence analysis : a compilation of bioinformatic tools / Bernd H.A. Rehm and Frank Reinecke
- Protein electrophoresis / David Sheehan and Siobhan O'Sullivan
- Protein blotting / Patricia Gravel
- Capillary electrophoresis of proteins / Mark Strege
- Autoradiograph and fluorography / Bronwen M. Harvey
- Mass spectrometry of proteins and peptides / Kenneth G. Standing
- Post-translational modifications of proteins / Christoph Kannich and Birte Fuchs
- Protein microarray technology / Charlotte H. Clarke and Eric T. Fung
- Protein-protein interactions / Hae Ryoun Park ... [et al.]
- Glycoprotein analysis / Terry D. Butters and David C.A. Neville
- Solid-phase peptide synthesis / Mare Cudic and Gregg B. Fields
- Monoclonal antibodies / Zhong J. Zhang and Maher Albitar
- Antibody phage display / Rob Aitken
- Protein engineering / Thomas Willemsen ... [et al.]
- Directed protein evolution / Sabine C. Stebel ... [et al.]
- Enzyme linked immunosorbent assay (ELISA) / John Crowther
- Epitope mapping : identification of antibody-binding sites on protein antigens / Glenn E. Morris
- Quantum dots / Charles Z. Hotz
- Ion-exchange chromatography / David Sheehan
- Size-exclusion chromatography / Paul Cutler
- Hydrophobic interaction chromatography / Paul A. O'Farrell
- Affinity chromatography / Adam Charlton and Michael Zachariou
- High performance liquid chromatography (HPLC) of peptides and proteins / Tzong-Hsien Lee and Marie-Isabel Aguilar
- Amino acid analysis / Ian Davidson and Paula O'Connor
- Surface plasmon resonance / K. Scott Phillips and Quan Jason Cheng
- Macromolecular crystallography / Bernhard Rupp and Katherine A. Kantardjieff
- Microchip devices for bioanalysis / Anna C. Kinsella and Shelley D. Minteer
- Mammalian cell culture / Simon P. Langdon
- Plant tissue culture / Victor M. Loyola-Vargas ... [et al.]
- Stem cells and regenerative medicine / Mohan C. Vemuri and Chellu S. Chetty
- Cryopreservatioin : conservation of bioresources at ultra low temperatures / John G. Day ... [et al.]
- Magnetic resonance imaging / Pottumarthi V. Prasad and Pippa Storey
- Electron microscopy / John Kuo
- Confocal microscopy / Guy Cox
- Laser microdissection / Graeme I. Murray
- Biomedical uses of flow cytometry / James L. Weaver and Maryalice Stetler-Stevenson
- Immunomicroscopy / Constance Oliver
- In situ hybridization /Ian A. Darby and Tim D. Hewitson
- High throughput screening / William P. Janzen
内容説明・目次
内容説明
Recent advances in the biosciences have led to a range of powerful new technologies, particularly nucleic acid, protein and cell-based methodologies. The most recent insights have come to affect how scientists investigate and define cellular processes at the molecular level. This book expands upon the techniques included in the first edition, providing theory, outlines of practical procedures, and applications for a range of techniques. Written by a well-established panel of research scientists, the book provides an up-to-date collection of methods used regularly in the authors' own research programs.
目次
- Molecular Biomethods Handbook 2nd Ed Contents PART A: NUCLEIC ACID BIOMETHODS 1 The manipulation of nucleic acids: basic tools and techniques - Gayle Corkill and Ralph Rapley 2 Restriction Enzymes - Gareth J.S. Jenkins 3 Principles and Medical Applications of the Polymerase Chain Reaction - Birmal D.M. Theophilus 4 Probe Design, Production, and Applications - Marilena Aquino de Muro 5 Southern Blotting as a Diagnostic Method - Bronwen Harvey and Pirkko Soundy 6 Introduction to Capillary Electrophoresis of DNA - Beatriz Sanchez-Vega 7 Denaturing High-Performance Liquid Chromatography (DHPLC) for nucleic acid analysis - Kim Hung Leung and Shea Ping Yip 8 Denaturing Gradient Gel Electrophoresis (DGGE) - Jeroen H. Roelfsema and Dorien J.M. Peters 9 Single Strand Conformation Polymorphism (SSCP) Analysis - Kim Hung Leung and Shea Ping Yip 10 Randomly amplified polymorphic DNA (RAPD)
- a useful tool for genomic characterization of different organisms - Lucia Maria da Cunha Galvao and Eliane Lages-Silva 11 Quantification of mRNA using Real Time RT-PCR - the SYBR solution - David Sugden and Patricia de Winter 12 Quantitative Analysis of DNA Sequences by PCR and Solid Phase Sequencing - Anu Suomalainen and Ann-Christine Syvanen 13 MAPH (Multiplex Amplifiable Probe Hybridization) - Carolina Sismani, Ludmila Kousoulidou and Philippos C. Patsalis 14 Gene Expression Profiling - Arnis Druka, Robbie Waugh and Pete Hedley 15 Comparative Genomic Hybridization in Clinical and Medical Research - Peng-Hui Wang, Yann-Jang Chen and Chi-Hung Lin 16Subtractive Hybridization - Craig Winstanley 17 Fluorescence in situ Hybridization - Jane Bayani and Jeremy A. Squire 18 Quantitative Trait Locus Mapping to Identify Genes for Complex Traits in Mice - Jonathan D. Smith 19 cDNA Microarrays - Phillip G. Febbo 20 Mapping Techniques - Simon G. Gregory 21 Single Nucleotide Polymorphisms Overview - Nameeta Shah 22 Bioinformatics Tools for Gene and Protein Sequence Analysis- Bernd H.A. Rehm and Frank Reinecke PART B: PROTEIN AND CELL METHODS 23. Protein Electrophoresis - David Sheehan and Siobhan O'Sullivan 24. Protein blotting - Patricia Gravel 25. Capillary electrophoresis of proteins - Mark Strege 26. Autoradiography and fluorography - Bronwen Harvey 27. Mass spectrometry of proteins & peptides - Kenneth G. Standing 28. Post-translational modifications - Christoph Kannicht and Birte Fuchs 29. Protein microarray technology - Charlotte H. Clarke and Eric T. Fung 30. Protein-protein interactions - Hae Ryoun Park, Lisa Montoya Cockrell, Yuhong Du, Andrea Kasinski, Jonathan Havel, Jing Zhao, Francisca Reyes-Turcu, Keith Wilkinson and Haian Fu 31. Glycoprotein analysis - Terry Butters and David C.A. Neville 32. Solid Phase Peptide Synthesis - Mare Cudic and Gregg B. Fields 33. Monoclonal antibodies - Zhong J. Zhang and Maher Albitar 34. Antibody Phage display - Rob Aitken 35. Protein engineering - Thomas Willemsen, Urs B. Hagemann, Eva M. Jouaux, Sabine Stebel, Jody M. Mason, Kristian M. Muller and Katja M. Arndt 36. Directed protein evolution - Sabine C. Stebel, Annette Gaida, Katja M. Arndt and Kristian M. Muller 37. Enzyme Linked Immunosorbent Assay - John Crowther 38. Epitope mapping - Glenn E. Morris 39.
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