書誌事項

Avian influenza virus

Erica Spackman, editor

(Methods in molecular biology / John M. Walker, series editor, v. 436)

Humana Press, c2008

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注記

Includes bibliographical references and index

内容説明・目次

内容説明

With the growing global fear of a major pandemic, avian influenza (AI) virus research has greatly increased in importance. In Avian Influenza Virus, an expert team of researchers and diagnosticians examine the fundamental, yet essential, virological methods for AI virus research and diagnostics as well as some of the newest molecular procedures currently used for basic and applied research. They present exciting, cutting-edge new methods that focus both on studying the virus itself and on work with avian hosts, an area greatly lacking in research.

目次

Avian Influenza Virus Table of Contents 1. A brief introduction to avian influenza virus Erica Spackman 2. Avian Influenza Virus Sample Types, Collection, and Handling Mary-Lea Killian 3. Avian influenza virus RNA extraction from tissue and swab material Erica Spackman and David L. Suarez 4. Type-A Influenza Virus Detection and Quantitation by Real-time RT-PCR Erica Spackman and David L. Suarez 5. Detection and Identification of the H5 Hemagglutinin subtype by Real-time RT-PCR Erica Spackman and David L. Suarez 6. Avian influenza virus isolation and propagation in chicken eggs Peter R. Woolcock 7. Hemagglutination Assay for Avian Influenza Virus Mary-Lea Killian 8. Hemagglutination-Inhibition Test for Avian Influenza Virus Subtype Identification and the Detection and Quantititation of Serum Antibodies to Avian Influenza Virus Janice C. Pedersen 9. Neuraminidase-Inhibition Assay for the Identification of Influenza A Virus Neuraminidase Subtype or Neuraminidase Antibody Specificity Janice C. Pedersen 10. Immunohistochemical Staining for the Detection of Avian Influenza Virus in Tissues Mary Pantin-Jackwood 11. Wild bird Surveillance for Avian Influenza Virus Justin D. Brown and David E. Stallknecht 12. Reverse Genetics of Avian Influenza Virus Chang-Won Lee and David L. Suarez 13. Evaluating the Cell Mediated Immune Response of Avian Species to Avian Influenza Viruses Darrell R. Kapczynski 14. Measurment of avian cytokines with real-time RT-PCR following infection with avian influenza virus Darrell R. Kapczynski and Michael H. Kogut

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