Cell-based assays for high-throughput screening : methods and protocols
著者
書誌事項
Cell-based assays for high-throughput screening : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, v. 486）
Humana Press, c2009
- : hbk
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注記
Includes index
内容説明・目次
内容説明
As the use of high-throughput screening expands and creates more interest in the academic community, the need for detailed reference materials becomes ever more pressing. Cell-Based Assays for High-Throughput Screening: Methods and Protocols aims to fill an important part of this need by providing an easily accessible reference volume for cell-based phenotypic screening. Leading researchers in the field contribute state-of-the-art methods with actionable protocols covering four major areas of study: model biological systems, screening modalities and assay systems, detection technologies, and approaches to data analysis. Written in the highly successful Methods in Molecular Biology (TM) series format, each chapter includes a brief introduction to the subject, lists of necessary materials and reagents, step-by-step laboratory protocols, and a Notes section detailing tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and easy-to-use, Cell-Based Assays for High-Throughput Screening: Methods and Protocols presents an overview of relevant approaches, enabling the direct application of existing methods to new discoveries while also inspiring researchers to approach their screening projects in a conceptually modular fashion, enhancing the power to discover through new combinations of existing approaches.
目次
Chapter 1: Introduction: Cell-based Assays for High-throughput Screening W. Frank An and Nicola J. Tolliday Chapter 2: High-throughput Screening of Model Bacteria Soumaya Zlitni, Jan E. Blanchard, and Eric D. Brown Chapter 3: Cell-based Assays to Probe the ERK MAP Kinase Pathway in Endothelial Cells Michael R. Wyler, Deborah H. Smith, Eftihia Cayanis, Udo Toebben, Nathalie Aulner, and Thomas Mayer Chapter 4: Large-scale Small-molecule Screen Using Zebrafish Embryos Charles C. Hong Chapter 5: Whole-animal High-throughput Screens: The C. elegans Model Eyleen J. O'Rourke, Annie L. Conery, and Terence I. Moy Chapter 6: Whole-organism Screening: Plants April Agee and David Carter Chapter 7: Fluorescence-based Assays W. Frank An Chapter 8: Reporter-gene Assays Andy M. F. Liu, David C. New, Rico K. H. Lo, and Yung H. Wong Chapter 9: Screening for Chemical Inhibitors of Heterologous Proteins Expressed in Yeast Using a Simple Growth-restoration Assay Aruna D. Balgi and Michel Roberge Chapter 10: Assay for Isolation of Inhibitors of Her2-kinase Expression Gabriela Chiosis and Adam B. Keeton Chapter 11: ImageJ: A Primer Katherine Luby-Phelps Chapter 12: High-content Screening: Flow Cytometry Analysis Bruce S. Edwards, Susan M. Young, Irena Ivnitsky-Steele, Richard D. Ye, Eric R. Prossnitz, and Larry A. Sklar Chapter 13: High-throughput Real-time PCR for Detection of Gene-expression Levels Bridget K. Wagner and Zoltan Arany Chapter 14: Interpretation of Uniform-well Readouts Serene Josiah Chapter 15: Extracting Rich Information from Images Anne E. Carpenter
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