Phage display : a laboratory manual
Author(s)
Bibliographic Information
Phage display : a laboratory manual
Cold Spring Harbor Laboratory Press, c2001
- : pbk
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Note
Includes bibliographical references and index
"This book has been reproduced using print on demand technology"--Publisher's note
Description and Table of Contents
Description
Phage-display technology has begun to make critical contributions to the study of molecular recognition. DNA sequences are cloned into phage, which then present on their surface the proteins encoded by the DNA. Individual phage are rescued through interaction of the displayed protein with a ligand, and the specific phage is amplified by infection of bacteria. Phage-display technology is powerful but challenging and the aim of this manual is to provide comprehensive instruction in its theoretical and applied so that any scientist with even modest molecular biology experience can effectively employ it. The manual reflects nearly a decade of experience with students of greatly varying technical expertise andexperience who attended a course on the technology at Cold Spring Harbor Laboratory. Phage-display technology is growing in importance and power. This manual is an unrivalled source of expertise in its execution and application.
Table of Contents
SECTION 1 - PHAGE DISPLAY Chapter 1. Filamentous Phage Biology Chapter 2. Phage-display Vectors Chapter 3. Antibody Libraries Chapter 4. Peptide Libraries Chapter 5. Functional Domains and Scaffolds Chapter 6. Gene Fragment Libraries and Genomic and cDNA Expression Cloning SECTION 2 - ANTIBODY LIBRARIES Chapter 7. Overview: Amplification of Antibody Genes Chapter 8. Generation of Antibody Libraries: Immunization, RNA Preparation, and cDNA Synthesis Chapter 9. Generation of Antibody Libraries: PCR Amplification and Assembly of Light- And Heavy-chain Coding Sequences Chapter 10. Selection from Antibody Libraries Chapter 11. Analysis of Selected Antibodies Chapter 12. Production and Purification of Fab and scFv Chapter 13. Antibody Engineering SECTION 3 - PEPTIDE LIBRARIES Chapter 14. Overview: Peptide Libraries Chapter 15. General Phage Methods Chapter 16. Production of Peptide Libraries Chapter 17. Screening Peptide Libraries Chapter 18. Analysis of Phage-borne Peptides Chapter 19. Construction and Use of pIII-displayed Peptide Libraries SECTION 4 - OTHER LIBRARIES AND OTHER METHODS OF PANNING Chapter 20. Construction and Selection from Gene Fragment Phage-display Expression Libraries Chapter 21. Construction and Selection from cDNA Phage-display Expression Libraries Chapter 22. In Vivo Selection of Phage-display Libraries Chapter 23. Cell-surface Selection and Analysis of Monoclonal Antibodies from Phage Libraries SECTION 5 - APPENDICES Appendix 1. Useful Information Appendix 2. Recipes Appendix 3. General Procedures Appendix 4. Cautions Appendix 5. Suppliers Appendix 6. Trademarks
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