Human embryogenesis : methods and protocols
Author(s)
Bibliographic Information
Human embryogenesis : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, 550）（Springer protocols）
Humana , Springer [distributor], 2009
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Note
Includes bibliographical references and index
Description and Table of Contents
Description
Despite its inherent controversy, the exploration of the human embryo can unlock many of the answers to our deepest biological questions. In Human Embryogenesis: Methods and Protocols, internationally recognized researchers contribute detailed methods to analyze various aspects of the embryogenesis process. While comprehensively covering subjects such as the molecular mechanisms of embryonic development, in vitro fertilization, cloning, and the laws and ethical considerations of working with embryos, the volume also addresses critical features of fetal and placental development as well as of uterine biology. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and state-of-the-art, Human Embryogenesis: Methods and Protocols provides a firm foundation for the successful analysis of the embryogenesis process and an easily accessible description of the limitations and advantages of the techniques proposed, certain to aid all those who wish to further unravel the mysteries of human embryogenesis.
Table of Contents
Part I. Introduction: What is Human Embryogenesis?
Human Embryogenesis: Overview
Cathy Vaillancourt and Julie Lafond
Part II. Studying Embryo Implantation: from Trophoblasts to Embryo
Differential Gene Expression in the Uterus and Blastocyst During the Reactivation of Embryo Development in a Model of Delayed Implantation.
Pavine L.C. Lefevre and Bruce D. Murphy
Coculture of Decidua and Trophoblast to Study Proliferation and Invasion
Marie Cohen and Paul Bischof
Isolation and Culture of Term Human Cytotrophoblast Cells and In Vitro Methods for Studying Human Cytotrophoblast Cells Calcium Uptake
Frederique Le Bellego, Cathy Vaillancourt and Julie Lafond
Endocrinology and Cell Signaling in Human Villous Trophoblast
Catherine Mounier, Benoit Barbeau, Cathy Vaillancourt and Julie Lafond
Gestation-Induced Uterine Vascular Remodeling
Pierre-Andre Scott, Mylene Provencher, Pascale Guerin and Jean St-Louis
Part III. Steriodogenesis and Reproductive System
Placental and Fetal Steriodogenesis
J. Thomas Sanderson
Current Methods in Investigating the Development of the Female Reproductive System
Patrick J. Devine, Patricia B. Hoyer and Aileen F. Keating
A Revised and Improved Method for the Isolation of Seminiferous Tubule-Enriched Fractions that Preserves the Phosphorylated and Glycosylated Forms of Proteins
Casimir D. Akpov and R.-Marc Pelletier
A Novel Technical Approach for the Measurement of Individual ACAT-1 and ACAT-2 Enzymatic Activity in the Testis
Li Chen, Julie Lafond and R.-Marc Pelletier
Part IV. From Embryo to Fetal Development
GeneticDissection of Caenorhabditis elegans Embryogenesis Using RNA Interference and Flow-Cytometry
Elodie L. Boulier and Sarah Jenna
Immune System: Maturation of Myeloid Cells
Jamila Ennaciri and Denis Girard
Functional Development of Human Fetal Gastrointestinal Tract
Emile Levy, Edgard Delvin, Daniel Menard and Jean-Francois Beaulieu
Renal and Cardiac Na+-K+-ATPase and Aconitase in a Rat Model of Fetal Programming
Rebecca Gaudet and Michele Brochu
Part V. Embryotoxicity, In Vitro Fertilization, Cloning, and Ethics
Assessment of Embryotoxicity Using Mouse Embryo Culture
Louise M. Winn and Emily W.Y. Tung
Fertilization In Vitro
Yinzhong Bing and Rodney J. Ouellette
Animal Cloning by Somatic Cell Nuclear Transfer
Lawrence C. Smith and Jae-Gyu Yoo
The Human Embryo: Ethical and Legal Aspects
Bartha Maria Knoppers, Sylvie Bordet and Rosario Isasi
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