Essential zebrafish methods : genetics and genomics
著者
書誌事項
Essential zebrafish methods : genetics and genomics
（Reliable lab solutions）
Academic Press, 2009
大学図書館所蔵 全7件
内容説明・目次
内容説明
Due to its prolific reproduction and the external development of the transparent embryo, the zebrafish is the prime model for genetic and developmental studies, as well as research in genomics. While genetically distant from humans, nonetheless the vertebrate zebrafish has comparable organs and tissues that make it the model organism for study of vertebrate development.This book, one of two new volumes in the Reliable Lab Solutions series dealing with zebrafish, brings together a robust and up-to-date collection of time-tested methods presented by the world's leading scientists. Culled from previously published chapters in Methods in Cell Biology and updated by the original authors where relevant, it provides a comprehensive collection of protocols describing the most widely used techniques relevant to the study of zebrafish genetics and genomics. The methods in this volume were hand-selected by the editors, whose goal was to a provide a handy and cost-effective collection of fail-safe methods, tips, and "tricks of the trade" to both experienced researchers and more junior members in the lab.
目次
1. Haploid Screens and Gamma-Ray Mutagenesis
Charline Walker
2. Positional Cloning of Mutated Zebrafish Genes
Will Talbot
3. The Zebrafish Genome
John Postlethwait
4. Retroviral-Mediated Insertional Mutogenesis in Zebrafish
Adam Amsterdam
5. Genetic Screens for Maternal-Effect Mutations
Francisco Pelegri
6. Behavior Screening Assays in Zebrafish
Herwig Baier
7. A High-Throughput Method for Identifying N-Ethyl-N-Nitrosourea (ENU)-Induced Point Mutations in Zebrafish
Cecelia B. Moens
8. Transgenesis and Gene Trap Methods in Zebrafish by Using the Tol2 Transposable Element
Koichi Kawakami
9. Molecular Cytogenetic Methodologies and a Bacterial Artificial Chromosome (BAC) Probe Panel Resource for Genomic Analysis in Zebrafish
Charles Lee
10. The Zon Laboratory Guide to Positional Cloning in Zebrafish
Leonard I. Zon
11. Sleeping Beauty Transposon for Efficient Gene Delivery
Stephen C. Ecker
12. Transgene Manipulation in Zebrafish by Using Recombinases
Gary W. Stuart
13. Highly Efficient Zebrafish Transgenesis Mediated by the Meganuclease I-SceI
Joachim Wittbrodt
14. Cloning Zebrafish by Nuclear Transfer
Shuo Lin
15. Spatial and Temporal Expression of the Zebrafish Genome by Large-Scale In Situ Hybridization Screening
Christine Thisse
16. Genetic Backgrounds, Standard Lines, and Husbandry of Zebrafish
Bill Trevarrow
17. Common Diseases of Laboratory Zebrafish
Jennifer L. Matthews
18. Zebrafish Sperm Cryopreservation
Thomas Look
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