Essential zebrafish methods : genetics and genomics

Author(s)

• Detrich, H. William
• Westerfield, Monte
• Zon, Leonard I.

Bibliographic Information

Essential zebrafish methods : genetics and genomics

edited by H. Detrich, III, Monte Westerfield, Leonard I. Zon

（Reliable lab solutions）

Academic Press, 2009

Description and Table of Contents

Description

Due to its prolific reproduction and the external development of the transparent embryo, the zebrafish is the prime model for genetic and developmental studies, as well as research in genomics. While genetically distant from humans, nonetheless the vertebrate zebrafish has comparable organs and tissues that make it the model organism for study of vertebrate development.This book, one of two new volumes in the Reliable Lab Solutions series dealing with zebrafish, brings together a robust and up-to-date collection of time-tested methods presented by the world's leading scientists. Culled from previously published chapters in Methods in Cell Biology and updated by the original authors where relevant, it provides a comprehensive collection of protocols describing the most widely used techniques relevant to the study of zebrafish genetics and genomics. The methods in this volume were hand-selected by the editors, whose goal was to a provide a handy and cost-effective collection of fail-safe methods, tips, and "tricks of the trade" to both experienced researchers and more junior members in the lab.

Table of Contents

1. Haploid Screens and Gamma-Ray Mutagenesis Charline Walker 2. Positional Cloning of Mutated Zebrafish Genes Will Talbot 3. The Zebrafish Genome John Postlethwait 4. Retroviral-Mediated Insertional Mutogenesis in Zebrafish Adam Amsterdam 5. Genetic Screens for Maternal-Effect Mutations Francisco Pelegri 6. Behavior Screening Assays in Zebrafish Herwig Baier 7. A High-Throughput Method for Identifying N-Ethyl-N-Nitrosourea (ENU)-Induced Point Mutations in Zebrafish Cecelia B. Moens 8. Transgenesis and Gene Trap Methods in Zebrafish by Using the Tol2 Transposable Element Koichi Kawakami 9. Molecular Cytogenetic Methodologies and a Bacterial Artificial Chromosome (BAC) Probe Panel Resource for Genomic Analysis in Zebrafish Charles Lee 10. The Zon Laboratory Guide to Positional Cloning in Zebrafish Leonard I. Zon 11. Sleeping Beauty Transposon for Efficient Gene Delivery Stephen C. Ecker 12. Transgene Manipulation in Zebrafish by Using Recombinases Gary W. Stuart 13. Highly Efficient Zebrafish Transgenesis Mediated by the Meganuclease I-SceI Joachim Wittbrodt 14. Cloning Zebrafish by Nuclear Transfer Shuo Lin 15. Spatial and Temporal Expression of the Zebrafish Genome by Large-Scale In Situ Hybridization Screening Christine Thisse 16. Genetic Backgrounds, Standard Lines, and Husbandry of Zebrafish Bill Trevarrow 17. Common Diseases of Laboratory Zebrafish Jennifer L. Matthews 18. Zebrafish Sperm Cryopreservation Thomas Look

by "Nielsen BookData"

Details

• NCID: BA91785420
• ISBN:
  • 9780123751607
• Country Code: ne
• Title Language Code: eng
• Text Language Code: eng
• Place of Publication: Amsterdam
• Pages/Volumes: xviii, 373 p., [6] p. of plates
• Size: 24 cm
• Classification:
  • DC22 : 572.817482
• Subject Headings:
  • LCSH : Zebra danio -- Genetics -- Research
  • LCSH : Zebra danios as laboratory animals -- Laboratory manuals
• Parent Bibliography ID:
  • BA91412853