Methods and protocols
著者
書誌事項
Methods and protocols
（Methods in molecular biology / John M. Walker, series editor, 579-580 . Lipidomics）（Springer protocols）
Humana Press, c2009-
- v. 1
- v. 2
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注記
Includes bibliographical references and index
内容説明・目次
- 巻冊次
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v. 1 ISBN 9781607613213
内容説明
Essential in biological functions like cell signaling and, when disturbed, a likely cause of disease, lipids have proven to be a vital force in cell biology. In Lipidomics: Methods and Protocols, an international panel of experts present a wide variety of reviewed as well as unpublished data on isolation techniques, structural analysis, lipid rafts, lipid trafficking and profiling, biomarkers, lipid peroxidation, biostatistics applied to lipids, software tools, and bioinformatics. These studies range from simple systems, such as in yeast, to complex biological models. Samples used in these studies include: mitochondria, microsomes, endosomal membranes, RBS and plasma membranes, vesicles, caveolae, liposomes, macrophages, meibum, brain, microglia, retina, adipose tissue, keratinocytes, pulmonary surfactant, and hepatic cultures. Volume 1 focuses on shotgun and global lipidomics, analytical approaches, and lipid maps. Written in the highly successful Methods in Molecular Biology (TM) series format, the chapters include useful introductions to their respective topics, lists of the necessary equipment and materials, step-by-step, readily reproducible laboratory protocols, and authoritative notes on troubleshooting and avoiding common pitfalls.
Comprehensive and cutting-edge, Lipidomics: Methods and Protocols will support researchers who wish to increase their utilization of lipidomics, which will certainly lead to more powerful technology, improved diagnostic-prognostic capabilities, and the identification of new valuable products.
目次
Part I: Shotgun and Global Lipidomics 1. Examination of the Brain Mitochondrial Lipidome Using Shotgun Lipidomics Michael A. Kiebish, Xianlin Han, and Thomas N. Seyfried 2. Tracking the Glycerophospholipid Distribution of Docosahexaenoic Acid by Shotgun Lipidomics Todd W. Mitchell 3. Global Analysis of Retina Lipids by Complementary Precursor Ion and Neutral Loss Mode Tandem Mass Spectrometry Julia V. Busik, Gavin E. Reid, and Todd A. Lydic 4. Combining Lipidomics and Proteomics of Human Cerebrospinal Fluids Alfred N. Fonteh and Rachel D. Fisher Part II: Analytical Approaches 5. Lipid Profiling Using 2-Dimensional Heteronuclear Single Quantum Coherence NMR Engy A. Mahrous, Robin B. Lee, and Richard E. Lee 6. Capabilities and Drawbacks of Phospholipid Analysis by MALDI-TOF Mass Spectrometry Beate Fuchs, Ariane Nimptsch, Rosmarie Suss, and Jurgen Schiller 7. Lipidomics of the Red Cell in Diagnosis of Human Disorders Peter J. Quinn, Dominique Rainteau, and Claude Wolf 8. HPLC/MS/MS-Based Approaches for Detection and Quantification of Eicosanoids Susanna L. Lundstroem, Fabio L. D'Alexandri, Kasem Nithipatikom, Jesper Z. Haeggstroem, Asa M. Wheelock, and Craig E. Wheelock 9. Brain Phosphoinositide Extraction, Fractionation, and Analysis by MALDI-TOF MS Roy A. Johanson and Gerard T. Berry 10. Lipidomic Analysis of Biological Samples by Liquid Chromatography Coupled to Mass Spectrometry Giuseppe Astarita, Faizy Ahmed, and Daniele Piomelli 11. Lipidomic Analysis of Human Meibum Using HPLC-MSn Igor A. Butovich 12. Lipid Geographical Analysis of the Primate Macula by Imaging Mass Spectrometry Timothy J. Garrett and William W.Dawson 13. A Novel Role for Nutrition in the Alteration of Functional Microdomains on the Cell Surface Wooki Kim, Robert S. Chapkin, Rola Barhoumi, and David W.L. Ma 14. Lipidomic Analysis of Prostanoids by Liquid Chromatography-Electrospray Tandem Mass Spectrometry Anna Nicolaou, Mojgan Masoodi, and Adnan Mir 15. Qualitative and Quantitative Analyses of Phospholipids by LC-MS for Lipidomics Hiroki Nakanishi, Hideo Ogiso, and Ryo Taguchi 16. Determination of Fatty Acid Profiles and TAGs in Vegetable Oils by MALDI-TOF/MS Fingerprinting Zeev Wiesman and Bishnu P. Chapagain 17. Dynamics of Adipose Tissue Development by 2H2O Labeling Etienne Pouteau, Carine Beysen, Nabil Saad, and Scott Turner 18. Analysis of Lipid Particles from Yeast Melanie Connerth, Karlheinz Grillitsch, Harald Koefeler, and Gunther Daum 19. Mammalian Fatty Acid Elongases Donald B. Jump 20. Membrane Lipidomics and the Geometry of Unsaturated Fatty Acids : From Biomimetic Models to Biological Consequences Carla Ferreri and Chryssostomos Chatgilialoglu 21. On-Line Ozonolysis Methods for the Determination of Double Bond Position in Unsaturated Lipids Michael C. Thomas, Todd W. Mitchell, and Stephen J. Blanksby 22. Comprehensive Quantitative Analysis of Bioactive Sphingolipids High-Performance Liquid Chromatography-Tandem Mass Spectrometry Jacek Bielawski, Jason S. Pierce, Justin Snider, Barbara Rembiesa, Zdzislaw M. Szulc, and Alicja Bielawska 23. The Use of Charged Aerosol Detection with HPLC for the Measurement of Lipids Marc Plante, Bruce Bailey, and Ian Acworth Part III: Lipid Maps 24. Non-Invasive Mapping of Lipids in Plant Tissue Using Magnetic Resonance
- 巻冊次
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v. 2 ISBN 9781617796555
内容説明
Essential in biological functions like cell signaling and, when disturbed, a likely cause of disease, lipids have proven to be a vital force in cell biology. In Lipidomics: Methods and Protocols, an international panel of experts present a wide variety of reviewed as well as unpublished data on isolation techniques, structural analysis, lipid rafts, lipid trafficking and profiling, biomarkers, lipid peroxidation, biostatistics applied to lipids, software tools, and bioinformatics. These studies range from simple systems, such as in yeast, to complex biological models. Samples used in these studies include: mitochondria, microsomes, endosomal membranes, RBS and plasma membranes, vesicles, caveolae, liposomes, macrophages, meibum, brain, microglia, retina, adipose tissue, keratinocytes, pulmonary surfactant, and hepatic cultures. Volume 2 focuses on oxidized lipids, trafficking and profiling, software, bioinformatics, and biostatistics. Written in the highly successful Methods in Molecular Biology (TM) series format, the chapters include useful introductions to their respective topics, lists of the necessary equipment and materials, step-by-step, readily reproducible laboratory protocols, and authoritative notes on troubleshooting and avoiding common pitfalls.
Comprehensive and cutting-edge, Lipidomics: Methods and Protocols will support researchers who wish to increase their utilization of lipidomics, which will certainly lead to more powerful technology, improved diagnostic-prognostic capabilities, and the identification of new valuable products.
目次
Part I: Oxidized Lipids 1. F2-Isoprostanes: Sensitive Biomarkers of Oxidative Stress in Vitro and in Vivo: A Gas Chromatography-Mass Spectrometric Approach Ingrid Wiswedel 2. Volatile Oxylipins and Related Compounds Formed Under Stress in Plants Kenji Matsui, Koichi Sugimoto, Pattana Kakumyan, Sergey A. Khorobrykh, and Jun'ichi Mano 3. Quantification of Lysophosphatidylcholine Species by High Throughput Electrospray Ionization Tandem Mass Spectrometry (ESI-MS/MS) Gerhard Liebisch and Gerd Schmitz 4. Use of Lipidomics for Analyzing Glycerolipid and Cholesteryl Ester Oxidation by Gas Chromatography, HPLC, and On-line MS Arnis Kuksis, Jukka-Pekka Suomela, Marko Tarvainen, and Heikki Kallio 5. Lipid Raft Redox Signaling Platforms in Plasma Membrane Fan Yi, Si Jin, and Pin-Lan Li 6. Mass Spectrometry Analysis of Polyisoprenoids Alcohols and Carotenoids via ESI(Li+)-MS/MS Fabio Luiz D'Alexandri, Renata Tonhosolo, Emilia A. Kimura, and Alejandro Miguel Katzin 7. Detection of a Lipid-Lysine Adduct Family with an Amide-Bond as the Linkage: Novel Markers for Lipid-Derived Protein Modifications Yoji Kato and Toshihiko Osawa 8. Assessing the Neuroprotective Effect of Antioxidative Food Factors by Application of Lipid-Derived Dopamine Modification Adducts Xuebo Liu, Naruomi Yamada, and Toshihiko Osawa 9. Mass-Spectrometric Characterization of Phospholipids and Their Hydroperoxide-Derivatives in Vivo: Effects of Total Body Irradiation Yulia Y. Tyurina, Vladimir A. Tyurin, Valentina I. Kapralova, Andrew A. Amoscato, Michael W. Epperly, Joel S. Greenberger, and Valerian E. Kagan Part II: Trafficking and Profiling 10. Comprehensive mRNA Profiling of Lipid-RelatedGenes in Microglia and Macrophages Using Taqman Arrays Richard Mauerer, Yana Walczak, and Thomas Langmann 11. Imaging Lipid Membrane Domains with Lipid-Specific Probes Francoise Hullin-Matsuda, Reiko Ishitsuka, Miwa Takahashi and Toshihide Kobayashi 12. Monitoring Sterol Uptake, Acetylation, and Export in Yeast Vineet Choudhary and Roger Schneiter 13. Methods to Monitor Fatty Acid Transport Proceeding Through Vectorial Acylation Elsa Arias-Barrau, Concetta C. DiRusso, and Paul N. Black 14. Activity-Based Profiling of Lipases in Living Cells Maximilian Schicher, Iris Jesse, and Ruth Birner-Gruenberger 15. Histochemistry and Lipid Profiling Combine for Insights into Aging and Age-related Maculopathy Christine A. Curcio, Martin Rudolf, and Lan Wang Part III: Software and Bioinformatics 16. Instrument-Independent Software Tools for the Analysis of MS/MS and LC-MS Lipidomics Data Perttu Haimi, Krishna Chaithanya B., Ville Kainu, Martin Hermansson, and Pentti Somerharju 17. Computer-Assisted Interpretation of Triacylglycerols Mass Spectra Josef Cvacka and Edita Kofronova 18. Visualization of Complex Processes in Lipid Systems Using Computer Simulations and Molecular Graphics Jelena Telenius, Ilpo Vattulainen, and Luca Monticelli 19. Bioinformatics Strategies for the Analysis of Lipids Craig E. Wheelock, Susumu Goto, Laxman Yetukuri, Fabio Luiz D'Alexandri, Christian Klukas, Falk Schreiber, and Matej Oresic Part IV: Biostatistics 20. The Effect of Lipid Adjustment on the Analysis of Environmental Contaminants and the Outcome of Human Health Risks Audrey J. Gaskins and Enrique F. Schisterman
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