Functional glycomics : methods and protocols
著者
書誌事項
Functional glycomics : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, 600）（Springer protocols）
Humana, c2010
- : [hbk]
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
The study of functional glycomics requires the continuous development of rapid and sensitive methods for the identification of glycan structures and integration to structure-function relationships. In Functional Glycomics: Methods and Protocols, a panel of world-renowned experts provide new developments and emerging glycomics techniques in the form of detailed protocols exploring the fundamental challenges and most cutting-edge novel applications. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters in this volume present brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes sections, highlighting key tips on troubleshooting and avoiding known pitfalls.
Authoritative and easy to use, Functional Glycomics: Methods and Protocols serves as an ideal reference for scientists working in biochemistry, molecular biology, cell biology, immunology, microbiology, and virology and a guide to appropriate techniques and the design of achievable research plans in this vital field.
目次
1. Functional Glycomics and Glycobiology: An Overview
Jianjun Li and James C. Richards
2. Historical Overview of Glycoanalysis
Alicia M. Bielik and Joseph Zaia
3. Quantitative Glycomics
Ron Orlando
4. Human Gangliosides and Bacterial Lipo-oligosaccharides in the Development of Autoimmune Neuropathies
Nobuhiro Yuki
5. Biotinylated Multivalent Glycoconjugates for Surface Coating
Alexander Chinarev, Oxana Galanina, and Nicolai Bovin
6. Profiling LPS Glycoforms of Non-typeable Haemophilus influenzae by Multiple-Stage Tandem Mass Spectrometry
Elke K.H. Schweda and James C. Richards
7. In vitro Reconstitution of Escherichia coli O86 O Antigen Repeating Unit
Weiqing Han, Lei Li, Nicholas Pettit, Wen Yi, Robert Woodward, Xianwei Liu, Wanyi Guan, Veer Bhatt, Jing Katherine Song, and Peng George Wang
8. Glycoprotein Characterization
Susan M. Twine, Luc Tessier, and John F. Kelly
9. N-Linked Glycoprotein Analysis Using Dual-Extraction Ultrahigh-Performance Liquid Chromatography and Electrospray Tandem Mass Spectrometry
S. O. Siu, Maggie P. Y. Lam, Edward Lau, William S.B. Yeung, David M. Cox, and Ivan K. Chu
10. Microarray-Based Study of Carbohydrate-Protein Binding
Zhenxin Wang and Jingqing Gao
11. The Application of NMR Spectroscopy to Functional Glycomics
Jean-Robert Brisson, Evgeny Vinogradov, David McNally, Nam Huan Khieu, Ian Schoenhofen, Susan Logan, and Harold Jarrell
12. Metabolomics in Glycomics
Evelyn C. Soo and Joseph P. M. Hui
13. Characterization of Lipid-Linked Oligosaccharides by Mass Spectrometry
Christopher W. Reid, Jacek Stupak, and Christine M. Szymanski
14. A Glycomics Approach to the Discovery of Potential Cancer Biomarkers
Hyun Joo An and Carlito B. Lebrilla
15. Extraction of Chondroitin/Dermatan Sulfate Glycosaminoglycans from Connective Tissue for Mass Spectrometric Analysis
Alicia M. Bielik and Joseph Zaia
16. N-Linked Protein Glycosylation in a Bacterial System
Harald Nothaft, Xin Liu, David J. McNally, and Christine M. Szymanski
17. Characterization of Polysaccharides Using Mass Spectrometry for Bacterial Serotyping
Eleonora Altman and Jianjun Li
18. Is Permethylation Strategy Always Applicable to Protein N-Glycosylation Study?: A Case Study on the O-Acetylation of Sialic Acid in Fish Serum Glycans
Xin Liu and Luis Afonso
19. Bioinformatics in Glycomics: Glycan Characterization with Mass Spectrometric Data Using SimGlycan (TM)
Arun Apte and Ningombam Sanjib Meitei
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