Peptidomics : methods and protocols
Author(s)
Bibliographic Information
Peptidomics : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, 615）（Springer protocols）
Humana, c2010
Available at 2 libraries
Note
Includes bibliographical references and index
Description and Table of Contents
Description
Despite being known and studied for years, peptides have never before attracted enough attention to necessitate the invention of the term "peptidomics" in order to specify the study of the complement of peptides from a cell, organelle, tissue or organism. In Peptidomics: Methods and Protocols, expert researchers present a comprehensive range of analytical techniques for the analysis of the peptide contents of complex biological samples with an emphasis often on higher throughput techniques, suitable for the analysis of large numbers of peptides typically present in the peptidomes. Encompassing a number of species ranging from bacteria to man, the methods presented intensively cover topics such as organism handling, tissue and organ dissection, cellular and subcellular fractionation, peptide extraction, fractionation and purification, structural characterization, molecular cloning, and sequence analysis. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Comprehensive and cutting-edge, Peptidomics: Methods and Protocols brings this ten year old field fully up-to-date in order to inspire novices and experts alike with the easy-to-follow practical advice needed to set up and carry out analysis of the peptide contents of complex biological samples.
Table of Contents
Part I: Introduction
1. Peptidomics: Divide et Impera
Mikhail Soloviev
Part II: From Bacteria to Men
2. Performing Comparative Peptidomics Analyses of Salmonella from Different Growth Conditions
Joshua N. Adkins, Heather Mottaz, Thomas O. Metz, Charles Ansong, Nathan P. Manes, Richard D. Smith, and Fred Heffron
3. Approaches to Identify Endogenous Peptides in the Soil Nematode Caenorhabditis elegans
Steven J. Husson, Elke Clynen, Kurt Boonen, Tom Janssen, Marleen Lindemans, Geert Baggerman, and Liliane Schoofs
4. Mass Spectrometric Analysis of Molluscan Neuropeptides
Ka Wan Li and August B. Smit
5. Monitoring Neuropeptides in vivo via Microdialysis and Mass Spectrometry
Heidi L. Behrens and Lingjun Li
6. Protocols for Peptidomic Analysis of Spider Venoms
Liang Songping
7. Purification and Characterization of Biologically Active Peptides from Spider Venoms
Alexander A. Vassilevski, Sergey A. Kozlov, Tsezi A. Egorov, and Eugene V. Grishin
8. MALDI-TOF Mass Spectrometry Approaches to the Characterization of Insect Neuropeptides
Robert J. Weaver and Neil Audsley
9. Direct MALDI-TOF Mass Spectrometric Peptide Profiling of Neuroendocrine Tissue of Drosophila
Christian Wegener, Susanne Neupert, and Reinhard Predel
10. Direct Peptide Profiling of Brain Tissue by MALDI-TOF Mass Spectrometry
Joachim Schachtner, Christian Wegener, Susanne Neupert, and Reinhard Predel
11. Peptidomic Analysis of Single Identified Neurons
Susanne Neupert and Reinhard Predel
12. Identification and Analysis of Bioactive Peptides in Amphibian Skin Secretions
J. Michael Conlon and Jerome Leprince
13. An Efficient Protocol for DNA Amplification of Multiple Amphibian Skin Antimicrobial PeptidecDNAs
Shawichi Iwamuro and Tetsuya Kobayashi
14. Combined Peptidomics and Genomics Approach to the Isolation of Amphibian Antimicrobial Peptides
Ren Lai
15. Identification and Relative Quantification of Neuropeptides from the Endocrine Tissues
Kurt Boonen, Steven J. Husson, Bart Landuyt, Geert Baggerman, Eisuke Hayakawa, Walter H.M.L. Luyten, and Liliane Schoofs
16. Peptidome Analysis of Mouse Liver Tissue by Size Exclusion Chromatography Prefractionation
Lianghai Hu, Mingliang Ye, and Hanfa Zou
17. Rat Brain Neuropeptidomics: Tissue Collection, Protease Inhibition, Neuropeptide Extraction, and Mass Spectrometric Analysis
Robert M. Sturm, James A. Dowell, and Lingjun Li
18. Quantitative Neuroproteomics of the Synapse
Dinah Lee Ramos-Ortolaza, Ittai Bushlin, Noura Abul-Husn, Suresh P. Annagudi, Jonathan Sweedler, and Lakshmi A. Devi
19. Peptidomics Analysis of Lymphoblastoid Cell Lines
Anne Fogli and Philippe Bulet
20. Peptidomics: Identification of Pathogenic and Marker Peptides
Yang Xiang, Manae S. Kurokawa, Mie Kanke, Yukiko Takakuwa, and Tomohiro Kato
Part III: Tools and Approaches
21. Peptidomic Approaches to the Identification and Characterization of Functional Peptides in Hydra
Toshio Takahashi and Toshitaka Fujisawa
22. Immunochemical Methods for the Peptidomic Analysis of Tachykinin Peptides and their Precursors
Nigel M. Page and Nicola J. Weston-Bell
23. Affinity Peptidomics: Peptide Selection and Affinity Capture on Hydrogels and Microarrays
Fan Zhang, Anna Dulneva, Julian Bailes, and Mikhail Soloviev
24. In situ Biosynthesis of Peptide Arrays
Mingyue He and Oda Stoevesandt
25. Bioinformatic Approaches to the Identification of Novel Neuropeptide Precursors
Elke Clynen, Feng
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