Human embryonic stem cell protocols
著者
書誌事項
Human embryonic stem cell protocols
(Methods in molecular biology / John M. Walker, series editor, 584)(Springer protocols)
Humana Press, c2010
2nd ed
大学図書館所蔵 全4件
  青森
  岩手
  宮城
  秋田
  山形
  福島
  茨城
  栃木
  群馬
  埼玉
  千葉
  東京
  神奈川
  新潟
  富山
  石川
  福井
  山梨
  長野
  岐阜
  静岡
  愛知
  三重
  滋賀
  京都
  大阪
  兵庫
  奈良
  和歌山
  鳥取
  島根
  岡山
  広島
  山口
  徳島
  香川
  愛媛
  高知
  福岡
  佐賀
  長崎
  熊本
  大分
  宮崎
  鹿児島
  沖縄
  韓国
  中国
  タイ
  イギリス
  ドイツ
  スイス
  フランス
  ベルギー
  オランダ
  スウェーデン
  ノルウェー
  アメリカ
注記
Previous ed.: 2006
Includes bibliographical references and index
内容説明・目次
内容説明
Despite political and ethical controversies surrounding the study of human embryonic stem (hES) cells, new freedoms in regard to using them for research has allowed interest to remain high in understanding the regulatory mechanisms of stem cell self-renewal, their differentiation along various lineages, and their potential use in regenerative medicine. In Human Embryonic Stem Cell Protocols, Second Edition, internationally respected researchers expand upon the popular first edition and describe in detail their most useful techniques for the molecular and cellular manipulation of these intriguing cells. This diverse collection of readily reproducible methods has been optimized for the derivation, characterization, and differentiation of hES cells, with special attention given to regenerative medicine applications. As a volume of the Methods in Molecular Biology™ series, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Comprehensive and cutting-edge, Human Embryonic Stem Cell Protocols, Second Edition offers both novice and expert researchers powerful tools essential to understanding the maintenance and differentiation of human embryonic stem cells, as well as their applications in regenerative medicine today.
目次
Human Embryo Culture and Assessment for the Derivation of Embryonic Stem Cells (ESC).- Derivation of Human Embryonic Stem Cell Lines from Vitrified Human Embryos.- Growth of Human Embryonic Stem Cells Using Derivates of Human Fibroblasts.- In Vitro Neural Differentiation of Human Embryonic Stem Cells Using a Low-Density Mouse Embryonic Fibroblast Feeder Protocol.- Optimization of Physiological Xenofree Molecularly Defined Media and Matrices to Maintain Human Embryonic Stem Cell Pluripotency.- Serum-Free and Feeder-Free Culture Expansion of Human Embryonic Stem Cells.- Single Cell Enzymatic Dissociation of Human Embryonic Stem Cells: A Straightforward, Robust, and Standardized Culture Method.- Monitoring Stemness in Long-Term hESC Cultures by Real-Time PCR.- Culture and Preparation of Human Embryonic Stem Cells for Proteomics-Based Applications.- A Two- and Three-Dimensional Approach for Visualizing Human Embryonic Stem Cell Differentiation.- Immunoflourescence and mRNA Analysis of Human Embryonic Stem Cells (hESCs) Grown Under Feeder-Free Conditions.- Study of Gap Junctions in Human Embryonic Stem Cells.- hESC Engineering by Integrase-Mediated Chromosomal Targeting.- Human Embryonic Stem Cell Differentiation on Periodontal Ligament Fibroblasts.- Generation of Neural Crest Cells and Peripheral Sensory Neurons from Human Embryonic Stem Cells.- Embryonic Stem Cells as a Model for Studying Melanocyte Development.- In Vitro Derivation of Chondrogenic Cells from Human Embryonic Stem Cells.- Vascular Differentiation of Human Embryonic Stem Cells in Bioactive Hydrogel-Based Scaffolds.- Differentiation of Neural Precursors and Dopaminergic Neurons from Human Embryonic Stem Cells.- Transplantation of Human Embryonic Stem Cells and Derivatives to the Chick Embryo.- Genetic Manipulation of Human Embryonic Stem Cells.- Genetic Manipulation of Human Embryonic Stem Cells in Serum and Feeder-Free Media.- Human-Induced Pluripotent Stem Cells: Derivation, Propagation, and Freezing in Serum- and Feeder Layer-Free Culture Conditions.
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