Human embryonic stem cell protocols
Author(s)
Bibliographic Information
Human embryonic stem cell protocols
(Methods in molecular biology / John M. Walker, series editor, 584)(Springer protocols)
Humana Press, c2010
2nd ed
Available at 4 libraries
  Aomori
  Iwate
  Miyagi
  Akita
  Yamagata
  Fukushima
  Ibaraki
  Tochigi
  Gunma
  Saitama
  Chiba
  Tokyo
  Kanagawa
  Niigata
  Toyama
  Ishikawa
  Fukui
  Yamanashi
  Nagano
  Gifu
  Shizuoka
  Aichi
  Mie
  Shiga
  Kyoto
  Osaka
  Hyogo
  Nara
  Wakayama
  Tottori
  Shimane
  Okayama
  Hiroshima
  Yamaguchi
  Tokushima
  Kagawa
  Ehime
  Kochi
  Fukuoka
  Saga
  Nagasaki
  Kumamoto
  Oita
  Miyazaki
  Kagoshima
  Okinawa
  Korea
  China
  Thailand
  United Kingdom
  Germany
  Switzerland
  France
  Belgium
  Netherlands
  Sweden
  Norway
  United States of America
Note
Previous ed.: 2006
Includes bibliographical references and index
Description and Table of Contents
Description
Despite political and ethical controversies surrounding the study of human embryonic stem (hES) cells, new freedoms in regard to using them for research has allowed interest to remain high in understanding the regulatory mechanisms of stem cell self-renewal, their differentiation along various lineages, and their potential use in regenerative medicine. In Human Embryonic Stem Cell Protocols, Second Edition, internationally respected researchers expand upon the popular first edition and describe in detail their most useful techniques for the molecular and cellular manipulation of these intriguing cells. This diverse collection of readily reproducible methods has been optimized for the derivation, characterization, and differentiation of hES cells, with special attention given to regenerative medicine applications. As a volume of the Methods in Molecular Biology™ series, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Comprehensive and cutting-edge, Human Embryonic Stem Cell Protocols, Second Edition offers both novice and expert researchers powerful tools essential to understanding the maintenance and differentiation of human embryonic stem cells, as well as their applications in regenerative medicine today.
Table of Contents
Human Embryo Culture and Assessment for the Derivation of Embryonic Stem Cells (ESC).- Derivation of Human Embryonic Stem Cell Lines from Vitrified Human Embryos.- Growth of Human Embryonic Stem Cells Using Derivates of Human Fibroblasts.- In Vitro Neural Differentiation of Human Embryonic Stem Cells Using a Low-Density Mouse Embryonic Fibroblast Feeder Protocol.- Optimization of Physiological Xenofree Molecularly Defined Media and Matrices to Maintain Human Embryonic Stem Cell Pluripotency.- Serum-Free and Feeder-Free Culture Expansion of Human Embryonic Stem Cells.- Single Cell Enzymatic Dissociation of Human Embryonic Stem Cells: A Straightforward, Robust, and Standardized Culture Method.- Monitoring Stemness in Long-Term hESC Cultures by Real-Time PCR.- Culture and Preparation of Human Embryonic Stem Cells for Proteomics-Based Applications.- A Two- and Three-Dimensional Approach for Visualizing Human Embryonic Stem Cell Differentiation.- Immunoflourescence and mRNA Analysis of Human Embryonic Stem Cells (hESCs) Grown Under Feeder-Free Conditions.- Study of Gap Junctions in Human Embryonic Stem Cells.- hESC Engineering by Integrase-Mediated Chromosomal Targeting.- Human Embryonic Stem Cell Differentiation on Periodontal Ligament Fibroblasts.- Generation of Neural Crest Cells and Peripheral Sensory Neurons from Human Embryonic Stem Cells.- Embryonic Stem Cells as a Model for Studying Melanocyte Development.- In Vitro Derivation of Chondrogenic Cells from Human Embryonic Stem Cells.- Vascular Differentiation of Human Embryonic Stem Cells in Bioactive Hydrogel-Based Scaffolds.- Differentiation of Neural Precursors and Dopaminergic Neurons from Human Embryonic Stem Cells.- Transplantation of Human Embryonic Stem Cells and Derivatives to the Chick Embryo.- Genetic Manipulation of Human Embryonic Stem Cells.- Genetic Manipulation of Human Embryonic Stem Cells in Serum and Feeder-Free Media.- Human-Induced Pluripotent Stem Cells: Derivation, Propagation, and Freezing in Serum- and Feeder Layer-Free Culture Conditions.
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