Microtubules, in vitro

著者

• Wilson, Leslie
• Correia, John J. (John Joseph)

書誌事項

Microtubules, in vitro

edited by Leslie Wilson, John J. Correia

（Methods in cell biology / edited by David M. Prescott, v. 95）

Elsevier/Academic Press, 2010

内容説明・目次

内容説明

There continues to be intense interest in the microtubule cytoskeleton; the assembly, structure and regulation of microtubules; and the numerous motors and accessory proteins that control cell cycle, dynamics, organization and transport. The field continues to grow and explore new aspects of these issues driven immensely by developments in optical imaging and tracking techniques. This volume (complemented by the forthcoming companion volume by Cassimeris and Tran) brings together current research and protocols in the field of microtoubules in vitro and will serve as a valuable tool for cell biologists, biophysicists and pharmacologists who study the microtubule cytoskeleton, as well as for researchers in the biomedical and biotechnology communities with interest in developing drugs that target microtubules, MAPS and motors.

目次

Section1: Isolation, Biochemistry and Characterization of Antibodies and Isotypes 1. Preparation of Microtubule Protein and Purified Tubulin from Bovine Brain by Cycles of Assembly and Disassembly and Phosphocellulose Chromatography Herbert P. Miller and Leslie Wilson 2. Measurement Of Ligand Binding To Tubulin By Sulfhydryl Reactivity Adrian Begaye and Dan L Sackett 3. Isolating Tubulin From Nonneural Sources Dan L. Sackett, Karl A. Werbovetz and Naomi S. Morrissette 4. Characterization of Anti-b -Tubulin Antibodies Anthony J. Spano* and Anthony Frankfurter 5. Expression profiling of tubulin isotypes and microtubule interacting proteins using real-time polymerase chain reaction Sharon Lobert, Laree Hiser and John J. Correia 6. Nondenaturing Electrophoresis As A Tool To Investigate Tubulin Complexes Monica Lopez-Fanarraga, Gerardo Carranza, Raquel Castano, Sofia Nolasco, J. Avila and J.C. Zabala 7 .Mass spectrometry analysis of c-terminal posttranslational Modifications of tubulins Redeker, Virginie 8. Methods in Tubulin Proteomics Leah M. Miller, Hui Xiao, Berta Burd, Susan Band Horwitz, Ruth Hogue Angeletti, Pascal Verdier-Pinard Section 2: Microtubule Structure and Dynamics 9. Cryo-EM Studies of Microtubule Structural Intermediates and Kinetochore-Microtubule Interactions Eva Nogales, Vincent H. Ramey and Hong-Wei Wang 10. High resolution imaging of microtubules and cytoskeleton structures by Atomic Force Microscopy Loic Hamon, Patrick A. Curmi, David Pastre 11. Using computational modeling to understand microtubule dynamics: a primer for cell biologists Holly V. Goodson and Ivan V. Gregoretti 12. Analysis of Dynamic Instability of Steady State Microtubules In Vitro by Video-Enhanced Differential Interference Contrast Microscopy with an Appendix by Emin Oroudjev Mythili Yenjerla, Manu Lopus and Leslie Wilson 13. Nanometer-resolution microtubule polymerization assays using optical tweezers and microfabricated barriers Blake D. Charlebois, Henry T. Schek III, Alan J. Hunt 14. Microtubule Dynamics Reconstituted in-vitro and Imaged y Single-Molecule Fluorescence Microscopy Christopher Gell, Volker Bormuth, Gary J. Brouhard, Daniel N. Cohen, Stefan Diez, Claire T. Friel, Jonne Helenius, Bert Nitzsche, Heike Petzold, Jan Ribbe, Erik Schaffer, Jeffrey H. Stear, Anastasiya Trushko, Vladimir Varga, Per O. Widlund, Marija Zanic, and Jonathon Howard 15. Studying kinesin motors by optical 3D-nanometry in gliding motility assays Bert Nitzsche, Volker Bormuth, Corina Brauer, Jonathon Howard, Leonid Ionov, Jacob Kerssemakers, Till Korten, Cecile Leduc, Felix Ruhnow, Stefan Diez Section 3: Drugs 16. Analysis of tubulin oligomers by Analytical Ultracentrifugation. J.J. Correia 17. Determination of Drug Binding to Microtubules in vitro Jennifer A. Smith and Mary Ann Jordan 18. Fluorescence Spectroscopic Methods to Analyze Drug-Tubulin Interactions Bhabatarak Bhattacharyya, Sonia Kapoor and Dulal Panda 19. A Tubulin polymerization micro-assay used to compare ligand efficacy Ashley Davis, Sarah Martinez, Daniel Nelson and Kim Middleton. 20. Fluorescent Taxoid Probes for Microtubule Research Isabel Barasoain, J. Fernando Diaz, and Jose M. Andreu 21. The binding of vinca domain agents to tubulin: structural and biochemical studies Anthony Cormie, Marcel Knossow, Chunguang Wang and Benoit Gigant Section 4: Interactions with Motors and MAPs 22. Probing Interactions of Tubulin with Small Molecules, Peptides and Protein Fragments by Solution Nuclear Magnetic Resonance Marie-Jeanne Clement, Philippe Savarin, Elisabeth Adjadj, Andre Sobel, Flavio Toma, Patrick A. Curmi 23. Microtubule and MAPs: thermodynamics of complex formation by AUC, ITC, fluorescence and NMR Francois Devred, Pascale Barbier, Daniel Lafitte, Isabelle Landrieu, Guy Lippens and Vincent Peyrot 24. Quantitative Analysis of MAP Mediated Regulation of Microtubule Dynamic Instability In Vitro - Focus on Tau Erkan Kiris, Donovan Ventimiglia and Stuart C. Feinstein 25. Structure and dynamics of the kinesin-microtubule interaction revealed by fluorescence polarization microscopy (FPM) Hernando Sosa, Ana B. Asenjo and Erwin J.G. Peterman 26. Multiple color single molecule TIRF imaging and tracking of MAPs and motors Jennifer L. Ross and Ram Dixit 27. Fluorescence microscopy assays on chemically functionalized surfaces for quantitative imaging of microtubule, motor and +TIP dynamics Peter Bieling, Ivo A. Telley, Christian Hentrich, Jacob Piehler and Thomas Surrey Section 5: Functional Extracts and Force Measurements 28. Quantitative Characterization of filament dynamics by single-molecule lifetime measurements. Leonid A. Mirny and Daniel J. Needleman 29. Extracting the mechanical properties of microtubules from thermal fluctuation measurements on an attached tracer particle. = Katja M. Taute, Francesco Pampaloni, Ernst-Ludwig Florin 30. In vitro assays to study force generation at dynamic microtubule ends Liedewij Laan and Marileen Dogterom 31. Reconstitution and functional analysis of kinetochore subcomplexes Daniel R. Gestaut, Jeremy Cooper, Charles L. Asbury, Trisha N. Davis and Linda Wordeman 32. In vitro assays to study the tracking of shortening microtubule ends and to measure associated forces Ekaterina L. Grishchuk and Fazly I. Ataullakhanov

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詳細情報

• NII書誌ID(NCID): BB02334201
• ISBN:
  • 9780123748157
• 出版国コード: us
• タイトル言語コード: eng
• 本文言語コード: eng
• 出版地: Burlington, Mass.
• ページ数/冊数: xxi, 700 p., [32] p. of plates
• 大きさ: 26 cm
• 分類:
  • DC22 : 571.654
• 件名:
  • LCSH : Microtubules
• 親書誌ID:
  • BA00653438