Protocols for adult stem cells
著者
書誌事項
Protocols for adult stem cells
(Methods in molecular biology / John M. Walker, series editor, 621)(Springer protocols)
Humana , Springer, c2010
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
Although embryonic stem cells currently enjoy the public limelight and show great pr- ise for cell based medical therapies, it is the adult stem cells which are responsible for the body's natural ability to fght disease, heal and recover, or fail and succumb to various maladies. The study of mammalian adult stem cells has surged recently, most likely from a maturation of stem cell studies in the classical developmental model organisms and in hematopoeisis. All the tissues of the body examined so far are generated and regenerated from stem cells, it has been an important frst step to adapt or devise new methods to identify and obtain these cells in quantity and purity for further study. Culture techniques have been optimized for managing the growth and differentiation of stem cells in vitro; as some stem cells are pluripotent, often the method is to guide the fate of such cells among the possible differentiation fates. Much of this work, and that in the classical model org- isms, has helped defne the aspects of the stem cell environment or niche that are crucial for both growth and differentiation, and these studies have moved in vivo at increasingly higher resolution. Importantly, the in vivo niche is a current target for bioengineering the matrix and signaling factors. Herein, we present methods for studying six types of mammalian stem cells, m- mary, neural, mesenchymal, endothelial, dendritic, and muscle.
目次
Protocols for Adult Stem Cells
Editors Irina Conboy, David Schaffer, Mary Barcellos-Hoff, and Song Li
Table of Contents:
In Situ Analysis of Cell Populations: Long-Term Label-Retaining Cells
Rodrigo Fernandez-Gonzalez, Irineu Illa-Bochaca, Dawne N. Shelton,
Bryan E. Welm , Carlos Ortiz-deSolorzano and Mary Helen Barcellos-Hoff
Limiting-Dilution Transplantation Assays in Mammary Stem Cell Studies
Rodrigo Fernandez-Gonzalez, Irineu Illa-Bochaca1, Dawne N. Shelton,
Bryan E. Welm , Carlos Ortiz-deSolorzano and Mary Helen Barcellos-Hoff
Use of Stem Cell Markers in Dissociated Mammary Populations
Rodrigo Fernandez-Gonzalez,Irineu Illa-Bochaca, Dawne N. Shelton,
Bryan E. Welm, Carlos Ortiz-deSolorzano and Mary Helen Barcellos-Hoff
Isolation of Adult Hippocampal Neural Progenitors
Joseph Peltier, Brandi K. Ormerod, David V. Schaffer
In vitro Culture and Analysis of Adult Hippocampal Neural Progenitors
Joseph Peltier, Smita Agrawal, Matthew J. Robertson, David V. Schaffer
In vivo Analysis of Engrafted Adult Hippocampal Neural Progenitors
Matthew J. Robertson, Joseph Peltier, David V. Schaffer
Viral Packaging and Transduction of Adult Hippocampal Neural Progenitors
Joseph Peltier, David V. Schaffer
Culture of Bone Marrow Mesenchymal Stem Cells on Engineered Matrix
Jennifer S. Park, Craig Hashi and Song Li
Preparation and analysis of endothelial progenitor cells associated with angiogenesis
Steve Khachi, Yiqian Zhu, and Guo-Yuan Yang
Preparation of adult muscle fiber associated stem/precursor cells
Michael J. Conboy and Irina M. Conboy
Immuno-analysis and FACS sorting of adult muscle fiber associated stem/precursor cells
Michael J. Conboy, Massimiliano Cerletti, Amy J. Wagers and Irina M. Conboy
Altered expression of NF-kappa B in ex vivo differentiated dendritic cells from the aged subjects: Implications in immunotherapy
Anshu Agrawal
Preparation of collagen-coated gels that maximize in vitro myogenesis of stem cells by matching the lateral elasticity of in vivo muscle
Tathagata Chaudhuri, Florian Rehfeldt, H. Lee Sweeney and Dennis E. Discher
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