Single particle tracking and single molecule energy transfer
著者
書誌事項
Single particle tracking and single molecule energy transfer
Wiley-VCH, c2010
大学図書館所蔵 全2件
  青森
  岩手
  宮城
  秋田
  山形
  福島
  茨城
  栃木
  群馬
  埼玉
  千葉
  東京
  神奈川
  新潟
  富山
  石川
  福井
  山梨
  長野
  岐阜
  静岡
  愛知
  三重
  滋賀
  京都
  大阪
  兵庫
  奈良
  和歌山
  鳥取
  島根
  岡山
  広島
  山口
  徳島
  香川
  愛媛
  高知
  福岡
  佐賀
  長崎
  熊本
  大分
  宮崎
  鹿児島
  沖縄
  韓国
  中国
  タイ
  イギリス
  ドイツ
  スイス
  フランス
  ベルギー
  オランダ
  スウェーデン
  ノルウェー
  アメリカ
注記
Includes bibliographical references and index
内容説明・目次
内容説明
Closing a gap in the literature, this handbook gathers all the information on single particle tracking and single molecule energy transfer. It covers all aspects of this hot and modern topic, from detecting virus entry to membrane diffusion, and from protein folding using spFRET to coupled dye systems, as well recent achievements in the field. Throughout, the first-class editors and top international authors present content of the highest quality, making this a must-have for physical chemists, spectroscopists, molecular physicists and biochemists.
目次
Preface
PART I: Single-Particle Imaging and Tracking
THREE DIMENSIONAL PARTICLE TRACKING IN A LASER SCANNING FLUORESCENCE MICROSCOPE
Introduction
Image-Based Single-Particle Tracking Methods
Advanced Fluorescence Microscopy Techniques for Single-Particle Tracking
Two-Photon Excitation Microscopy
3-D Tracking in Image-Based SPT Approaches
3-D Tracking in Laser Scanning Microscopes
Instrumentation
Background Noise
Simultaneous Two-Particle Tracking
Application: Chromatin Dynamics in Interphase Cells
Conclusions
THE TRACKING OF INDIVIDUAL MOLECULES IN CELLS AND TISSUES
Introduction
Single-Molecule and Single-Particle Localization
Positional Accuracy
Tracking
Trajectory Analysis
Applications
Conclusions
MESSENGER RNA TRAFFICKING IN LIVING CELLS
Intranuclear Structure and Dynamics
FCS and FRAP Studies of Nuclear mRNP Mobility
Single-Particle Tracking of mRNA Molecules
Single-Particle Tracking of Specific, Native mRNPs
In Vivo Labeling of Native BR2 mRNPs
Outlook: Light Sheet-Based Single-Molecule Microscopy
QUANTUM DOTS: INORGANIC FLUORESCENT PROBES FOR SINGLE-MOLECULE TRACKING EXPERIMENTS IN LIVE CELLS
Introduction
Fluorescent Labels for Single-Molecule Tracking in Cells
Optical Properties of Colloidal Quantum Dots
Synthesis of Colloidal Fluorescent Quantum Dots
Surface Chemistry for the Water-Solubilization of Quantum Dots
Interfacing Quantum Dots with Biology
Single Quantum Dot Tracking Experiments in Live Cells
Conclusions and Perspectives
PART II: Energy Transfer on the Nanoscale
SINGLE-PAIR FRET: AN OVERVIEW WITH RECENT APPLICATIONS AND FUTURE PERSPECTIVES
Introduction
Principles of FRET
spFRET in Solution
spFRET on Immobilized Molecules
Future Prospects
ALTERNATING-LASER EXCITATION AND PULSED-INTERLEAVED EXCITATION OF SINGLE MOLECULES
Introduction
ALEX: The Principles of Operation
MyALEX
Nanosecond-ALEX/Pulsed Interleaved Excitation (PIE)
msALEX
Three-Color ALEX
Conclusions and Outlook
UNRAVELING THE DYNAMICS BRDIGING PROTEIN STRUCTURE AND FUNCTION ONE MOLECULE AT A TIME
Introduction
Converting Chemical Energy to Mechanical Work: Molecular Motors
Allostery in Proteins
Enzyme Catalysis
Conclusions
QUANTITATIVE DISTANCE AND POSITION MEASUREMENT USING SINGLE-MOLECULE FRET
Introduction
Fundamentals of FRET
FRET as a Spectroscopic Ruler: Initial Experiments and Limitations
Measuring the Quantum Yield
The Orientation of Donor and Acceptor Molecules
Accurate FRET Measurements Using Fluorescence Correlation Spectroscopy
FRET-Based Triangulation and the Nanopositioning System
Conclusions and Outlook
PART III: Single Molecules in Nanosystems
COHERENT AND INCOHERENT COUPLING BETWEEN A SINGLE DIPOLAR EMITTER AND ITS NANOENVIRONMENT
Introduction
Systems
Coupling of Two Oscillating Dipoles
A Dipole Close to a Surface
A Single Molecule and a Single Nanoparticle
Modification of the Spontaneous Emission and Quantum Efficiency by Nanoantennae
Conclusions
ENERGY TRANSFER IN SINGLE CONJUGATED POLYMER CHAINS
Introduction
Why Single Chain Spectroscopy?
Experimental Approach and Material Systems
Photophysics of Single Conjugated Polymer Chains
Energy Transfer in Single Chains
Influence of Initial Excitation Energy on Energy Transfer
Conclusions
REACTIONS AT THE SINGLE-MOLECULE LEVEL
Introduction
Biocatalysis at the Single-Molecule Level
Chemocatalysis at the Single-Molecule Level
VISUALIZING SINGLE-MOLECULE DIFFUSION IN NANOCHANNEL SYSTEMS
Introduction
Correlation of Structural and Dynamic Properties Using TEM and SMT
Phase Mixture
Heterogeneous Dynamics of a Single Molecule
Oriented Single Molecules with Switchable Mobility in Long Unidimensional Nanochannels
High Localization Accuracy of Single Molecules Down to the Single Channel Limit
Probing Chemical Interactions in Silica Thin Films Using Fluorescence Correlation Spectroscopy (FCS)
Functionalized Mesoporous Silica Structures
Single-Molecule Studies of Mesoporous Silica Structures for Drug-Delivery Applications
Conclusions and Outlook
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