Mouse molecular genetics
著者
書誌事項
Mouse molecular genetics
(Methods in enzymology / editors in chief, Sidney P. Colowick, Nathan O. Kaplan, v. 477 . Guide to techniques in mouse development ; pt. B)
Academic Press, 2010
2nd ed
- : hardback
- : pbk
大学図書館所蔵 全41件
  青森
  岩手
  宮城
  秋田
  山形
  福島
  茨城
  栃木
  群馬
  埼玉
  千葉
  東京
  神奈川
  新潟
  富山
  石川
  福井
  山梨
  長野
  岐阜
  静岡
  愛知
  三重
  滋賀
  京都
  大阪
  兵庫
  奈良
  和歌山
  鳥取
  島根
  岡山
  広島
  山口
  徳島
  香川
  愛媛
  高知
  福岡
  佐賀
  長崎
  熊本
  大分
  宮崎
  鹿児島
  沖縄
  韓国
  中国
  タイ
  イギリス
  ドイツ
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  フランス
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  オランダ
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注記
Includes bibliographical references and indexes
内容説明・目次
- 巻冊次
-
: hardback ISBN 9780123848802
内容説明
This volume comprehensively covers new technologies and methodologies that have appeared for the study of mouse development
目次
Section I. Transgenesis 1. Lentivirus transgenesis Alexander Pfeifer and Katrin Zimmermann 2. Germline modification using mouse spermatogonial stem cells Mito Kanatsu-Shinohara and Takashi Shinohara 3. Embryonic in vivo electroporation in the mouse Tetsuichiro Saito Section II. Transposons 4. Current applications of transposons in mouse genetics Adam J. Dupuy 5. Functional genomics in the mouse using the Sleeping Beauty transposon system Kyoji Horie, Chikara Kokubu, and Junji Takeda 6. The use of DNA transposons for cancer gene discovery in mice George Vassiliou, Roland Rad, and Allan Bradley Section III. Recombinases 7. A practical summary of site specific recombination, conditional mutagenesis, and tamoxifen induction of CreERT2 Konstantinos Anastassiadis, Stefan Glaser, Andrea Kranz, Kaj Berhardt, and A. Francis Stewart 8. A recombineering pipeline to make conditional targeting constructs Jun Fu, Madeleine Teucher, Konstantinos Anastassiadis, William Skarnes, and A. Francis Stewart 9. Confirmation of recombination site functionality in gene targeting vectors using recombinase-expressing bacteria M. David Stewart and Richard R. Behringer 10.Genetic fate mapping using site-specific recombinases Emilie Legue and Alexandra L. Joyner 11. Mapping cell fate and function using recombinase-based intersectional strategies Susan M. Dymecki, Russell S. Ray, and Jun C. Kim Section IV. Mutagenesis 12. Genome-wide forward genetic screens in mouse ES cells Meng Amy Li, Stephen J. Pettitt, Kosuke Yusa, and Allan Bradley 13. Gene trap mutagenesis in the mouse Roland H. Friedel and Philippe Soriano 14. A wider context for gene trap mutagenesis Joshua M. Brickman, Anestis Tsakiridis, Christine To, and William L. Stanford 15. Mouse mutagenesis with the chemical supermutagen ENU Frank J. Probst and Monica J. Justice 16. Phenotype-driven mouse ENU mutagenesis screens Tamara Caspary 17. Using ENU mutagenesis for phenotype-driven analysis of the mouse. Rolf W. Stottmann and David R. Beier Section V. Gene Knockdowns 18. Exploration of self-renewal and pluripotency in ES cells using RNAi Christoph Schaniel, Dung-Fang Lee, and Ihor R. Lemischka 19. Transgenic RNAi applications in the mouse Jost Seibler and Frieder Schwenk 20. Gene knockdown in the mouse through RNAi Aljoscha Kleinhammer, Wolfgang Wurst, and Ralf Kuhn 21. In vivo analysis of gene knock-down in tetracycline-inducible shRNA mice Christopher S. Raymond, Lei Zhu, and Myung K. Shin 22. The power of reversibility: Regulating gene activities via tetracycline controlled transcription Kai Schoenig, Hermann Bujard, and Manfred Gossen Section VI. Gene Expression Profiling 23. Gene expression profiling of mouse oocytes and preimplantation embryos Francesca E. Duncan and Richard M. Schultz 24. Interrogating the transcriptome of oocytes and preimplantation embryos Anne E. Peaston, Joel Graber, Barbara B. Knowles, and Wilhelmine N. de Vries 25. Gene expression profiling of mouse embryos with microarrays Alexei A. Sharov, Yulan Piao, and Minoru S. H. Ko
- 巻冊次
-
: pbk ISBN 9780123848826
内容説明
Guide to Techniques in Mouse Development, Part B, is an authoritative guide to different methods used in enzymology, focusing on investigating mouse development using technological advances. The text provides information regarding the principles of the methods in mouse development, and it offers readers reliable experimental protocols and recipes described comprehensively by leaders in the field of enzymology.
The text is divided into three sections and organized into 25 chapters. Below are several concepts covered by the text:
Lentivirus transgenesis o Germline modification using mouse stem cells
Electroporation
Applications of transposons in mouse genetics
Functional genomics using transposon systems
The use of DNA transposons in detecting cancer genes in mice
Recombination, conditional mutagenesis and induction of tamoxifen
Genetic fate mapping using recombinases
Genetic screens mouse ES cells
Gene trap mutagenesis
Mouse mutagenesis
Self- renewal and pluripotency
Transgenic RNAi applications
Gene knockdowns
Tetracycline-controlled transcription
Gene expression profiling of mouse embryos
The book is a comprehensive guide for students and professionals in genetics, cytology and molecular biology, who will find this book invaluable for their learning and practice.
目次
Section I. Transgenesis1. Lentivirus transgenesis2. Germline modification using mouse spermatogonial stem cells3. Embryonic in vivo electroporation in the mouse
Section II. Transposons4. Current applications of transposons in mouse genetics5. Functional genomics in the mouse using the Sleeping Beauty transposon system6. The use of DNA transposons for cancer gene discovery in mice
Section III. Recombinases7. A practical summary of site specific recombination, conditional mutagenesis, and tamoxifen induction of CreERT28. A recombineering pipeline to make conditional targeting constructs 9. Confirmation of recombination site functionality in gene targeting vectors using recombinase-expressing bacteria10.Genetic fate mapping using site-specific recombinases11. Mapping cell fate and function using recombinase-based intersectional strategies
Section IV. Mutagenesis12. Genome-wide forward genetic screens in mouse ES cells13. Gene trap mutagenesis in the mouse14. A wider context for gene trap mutagenesis 15. Mouse mutagenesis with the chemical supermutagen ENU16. Phenotype-driven mouse ENU mutagenesis screens17. Using ENU mutagenesis for phenotype-driven analysis of the mouse
Section V. Gene Knockdowns18. Exploration of self-renewal and pluripotency in ES cells using RNAi19. Transgenic RNAi applications in the mouse 20. Gene knockdown in the mouse through RNAi21. In vivo analysis of gene knock-down in tetracycline-inducible shRNA mice22. The power of reversibility: Regulating gene activities via tetracycline controlled transcription
Section VI. Gene Expression Profiling23. Gene expression profiling of mouse oocytes and preimplantation embryos24. Interrogating the transcriptome of oocytes and preimplantation embryos25. Gene expression profiling of mouse embryos with microarrays
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