Plant secondary metabolism engineering : methods and applications
著者
書誌事項
Plant secondary metabolism engineering : methods and applications
（Methods in molecular biology / John M. Walker, series editor, 643）（Springer protocols）
Humana, c2010
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
Plants have evolved an amazing array of metabolic pathways leading to molecules capable of responding promptly and effectively to stress situations imposed by biotic and abiotic factors, some of which supply the ever-growing needs of humankind for natural chemicals, such as pharmaceuticals, nutraceuticals, agrochemicals, food and chemical additives, biofuels, and biomass. In Plant Secondary Metabolism Engineering: Methods and Applications, expert researchers provide detailed practical information on some of the most important methods employed in the engineering of plant secondary metabolism pathways and in the acquisition of essential knowledge in performing this activity, including the significant advances and emerging strategies. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Plant Secondary Metabolism Engineering: Methods and Applications will aid scientists engaged in the challenging task of modifying some of the most intricate products of plant evolution and support their efforts directed toward the vital goal of sustainable natural chemicals.
目次
1. Plant Secondary Metabolism and Challenges in Modifying Its Operation: An Overview
Naila Cannes do Nascimento and Arthur Germano Fett-Neto
2. Suppression Subtractive Hybridization as a Tool to Identify Anthocyanin Metabolism-Related Genes in Apple Skin
Yusuke Ban and Takaya Moriguchi
3. Identification of Regulatory Protein Genes Involved in Alkaloid Biosynthesis Using a Transient RNAi System
Yasuyuki Yamada, Nobuhiko Kato, Yasuhisa Kokabu, Qingyun Luo, Joseph Gogo Dubouzet, and Fumihiko Sato
4. Site-Directed Mutagenesis and Saturation Mutagenesis for the Functional Study of Transcription Factors Involved in Plant Secondary Metabolite Biosynthesis
Sitakanta Pattanaik, Joshua R. Werkman, Que Kong, and Ling Yuan
5. Isolation of Proteins Binding to Promoter Elements of Alkaloid Metabolism-Related Genes Using Yeast One-Hybrid
Debora Vom Endt and Giancarlo Pasquali
6. Modulation of Carotenoid Accumulation in Transgenic Potato by Inducing Chromoplast Formation with Enhanced Sink Strength
Joyce Van Eck, Xiangjun Zhou, Shan Lu, and Li Li
7. Overexpression of Rate-Limiting Enzymes to Improve Alkaloid Productivity
Tomoya Takemura, Yit-lai Chow, Takehiko Todokoro, Takuya Okamoto, and Fumihiko Sato
8. Microbial Expression of Alkaloid Biosynthetic Enzymes for Characterization of Their Properties
Hiromichi Minami, Nobuhiro Ikezawa, and Fumihiko Sato
9. Producing a Recombinant Flavin-Containing Monooxygenase from Coffea arabica in Escherichia coli for Screening of Potential Natural Substrates
Igor Cesarino and Paulo Mazzafera
10. Efficient Production of Active Form Recombinant Cassava Hydroxynitrile Lyase Using Escherichia coli in Low-Temperature-Culture
Hisashi Semba, Eita Ichige, Tadayuki Imanaka, Haruyuki Atomi, and Hideki Aoyagi
11. Introduction of theEarly Pathway to Taxol Biosynthesis in Yeast by Means of Biosynthetic Gene Cluster Construction Using SOE-PCR and Homologous Recombination
Pia Dahm and Stefan Jennewein
12. Biocatalytic Synthesis of Tritium (3H) Labelled Taxa-4(5),11(12)-diene, the Pathway Committing Precursor of the Taxoid Diterpenoids
Hans Schmeer and Stefan Jennewein
13. USER Cloning and USER Fusion: The Ideal Cloning Techniques for Small and Big Laboratories
Hussam H. Nour-Eldin, Fernando Geu-Flores, and Barbara A. Halkier
14. Enrichment of Carotenoids in Flaxseed by Introducing a Bacterial Phytoene Synthase Gene
Masaki Fujisawa and Norihiko Misawa
15. Metabolic Engineering by Plastid Transformation as a Strategy to Modulate Isoprenoid Yield in Plants
Tomohisa Hasunuma, Akihiko Kondo, and Chikahiro Miyake
16. Engineering High Yields of Secondary Metabolites in Rubia Cell Cultures through Transformation with Rol Genes
Victor P. Bulgakov, Yuri N. Shkryl, and Galina N. Veremeichik
17. Flow Cytometric Methods to Investigate Culture Heterogeneities for Plant Metabolic Engineering
Vishal Gaurav, Martin E. Kolewe, and Susan C. Roberts
18. Phenylpropanoid Biosynthesis in Leaves and Glandular Trichomes of Basil (Ocimum basilicum L.)
Cicero Deschamps and James E. Simon
19. Fusion with Fluorescent Proteins for Subcellular Localization of Enzymes Involved in Plant Alkaloid Biosynthesis
Patricia Duarte, Johan Memelink, and Mariana Sottomayor
20. Immunohistochemical Localization of a Putative Flavonoid Transporter in Grape Berries
Elisa Petrussa, Enrico Braidot, Marco Zancani, Carlo Peresson, Alberto Bertolini, Sonia Patui, Valentino Casolo, Sabina Passamonti, Francesco Macri, and Angelo Vianello
21. Electrogenic Bromosulfalein Transport in Isolated Membrane Vesicles:
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