Analysis of cells and tissues
Author(s)
Bibliographic Information
Analysis of cells and tissues
（Methods in molecular medicine / John M. Walker, series editor, 57 . Metastasis research protocols ; v. 1）
Humana Press, c2010
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Note
Includes bibliographical references and index
Description and Table of Contents
Description
The process of metastasis formation is hugely complex, as described in the introductory chapter of this book, and this complexity has led us to compile two volumes of methods, from a vastly divergent background that attempts to encompass the whole spectrum of cancer biology. This first volume, Metastasis Research Protocols: Analysis of Cells and Tissues, concentrates on analysis and mapping of molecules produced by cells and tissues and analysis of the molecular biology underlying their expression, whereas the second volume, Metastasis Research Protocols: Cell Behavior In Vitro and In Vivo,focuses sharply on the determination of cell behavior in vitro and in vivo. We have deliberately included chapters describing well-established and familiar te- niques (for example, SDS-PAGE and Western blotting [Chapter 11], and immunocytochemistry [Chapter 2]) in addition to the newer and more speci- ized approaches and specific examples of their application, because-although the methodology is readily available in the published literature and established in many laboratories-we wished these volumes to "stand alone" and to make accessible here the standard techniques that underpin much metastasis research for both the newcomer to the field and the seasoned researcher. Undoubtedly, owing to the complexity of the metastatic cascade and the wealth of research techniques involved in scientific approaches to its unraveling, and despite our best efforts to make these volumes as comprehensive as seems feasible, this is a tall order, and there will inevitably be omissions. For these we apologize.
Table of Contents
Part I. Detection Methods for Cellular Markers of Metastatic Potential
Histopathological Assessment of Metastasis
Derek E. Roskell and Ian D. Buley
Basic Immunocytochemistry for Light Microscopy
Susan A. Brooks
Multiple Labeling Techniques for Fluorescence Microscopy
Amanda J. Atherton and Catherine Clarke
Lectin Histochemistry to Detect Altered Glycosylation in Cells and Tissues
Susan A. Brooks and Debbie M. S. Hall
Immunocytochemical Detection and Characterization of Individual Micrometastatic Tumor Cells
Stephan Braun and Klaus Pantel
Understanding Metastasis Through Integrin Expression
Lawrence John and Massimo Pignatelli
Surrogate Markers of Angiogenesis and Metastasis
Girolamo Ranieri and Giampietro Gasparini
Measurement of Microvessel Density in Primary Tumors
Helen E. Turner and Adrian L. Harris
Assessment of Cellular Proliferation by Calculation of Mitotic Index and by Immunohistochemistry
Cheryl E. Gillett
Flow Cytometric Measurement of Cell Proliferation
Richard S. Camplejohn
SDS-PAGE and Western Blotting Techniques
Christine Blancher and Adam Jones
Assessment of Gelatinases (MMP-2 and MMP-9) by Gelatin Zymography
Marta Toth and Rafael Fridman
Part II. Genetic Aspects of Metastasis
In Situ Hybridization to Localize mRNAs
Richard Poulsom
Fluorescence In Situ Hybridization
Hakan Goker and Janet Shipley
Comparative Genomic Hybridization
Rebecca Roylance
RNA Purification and Quantification Methods
Adam Jones, Chisato Fujiyama, and Kenneth Smith
Northern and Southern Blotting and the Polymerase Chain Reaction to Detect Gene Expression
Thelma Tennant, Marina Chekmareva, and Carrie W. Rinker-Schaeffer
Methylation Analysis of CpG Islands
Richard Lilischkis, Hermann Kneitz, and Hans Kreipe
Detection of Metastatic Tumor Cells in Blood by Reverse Transcriptase-Polymerase Chain Reaction
Katja Haack, Krishna Soondrum, and Michael Neumaier
Differential Display
Ruey Ho Kao and Giulio Francia
Part III. Mathematical Modeling of Metastases
Predictive Mathematical Modeling in Metastasis
Jonathan A. Sherratt
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