Photosynthesis research protocols

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書誌事項

Photosynthesis research protocols

edited by Robert Carpentier

（Springer protocols）（Methods in molecular biology / John M. Walker, series editor, 684）

Humana Press, c2011

2nd ed

  • : hbk

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注記

Includes bibliographical references and index

内容説明・目次

内容説明

The capture of sunlight by photosynthetic organisms supplies an enormous amount of the energy required to develop and sustain life on the planet, making photosynthesis one of the most important biological phenomenon on Earth. Updating the popular first edition, Photosynthesis Research Protocols, Second Edition presents detailed descriptions of a broad range of general and fundamental methods that are commonly used by plant biochemists, physiologists, and molecular biologists, all of which are contributed by leading researchers in the field. This vital new edition also features coverage of methods related to the most abundant protein on earth, Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase. Written in the highly successful Methods in Molecular Biology (TM) series format, the chapters of this volume include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Photosynthesis Research Protocols, Second Edition serves new photosynthesis researchers as well as experienced scientists seeking to use a new type of preparation or method to study this tremendously influential process.

目次

1. Isolation of Photosystem II-Enriched Membranes and the Oxygen-Evolving Complex (OEC) Subunit Proteins from Higher Plants Yasusi Yamamoto, Jing Leng, and Jian-Ren Shen 2. Isolation of Photosystem I Submembrane Fractions Johanne Harnois, Najoua Msilini, and Robert Carpentier 3. Isolation of Photosystem II Reaction Center Complexes from Plants Michael Seibert and Rafael Picorel 4. Methods for the Isolation of Functional Photosystem II Core Particles from the Cyanobacterium Synechocystis sp. PCC 6803 Dmitrii V. Vavilin 5. Purification and Crystallization of Oxygen-Evolving Photosystem II Core Complex from Thermophilic Cyanobacteria Jian-Ren Shen, Keisuke Kawakami, and Hiroyuki Koike 6. Isolation of Cytochrome b6f Complex from Grana and Stroma Membranes from Spinach Chloroplasts Elzbieta Romanowska 7. Purification and Crystallization of the Cytochrome b6f Complex Danas Baniulis, Huamin Zhang, Taisaya Zakharova, S. Saif Hasan, and William A. Cramer 8. Purification of Plastocyanin and Cytochrome c6 from Plants, Green Algae, and Cyanobacteria Jose A. Navarro, Manuel Hervas, and Miguel A. De la Rosa 9. Isolation and Identification of Chloroplast Lipids Norihiro Sato and Mikio Tsuzuki 10. Isolation and Purification of CP43 and CP47 Photosystem II Proximal Antenna Complexes from Plants Rafael Picorel, Miguel Alfonso, and Michael Seibert 11. Preparation of Native and Recombinant Light-Harvesting Chlorophyll-a/b Complex Wolfgang Ruhle and Harald Paulsen 12. Isolation and Characterization of Lamellar Aggregates of LHCII and LHCII-Lipid Macro-Assemblies with Light-Inducible Structural Transitions Ilian Simidjiev, Zsuzsanna Varkonyi, Petar H. Lambrev, and Gyozo Garab 13. Protein Targeting across and into Chloroplast Membranes Steven M. Theg and Shari M. Lo 14. Proteomic Analysis of Thylakoid Membranes Venkateswarlu Yadavalli, Sreedhar Nellaepalli, and Rajagopal Subramanyam 15. Thylakoid Phosphoproteins: Identification of Phosphorylation Sites Anne Rokka, Eva-Mari Aro, and Alexander V. Vener 16. Direct Detection of Free Radicals and Reactive Oxygen Species in Thylakoids Eva Hideg, Tamas Kalai, and Kalman Hideg 17. Assay of Photoinhibition and Heat Inhibition of Photosystem II in Higher Plants Nobuyoshi Nijo, Bjoern Lundin, Miho Yoshioka, Noriko Morita, and Yasusi Yamamoto 18. Photosystem II Reconstitution into Proteoliposomes and Methodologies for Structure-Function Characterization David Joly, Sridharan Govindachary, and Mario Fragata 19. Physical and Chemical Immobilization Methods of Photosynthetic Materials Lise Barthelmebs, Robert Carpentier, and Regis Rouillon 20. Identifying Chloroplast Biogenesis and Signalling Mutants in Arabidopsis thaliana Veronica Albrecht, Gonzalo M. Estavillo, Abby J. Cuttriss, and Barry J. Pogson 21. Expression of Genes in Cyanobacteria: Adaptation of Endogenous Plasmids as Platforms for High-Level Gene Expression in Synechococcus sp. PCC 7002 Yu Xu, Richard M. Alvey, Patrick O. Byrne, Joel E. Graham, Gaozhong Shen, and Donald A. Bryant 22. Construction of Gene Interruptions and Gene Deletions in the Cyanobacterium Synechocystis sp. Strain PCC 6803 Julian J. Eaton-Rye 23. A Simple Method for Chloroplast Transformation in Chlamydomonas reinhardtii Vellupillai M. Ramesh, Scott E. Bingham, and Andrew N. Webber 24. Rapid Isolation of Intact Chloroplasts from Spinach Leaves David Joly and Robert Carpentier 25. Mechanical Isolation of Bundle Sheath Cell Strands and Thylakoids from Leaves of C4 Grasses Elzbieta Romanowska and Eugeniusz Parys 26. Isolation of Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase from Leaves A. Elizabete Carmo-Silva, Csengele Barta, and Michael E. Salvucci 27. Quantifying the Amount and Activity of Rubisco in Leaves David S. Kubien, Christopher M. Brown, and Heather J. Kane 28. Purification of Rubisco Activase from Leaves or after Expression in Escherichia coli Csengele Barta, A. Elizabete Carmo-Silva, and Michael E. Salvucci 29. Rubisco Activase Activity Assays Csengele Barta, A. Elizabete Carmo-Silva, and Michael E. Salvucci 30. Quantification of Rubisco Activase Content in Leaf Extracts Wataru Yamori and Susanne von Caemmerer

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