Engineered zinc finger proteins : methods and protocols
著者
書誌事項
Engineered zinc finger proteins : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, 649）（Springer protocols）
Humana Press, c2010
大学図書館所蔵 件 / 全2件
-
該当する所蔵館はありません
- すべての絞り込み条件を解除する
注記
Includes bibliographical references and index
内容説明・目次
内容説明
Among the many types of DNA binding domains, C2H2 zinc finger proteins (ZFPs) have proven to be the most malleable for creating custom DNA-binding proteins. In Engineered Zinc Finger Proteins: Methods and Protocols, expert researchers from some of the most active laboratories in this field present detailed methods, guidance, and perspectives. The volume contains sections covering the engineering of ZFPs, methods for the creation, evaluation, and delivery of artificial transcription factors (ATFs), methods for the creation and evaluation of zinc finger nucleases (ZFNs), and a collection of the several applications and assays beyond ATFs and ZFNs, including zinc finger transposases and ChIP-seq methodology amongst other subjects. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Comprehensive and cutting-edge, Engineered Zinc Finger Proteins: Methods and Protocols aims to aid both seasoned practitioners and new investigators with its vital methods and insights as they seek to create the next generation of engineered ZFPs and applications.
目次
Part I: Engineering Zinc Finger Proteins
1. The Generation of Zinc Finger Proteins by Modular Assembly
Mital S. Bhakta and David J. Segal
2. Engineering Single Cys2His2 Zinc Finger Domains using a Bacterial Cell-Based Two-Hybrid Selection System
Stacey Thibodeau-Beganny, Morgan L. Maeder, and J. Keith Joung
3. Bipartite Selection of Zinc Fingers by Phage Display for Any 9-bp DNA Target Site
Jia-Ching Shieh
4. Structure-Based DNA Binding Prediction and Design
Andreu Alibes, Luis Serrano, and Alejandro D. Nadra
Part II: Artificial Transcription Factors
5. Generation of Cell-Permeable Artificial Zinc Finger Protein Variants
Takashi Sera
6. Inhibition of Viral Transcription Using Designed Zinc Finger Proteins
Kimberley A. Hoeksema and D. Lorne J. Tyrrell
7. Modulation of Gene Expression Using Zinc Finger Based Artificial Transcription Factors
Sabine Stolzenburg, Alan Bilsland, W. Nicol Keith, and Marianne G. Rots
8. Construction of Combinatorial Libraries that Encode Zinc Finger-Based Transcription Factors
Seokjoong Kim, Eun Ji Kim, and Jin-Soo Kim
9. Silencing of Gene Expression by Targeted DNA Methylation: Concepts and Approaches
Renata Z. Jurkowska and Albert Jeltsch
10. Remodeling Genomes with Artificial Transcription Factors (ATFs)
Adriana S. Beltran and Pilar Blancafort
11. Transgenic Mice Expressing an Artificial Zinc Finger Regulator Targeting an Endogenous Gene
Claudio Passananti, Nicoletta Corbi, Annalisa Onori, Maria Grazia Di Certo, and Elisabetta Mattei
Part III: Zinc Finger Nucleases
12. Artificial Zinc Finger Nucleases for DNA Cloning
Vardit Zeevi, Andriy Tovkach, and Tzvi Tzfira
13. In Vitro Assessment of Zinc-Finger Nuclease Activity
Toni Cathomen and Cem Soellu
14. Assessing Zinc Finger Nuclease-Associated Toxicity
Tatjana I. Cornu and Toni Cathomen
15. A Rapid and General Assay for Monitoring Endogenous Gene Modification
Dmitry Y. Guschin, Adam J. Waite, George E. Katibah, Jeffrey C. Miller, Michael C. Holmes, andEdward J. Rebar
16. Engineered Zinc Finger Proteins for Manipulation of the Human Mitochondrial Genome
Michal Minczuk
17. High Efficiency Gene Targeting in Drosophila with Zinc Finger Nucleases
Dana Carroll, Kelly J. Beumer, and Jonathan K. Trautman
18. Using Zinc Finger Nucleases for Efficient and Heritable Gene Disruption in Zebrafish
Jasmine M. McCammon and Sharon L. Amacher
19. A Transient Assay for Monitoring Zinc Finger Nuclease Activity at Endogenous Plant Gene Targets
Justin P. Hoshaw, Erica Unger-Wallace, Feng Zhang, and Daniel F. Voytas
20. Validation and Expression of Zinc Finger Nucleases in Plant Cells
Andriy Tovkach, Vardit Zeevi, and Tzvi Tzfira
21. Non-FokI-Based Zinc Finger Nucleases
Miki Imanishi, Shigeru Negi, and Yukio Sugiura
Part IV: Beyond Transcription and Cleavage
22. Designing and Testing Chimeric Zinc Finger Transposases
Matthew H. Wilson and Alfred L. George, Jr.
23. Seeing Genetic and Epigenetic Information without DNA Denaturation Using Sequence Enabled Reassembly (SEER)
Jason R. Porter, Sarah H. Lockwood, Mital S. Bhakta, David J. Segal, and Indraneel Ghosh
24. Zinc Finger-Mediated Live Cell Imaging in Arabidopsis Roots
Beatrice I. Lindhout, Tobias Meckel, and Bert J. van der Zaal
25. Biophysical Analysis of the Interaction of Toxic Metal Ions and Oxidants with the Zinc Finger Domain of XPA
Andrea Hartwig, Tanja Schwerdtle, and Wojciech Bal
26. Preparation and Zinc-Binding Properties of Multi-Fingered Zinc-Sensing Domains
John H. Laity and Linda S. Feng
27. Using ChIP-seq Technology to Identify Targets of Zinc Finger Transcription Factors
Henriette O'Geen, Seth Frietze, and Peggy J. Farnham
28. Crystallization of Zinc Finger Proteins Bound to DNA
Nancy C. Horton and Chad K. Park
29. Beyond DNA: Zinc Finger Domains as RNA-Binding Modules
Josep Font and Joel P. Mackay
「Nielsen BookData」 より
