Quorum sensing : methods and protocols
著者
書誌事項
Quorum sensing : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, 692）（Springer protocols）
Humana Press, c2011
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
Since its early days in the 1990s, the Quorum Sensing (QS) field has grown from a few dozen laboratories, investigating the pathways, proteins, and chemicals that facilitate signaling in bacteria, to hundreds of groups that have integrated evolutionary biology, computer science, mathematics, engineering, and metagenomics to create an ever-expanding and dynamic field. In Quorum Sensing: Methods and Protocols, expert researchers provide an in-depth set of diverse protocols that span this broad area of study. Broken into three detailed sections, the volume covers the detection, isolation, and characterization of the QS signals made by both Gram- and Gram+ bacteria, determination of the function of QS signals in vivo, and the development of QS disruption strategies. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips on troubleshooting and avoiding known experimental pitfalls.
Comprehensive and cutting-edge, Quorum Sensing: Methods and Protocols serves as an invaluable collection of easily accessible techniques for scientists seeking to further our knowledge about bacterial communication and its relation to humanity.
目次
Foreword I
by E. Peter Greenberg
Foreword II
by Paul Williams
Part I: Detection, Isolation, and Characterization of Quorum Sensing Compounds
1. Bioassays of Quorum Sensing Compounds Using Agrobacterium tumefaciens and Chromobacterium violaceum
Weihua Chu, Dhiraj A. Vattem, Vatsala Maitin, Mary B. Barnes, and Robert J.C. McLean
2. Detection of 2-alkyl-4-quinolones Using Biosensors
Stephen P. Diggle, Matthew P. Fletcher, Miguel Camara, and Paul Williams
3. FRET-Based Biosensors for the Detection and Quantification of AI-2 Class of Quorum Sensing Compounds
Sathish Rajamani and Richard Sayre
4. Isolation of agr Quorum Sensing Autoinducers
Naomi Balaban
5. Liquid Chromatography/Mass Spectrometry (LC/MS) for the Detection and Quantification of N-acyl-L-homoserine Lactones (AHLs) and 4-hydroxy-2-alkylquinolines (HAQs)
Francois Lepine and Eric Deziel
6. Detection of Autoinducer (AI-2) like Activity in Food Samples
Kirthiram K Sivakumar, Palmy R. Jesudhasan, and Suresh D. Pillai
7. Detection of Bacterial Signaling Molecules in Liquid or Gaseous Environments
Peter Edmonson, Desmond Stubbs, and William Hunt
8. Determination of acyl Homoserine Lactone and Tetramic Acid Concentrations in Biological Samples
Colin A. Lowery, Gunnar F. Kaufmann, and Kim D. Janda
9. Luminescent Reporters and Their Applications for the Characterization of Signals and Signal-Mimics that Alter LasR-Mediated Quorum Sensing
Ali Alagely, Sathish Rajamani, and Max Teplitski
Part II: Determining the Function of Autoinducers In vivo
10. Modulation of Mammalian Cell Processes by Bacterial Quorum Sensing Molecules
Vladimir V. Kravchenko, Richard J. Ulevitch, and Gunnar F. Kaufmann
11. Imaging N-Acyl Homoserine Lactone Quorum Sensing In vivo
Louise Dahl Christensen, Maria van Gennip, Tim Holm Jakobsen, Michael Givskov, and Thomas Bjarnsholt
12. Defining the Structure and Function of Acyl-Homoserine Lactone Autoinducers
Mair E.A. Churchill, Hiruy M. Sibhatu, and Charis L. Uhlson
13. Global Expression Analysis of Quorum-Sensing Controlled Genes
Martin Schuster
14. Small RNA Target Genes and Regulatory Connections in the Vibrio cholerae Quorum Sensing System
Brian K. Hammer and Sine Lo Svenningsen
15. Quantifying Pseudomonas aeruginosa Quinolones and Examining Their Interactions with Lipids
Gregory C. Palmer, Jeffrey W. Schertzer, Lauren Mashburn-Warren, and Marvin Whiteley
16. Linking Quorum Sensing Regulation and Biofilm Formation by Candida albicans
Aurelie Deveau and Deborah A. Hogan
17. Design of Synthetic Mammalian Quorum-Sensing Systems
Wilfried Weber and Martin Fussenegger
Part III: Quorum Sensing Disruption Strategies
18. Qualitative and Quantitative Determination of Quorum Sensing Inhibition In vitro
Tim Holm Jakobsen, Maria van Gennip, Louise Dahl Christensen, Thomas Bjarnsholt, and Michael Givskov
19. Custom Synthesis of Autoinducers and Their Analogues
Jun Igarashi and Hiroaki Suga
20. Heterologous Overexpression, Purification and in Vitro Characterization of AHL Lactonases
Pei W. Thomas and Walter Fast
21. High Performance Liquid Chromatography Analysis of N-Acyl Homoserine Lactone Hydrolysis by Paraoxonases
John F. Teiber and Dragomir I. Draganov
22. Generation of Quorum Quenching Antibodies
Gunnar F. Kaufmann, Junguk Park, Alexander V. Mayorov, Diane M. Kubitz, and Kim D. Janda
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