Embryonic stem cell therapy for osteo-degenerative diseases : methods and protocols
著者
書誌事項
Embryonic stem cell therapy for osteo-degenerative diseases : methods and protocols
(Methods in molecular biology / John M. Walker, series editor, 690)(Springer protocols)
Humana, c2011
大学図書館所蔵 件 / 全1件
-
該当する所蔵館はありません
- すべての絞り込み条件を解除する
注記
Includes bibliographical references and index
内容説明・目次
内容説明
Embryonic stem cells (ESCs) offer an unlimited self-renewing capacity, as opposed to the limits of adult stem cells; therefore, ESCs represent an almost bottomless resource for regenerative medicine and tissue engineering approaches. In Embryonic Stem Cell Therapy for Osteo-Degenerative Diseases: Methods and Protocols, accomplished investigators provide detailed descriptions on how to expand ESCs from the most commonly used species ex vivo, i.e. mouse and human, in static culture as well as in controllable bioreactor processes. The thorough and timely volume summarizes the methods that may be used to differentiate these cells along the desired lineage of choice, be it osteoblasts, osteoclasts, or chondrocytes, and consequentially also offers analysis tools for the characterization of resulting cells and evaluation of differentiation effectiveness. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips on troubleshooting and avoiding known pitfalls.
Dependable and cutting-edge, Embryonic Stem Cell Therapy for Osteo-Degenerative Diseases: Methods and Protocols supplies the tools necessary to allow researchers to carry out critical research needed in order to bring this burgeoning and vitally important field closer to the clinic and to ensure the widespread application of a successful strategy.
目次
1. Embryonic Stem Cells for Osteo-Degenerative Diseases
Nicole I. zur Nieden
2. Methods for Culturing Mouse and Human Embryonic Stem Cells
Sabrina Lin and Prue Talbot
3. Serum-Free and Feeder free Culture Conditions for Human Embryonic Stem Cells
Ludovic Vallier
4. Functional Assays for Human Embryonic Stem Cell Pluripotency
Michael D. O'Connor, Melanie D. Kardel, and Connie J. Eaves
5. Using Cadherin Expression to Assess Spontaneous Differentiation of Embryonic Stem Cells
Helen Spencer, Maria Keramari, and Christopher M. Ward
6. Generation of Human Embryonic Stem Cells Carrying Lineage Specific Reporters
Parinya Noisa, Alai Urrutikoetxea-Uriguen, and Wei Cui
7. Manipulations of MicroRNA in Human Pluripotent Stem Cells and Their Derivatives
Stephanie N. Rushing, Anthony W. Herren, Deborah K Lieu, and Ronald A. Li
8. Large-Scale Expansion of Mouse Embryonic Stem Cells on Microcarriers
Ana M. Fernandes, Maria Margarida Diogo, Claudia Lobato da Silva, and Joaquim M.S. Cabral
9. Embryoid Body Formation: Recent Advances in Automated Bioreactor Technology
Susanne Trettner, Alexander Seeliger, and Nicole I. zur Nieden
10. Methods for Embryoid Body Formation: The Microwell Approach
Dawn P. Spelke, Daniel Ortmann, Ali Khademhosseini, Lino Ferreira, and Jeffrey M. Karp
11. Human Embryonic Stem Cell-Derived Mesenchymal Progenitors: An Overview
Peiman Hematti
12. Derivation of Mesenchymal Stem Cells from Human Embryonic Stem Cells
Andre Choo and Sai Kiang Lim
13. Differentiation of Human Embryonic Stem Cells into Mesenchymal Stem Cells by the "Raclure" Method
Emmanuel N. Olivier and Eric E. Bouhassira
14. Improved Media Compositions for the Differentiation of Embryonic Stem Cells into Osteoblasts and Chondrocytes
Beatrice Kuske, Vuk Savkovic, and Nicole I. zur Nieden
15. Differentiation of Mouse Embryonic Stem Cells in Self-Assembling Peptide Scaffolds
Nuria Mari-Buye and Carlos E. Semino
16. Methods for Investigation of Osteoclastogenesis Using Mouse Embryonic Stem Cells
Motokazu Tsuneto, Toshiyuki Yamane, and Shin-Ichi Hayashi
17. Absorption-Based Assays for the Analysis of Osteogenic and Chondrogenic Yield
Lesley A. Davis, Anke Dienelt, and Nicole I. zur Nieden
18. Identification of Osteoclasts in Culture
Nobuyuki Udagawa, Teruhito Yamashita, Yasuhiro Kobayashi, and Naoyuki Takahashi
19. Analysis of Glycosaminoglycans in Stem Cell Glycomics
Boyangzi Li, Haiying Liu, Zhenqing Zhang, Hope E. Stansfield, Jonathan S. Dordick, and Robert J. Linhardt
20. Drill Hole Defects: Induction, Imaging, and Analysis in the Rodent
Andre Obenaus and Pedro Hayes
21. Measurement and Illustration of Immune Interaction after Stem Cell Transplantation
Stephan Fricke
「Nielsen BookData」 より