Fluorescence in situ hybridization (FISH) : protocols and applications
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Bibliographic Information
Fluorescence in situ hybridization (FISH) : protocols and applications
（Methods in molecular biology / John M. Walker, series editor, 659）（Springer protocols）
Humana Press, c2010
- : hardcover
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Includes bibliographical references and index
Description and Table of Contents
Description
Fluorescence in situ Hybridization (FISH) belongs to that special category of well-established molecular biology techniques that, since their inception a few decades ago, have succeeded in keeping a prominent position within the constantly expanding list of laboratory pro- dures for biomedical research and clinical diagnostics. The design simplicity and cost-effectiveness of the early FISH protocols, combined with the signifcant acceleration of discoveries in related technical areas such as fuor- cence microscopy, digital imaging, and nucleic acid technology have prompted the div- sifcation of the original technique into an outstanding number of imaginative and useful applications, and thus have not only held back its outmoding but have also promoted its expansion into different areas of basic and applied research in the post-genomic era. The 34 chapters included in this book aim at portraying the vibrant complexity and diversity of the current FISH protocol landscape, providing cutting-edge examples of va- ous applications for genetic and developmental research, cancer research, reproductive medicine, diagnostic and prognostic purposes, microbial ecology, and evolutionary st- ies. The book is divided in four parts: (I) Core Techniques, (II) Technical Advancements and Novel Adaptations, (III) Translational FISH: Applications for Human Genetics and Medicine, and (IV) Protocols for Model Organisms.
Table of Contents
Part I: Core Techniques
1. Fluorescence in situ Hybridization (FISH), Basic Principles, and Methodology
Elisa Garimberti and Sabrina Tosi
2. Fluorescence in situ Hybridization on DNA Halo Preparations and Extended Chromatin Fibers
Lauren S. Elcock and Joanna M. Bridger
3. Detection of Nascent RNA Transcripts by Fluorescence in situ Hybridization
Jill M. Brown and Veronica J. Buckle
4. Fluorescence in situ Hybridization Analysis of Formalin Fixed Paraffin Embedded Tissues, Including Tissue Microarrays
Brenda M. Summersgill and Janet M. Shipley
5. Fluorescence in situ Hybridization (FISH) on Tissue Cryosections
Irina Solovei
6. Multiplex Fluorescence in situ Hybridization (M-FISH)
Rhona Anderson
7. Optical Mapping of Protein-DNA Complexes on Chromatin Fibers
Beth Sullivan
8. 3D-FISH on Cultured Cells Combined with Immunostaining
Irina Solovei and Marion Cremer
Part II: Technical Advancements and Novel Adaptations
9. The Comet-FISH Assay for the Analysis of DNA Damage and Repair
Graciela Spivak
10. Direct in situ Hybridization with Oligonucleotide Functionalized Quantum Dot Probes
Laurent A. Bentolila
11. LNA-FISH for Detection of microRNAs in Frozen Sections
Asli N. Silahtaroglu
12. Chromosome Orientation Fluorescence in situ Hybridization (CO-FISH) or Strand-Specific FISH
Susan M. Bailey, Eli S. Williams, Michael N. Cornforth, and Edwin H. Goodwin
13. Combinatorial Oligo FISH: Directed Labeling of Specific Genome Domains in Differentially Fixed Cell Material and Live Cells
Eberhard Schmitt, Jutta Schwarz-Finsterle, Stefan Stei, Carmen Boxler, Patrick Muller, Andriy Mokhir, Roland Kramer, Christoph Cremer, and Michael Hausmann
14. Simultaneous Visualization of FISH Signals and Bromo-deoxyuridine Incorporation by Formamide-Free DNA Denaturation
Daniela Moralli and Zoia L. Monaco
15. CryoFISH: Fluorescence in situ Hybridization on Ultrathin Cryosections
Sheila Q. Xie, Liron-Mark Lavitas, and Ana Pombo
16. Characterization ofChromosomal Rearrangements Using Multicolor-Banding (MCB/m-band)
Thomas Liehr, Anja Weise, Sophie Hinreiner, Hasmik Mkrtchyan, Kristin Mrasek, and Nadezda Kosyakova
17. Visualizing Nucleic Acids in Living Cells by Fluorescence in vivo Hybridization
Joop Wiegant, Anneke K. Brouwer, Hans J. Tanke, and Roeland W. Dirks
Part III: Translational FISH: Applications for Human Genetics and Medicine
18. Quality Control in FISH as Part of a Laboratory's Quality Management System
Ros Hastings
19. FlashFISH: "Same Day" Prenatal Diagnosis of Common Chromosomal Aneuploidies
Sherry Ho and Mahesh A. Choolani
20. FISH for Pre-Implantation Genetic Diagnosis
Paul N. Scriven and Caroline Mackie Ogilvie
21. PNA-FISH on Human Sperm
Franck Pellestor, Cecile Monzo, and Samir Hamamah
22. POD-FISH: A New Technique for Parental Origin Determination Based on Copy Number Variation Polymorphism
Anja Weise, Madeleine Gross, Sophie Hinreiner, Vera Witthuhn, Hasmik Mkrtchyan, and Thomas Liehr
23. Sequence-Based High Resolution Chromosomal Comparative Genomic Hybridization (CGH)
Agata Kowalska, Eva Bozsaky, and Peter F. Ambros
24. ImmunoFISH on Isolated Nuclei from Paraffin-Embedded Biopsy Material
Soo-Yong Tan and Goran Mattsson
25. Fluorescence in situ Hybridization on 3D Cultures of Tumor Cells
Karen J. Meaburn
26. Simultaneous Ultrasensitive Subpopulation Staining/Hybridization in situ (SUSHI) in HIV-1 Disease Monitoring
Bruce Patterson
Part IV: Protocols for Model Organisms
27. Detection of Prokaryotic Cells with Fluorescence in situ Hybridization
Katrin Zwirglmaier
28. FISH as a Tool to Investigate Chromosome Behavior in Budding Yeast
Harry Scherthan and Josef Loidl
29. FISH on Chromosomes Derived from the Snail Model Organism Biomphalaria glabrata
Edwin C. Odoemelam, Nithya Raghavan, Wannaporn Ittiprasert, Andre Miller, Joanna M. Bridger, and Matty Knight
30. Fluorescence in situ Hybridization with Bacterial Artificial Chromosomes (BACs) to Mitotic
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