Cell culture techniques
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Bibliographic Information
Cell culture techniques
（Springer protocols, Neuromethods ; 56）
Humana Press, c2011
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Note
Includes bibliographical references and index
NM56--spine
Description and Table of Contents
Description
Societal, ethical, and cost-related issues, not to mention the need for sound scientific methods, have led to new and refined methods for the evaluation of health risks associated with neurotoxic compounds, relevant and predictive of exposure, relatively inexpensive, and ideally amenable to high throughput analysis and a reduction in animal use. Cell Culture Techniques presents thorough traditional chapters, such as those on various cell culture methods that have evolved over the years, as well as innovative approaches to neurotoxicologic testing. Accordingly, this detailed volume describes how stem cells, computational biology, and other novel powerful methods can now be applied to address the challenges of neurotoxic testing. As part of the Neuromethods series, this work provides the kind of intensive description and implementation advice that is crucial for getting optimal results in the laboratory.
Practical and authoritative, Cell Culture Techniques serves both the novice and the experienced neurotoxicologist by inspiring the further development of mechanistically-driven, cost-effective, high throughput series of tests needed to meet the many contemporary challenges.
Table of Contents
1. Guidance on Good Cell Culture Practice (GCCP)
Anna Bal-Price and Sandra Coecke
2. Induced Pluripotent Stem Cells (iPSCs): An Emerging Model System for the Study of Human Neurotoxicology
M. Diana Neely, Andrew M. Tidball, Asad A. Aboud, Kevin C. Ess, and Aaron B. Bowman
3. Neural Stem Cells
Roshan Tofighi, Christoffer Tamm, Michaela Moors, and Sandra Ceccatelli
4. Primary Cultures for Neurotoxicity Testing
Carme Sola, Rosa Cristofol, Cristina Sunol, and Coral Sanfeliu
5. Preparation and Use of Serum-Free Aggregating Brain Cell Cultures for Routine Neurotoxicity Screening
Paul Honegger and Marie-Gabrielle Zurich
6. Cell Culture to Investigate Neurotoxicity and Neurodegeneration Utilizing Caenorhabditis elegans
Michelle L. Tucci, Guy A. Caldwell, and Kim A. Caldwell
7. Modeling the Blood-Brain Barrier
Marie-Pierre Dehouck, Elodie Vandenhaute, Lucie Dehouck, Emmanuel Sevin, Anne-Marie Lenfant, Yannick Delplace, Dorothee Hallier-Vanuxeem, Maxime Culot, and Romeo Cecchelli
8. In vitro Models of the Blood-Cerebrospinal Fluid Barrier and Their Use in Neurotoxicological Research
Nathalie Strazielle and Jean-Francois Ghersi-Egea
9. Introducing Cloned Genes into Cultured Neurons Providing Novel In vitro Models for Neuropathology and Neurotoxicity Studies
Marcelo Farina, Jordi Berenguer, Sebastian Pons, Joao Batista Teixeira da Rocha, and Michael Aschner
10. P19 Embryonic Carcinoma Cell Line: A Model To Study Gene-Environment Interactions
Joseph Bressler, Cliona O'Driscoll, Cathleen Marshall, and Walter Kaufmann
11. Signal Transduction and Neurotoxicity: What Can We Learn from Experimental Culture Systems?
Lucio G. Costa, Gennaro Giordano, and Marina Guizzetti
12. Neurite Degeneration in Human Neuronal SH-SY5Y Cells as an Indicator of Axonopathy
Anna Forsby
13. The Use of Differentiating N2a and C6 Cell Lines for Studies of Organophosphate Toxicity
Alan J. Hargreaves, Magda Sachana, and John Flaskos
14. Assessing Toxic Injuries of Experimental Therapeutics to the Crystalline Lens Using Lens Explant Culture
Michael D. Aleo
15. Necrosis, Apoptosis, and Autophagy: Mechanisms of Neuronal and Glial Cell Death
Michael Fricker and Aviva M. Tolkovsky
16. Inflammation and Reactive Oxygen/Nitrogen Species in Glial/Neuronal Cultures
Jonas J. Neher, Guy C. Brown, Agnieszka Kinsner-Ovaskainen, and Anna Bal-Price
17. Neuronal Oxidative Injury and Biomarkers of Lipid Peroxidation
Dejan Milatovic and Michael Aschner
18. Analysis of Protein Targets by Oxidative Stress Using the Oxyblot and Biotin-Avidin-Capture Methodology
Jeannette N. Stankowski, Simona G. Codreanu, Daniel C. Liebler, and BethAnn McLaughlin
19. Catecholaminergic Cell Lines for the Study of Dopamine Metabolism and Neurotoxicity
Juan Segura-Aguilar
20. 13C NMR Spectroscopy and Mass Spectrometry Analysis of Intermediary Metabolism in Cultured Neural Cells
Ursula Sonnewald, Arne Schousboe, and Helle S. Waagepetersen
21. Culture Models for the Study of Amino Acid Transport and Metabolism
Marta Sidoryk-Wegrzynowicz and Michael Aschner
22. Neurotransmitter Transporters and Anticonvulsant Drug Development
Arne Schousboe, Karsten K. Madsen, and H. Steve White
23. Ion Channel Electrophysiology in Cultured Neurons
Toshio Narahashi
24. Neurotoxicity Assessment by Recording Electrical Activity from Neuronal Networks on Microelectrode Array Neurochips
Dieter G. Weiss
25. GABAA Receptor Binding and Ion Channel Function in Primary Neuronal Cultures for Neuropharmacology/Neurotoxicity Testing
Cristina Sunol and Daniel A. Garcia
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