Flow cytometry protocols
Author(s)
Bibliographic Information
Flow cytometry protocols
（Methods in molecular biology / John M. Walker, series editor, 699）（Springer protocols）
Humana Press, c2011
3rd ed
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Note
Includes bibliographical references and index
Description and Table of Contents
Description
With rapid improvements in instrumentation, lasers, fluorophores, and data analysis software, flow cytometry is riding the crest of unprecedented, innovative advances. This thoroughly revised and up-to-date third edition of Flow Cytometry Protocols highlights the expanding contribution of flow cytometry to basic biological research and diagnostic medicine. Written by leading experts in the field, the book presents cutting-edge topics such as polychromatic, quantitative, and high throughput flow cytometry, novel multiparametric data analysis which breaks the dimensionality barrier, standard practice and safety measures for aerosol-generating cell sorting, conventional and imaging flow cytometry as well as minimalist imaging cytometry. As a volume in the highly successful Methods in Molecular Biology (TM) series, chapters contain brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and extensive notes on troubleshooting and avoiding known pitfalls.
Authoritative and comprehensive, Flow Cytometry Protocols, Third Edition presents established as well as new flow cytometric methodologies in order to introduce beginning users to basic applications while opening new avenues of innovation for seasoned users.
Table of Contents
1. Flow Cytometry: An Introduction
Alice L. Givan
2. Breaking the Dimensionality Barrier
C. Bruce Bagwell
3. Quantitative Fluorescence Measurements with Multicolor Flow Cytometry
Lili Wang, Adolfas K. Gaigalas, and Ming Yan
4. Quantum Dots for Quantitative Flow Cytometry
Yang Wu, Larry A. Sklar, and Tione Buranda
5. Bead-Based Multiplexed Analysis of Analytes by Flow Cytometry
Henri C. van der Heyde and Irene Gramaglia
6. Flow-Based Combinatorial Antibody Profiling: An Integrated Approach to Cell Characterization
Shane Bruckner, Ling Wang, Ruiling Yuan, Perry Haaland, and Amitabh Gaur
7. Tracking Immune Cell Proliferation and Cytotoxic Potential Using Flow Cytometry
Joseph D. Tario, Jr., Katharine A. Muirhead, Dalin Pan, Mark E. Munson, and Paul K. Wallace
8. Multiparameter Intracellular Cytokine Staining
Patricia Lovelace and Holden T. Maecker
9. Phospho Flow Cytometry Methods for Analysis of Kinase Signaling in Cell Lines and Primary Human Blood Samples
Peter O. Krutzik, Angelica Trejo, Kenneth R. Schulz, and Garry P. Nolan
10. Multiparametric Analysis of Apoptosis by Flow Cytometry
William G. Telford, Akira Komoriya, Beverly Z. Packard, and C. Bruce Bagwell
11. Multiparameter Cell Cycle Analysis
James W. Jacobberger, R. Michael Sramkoski, and Tammy Stefan
12. Rare Event Detection and Analysis in Flow Cytometry: Bone Marrow Mesenchymal Stem Cells, Breast Cancer Stem/Progenitor Cells in Malignant Effusions, and Pericytes in Disaggregated Adipose Tissue
Ludovic Zimmerlin, Vera S. Donnenberg, and Albert D. Donnenberg
13. Flow Cytometric-Based Identification of Immature Myeloerythroid Development
Cornelis J.H. Pronk and David Bryder
14. Flow Cytometry Immunophenotyping of Hematolymphoid Neoplasia
Katherine R. Calvo, Catharine S. McCoy, and Maryalice Stetler-Stevenson
15. Flow Cytometry Assays in Primary Immunodeficiency Diseases
Maurice R.G. O'Gorman, Joshua Zollett, and Nicolas Bensen
16. Flow Cytometric Analysis of Microparticles
Henri C. van der Heyde, Irene Gramaglia, Valery Combes, Thaddeus C. George, and Georges E. Grau
17. Noncytotoxic DsRed Derivatives for Whole-Cell Labeling
Rita L. Strack, Robert J. Keenan, and Benjamin S. Glick
18. Flow Cytometric FRET Analysis of Protein Interaction
Gyoergy Vereb, Peter Nagy, and Janos Szoellosi
19. Fluorescent Protein-Assisted Purification for Gene Expression Profiling
M. Raza Zaidi, Chi-Ping Day, and Glenn Merlino
20. Multiparametric Analysis, Sorting, and Transcriptional Profiling of Plant Protoplasts and Nuclei According to Cell Type
David W. Galbraith, Jaroslav Janda, and Georgina M. Lambert
21. Lentiviral Fluorescent Protein Expression Vectors for Biotinylation Proteomics
Irene Riz, Teresa S. Hawley, and Robert G. Hawley
22. Standard Practice for Cell Sorting in a BSL-3 Facility
Stephen P. Perfetto, David R. Ambrozak, Richard Nguyen, Mario Roederer, Richard A. Koup, and Kevin L. Holmes
23. The Cytometric Future: It Ain't Necessarily Flow!
Howard M. Shapiro
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