DNA nanotechnology : methods and protocols
著者
書誌事項
DNA nanotechnology : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, 749）（Springer protocols）
Humana Press, c2011
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
Currently, nanotechnology is exposing the properties of DNA in unprecedented detail leading to new insights on the biological behavior and function of DNA. With the structural perfection of a self-assembling DNA nano-object, such as a DNA origami, it is clear how complex DNA is as a molecule, leading researchers to wonder how many different constructs could be designed and realized. DNA Nanotechnology: Methods and Protocols shows the procedures to follow in order to repeat methods that lead to such constructs or to the mastering of the characterization techniques used to study them. The chapters of this book are roughly divided into two parts: some cover the methods for preparing the nanostructures, from the rationale of the operations to the techniques for their handling, while other chapters deal more directly with advanced instrumental techniques that can manipulate and characterize molecules and nanostructures. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and accessible, DNA Nanotechnology: Methods and Protocols serves as an ideal guide to scientists of all backgrounds and aims to ignite interest and spur activity in this young and rapidly growing research field.
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
目次
1. Synthesis and Characterization of Self-assembled DNA Nanostructures
Chenxiang Lin, Yonggang Ke, Rahul Chhabra, Jaswinder Sharma, Yan Liu, and Hao Yan
2. Protocols for Self-assembly and Imaging of DNA Nanostructures
Thomas L. Sobey and Friedrich C. Simmel
3. Self-Assembly of Metal-DNA Triangles and DNA Nanotubes with Synthetic Junctions
Hua Yang, Pik Kwan Lo, Christopher K. McLaughlin, Graham D. Hamblin, Faisal A. Aldaye, and Hanadi F. Sleiman
4. DNA-Templated Pd Conductive Metallic Nanowires
Khoa Nguyen, Stephane Campidelli, and Arianna Filoramo
5. A Method to Map Spatiotemporal pH Changes Inside Living Cells using a pH Triggered DNA Nanoswitch
Souvik Modi and Yamuna Krishnan
6. Control of Helical Handedness in DNA and PNA Nanostructures
Roberto Corradini, Tullia Tedeschi, Stefano Sforza, Mark M. Green, and Rosangela Marchelli
7. G-quartet, G-quadruplex, and G-wire Regulated by Chemical Stimuli
Daisuke Miyoshi and Naoki Sugimoto
8. Preparation and Atomic Force Microscopy of Quadruplex DNA
James Vesenka
9. Synthesis of Long DNA-based Nanowires
Alexander Kotlyar
10. G-wire Synthesis and Modification with Gold-nanoparticle
Christian Leiterer, Andrea Csaki, and Wolfgang Fritzsche
11. Preparation of DNA Nanostructures with Repetitive Binding Motifs by Rolling Circle Amplification
Edda Reiss, Ralph Hoelzel, and Frank F. Bier
12. Controlled Confinement of DNA at the Nanoscale: Nanofabrication and Surface Bio-functionalization
Matteo Palma, Justin Abramson, Alon A. Gorodetsky, Colin Nuckolls, Michael P. Sheetz, Shalom J. Wind, and James Hone
13. Templated Assembly of DNA Origami Gold Nanoparticle Arrays on Lithographically Patterned Surfaces
Albert M. Hung and Jennifer N. Cha
14. DNA-modified Single Crystal and Nanoporous Silicon
Andrew Houlton, Bernard A. Connolly, Andrew R. Pike, and Benjamin R. Horrocks
15. The Atomic Force Microscopy as a Lithographic Tool: Nanografting of DNA Nanostructures for Biosensing Applications
Matteo Castronovo and Denis Scaini
16. Trapping and Immobilization of DNA Molecules Between Nanoelectrodes
Anton Kuzyk, J. Jussi Toppari, and Paivi Toerma
17. DNA Contour Length Measurements as a Tool for the Structural Analysis of DNA and Nucleoprotein Complexes
Claudio Rivetti
18. DNA Molecular Handles for Single Molecule Protein Folding Studies by Optical Tweezers
Ciro Cecconi, Elizabeth A. Shank, Susan Marqusee, and Carlos Bustamante
19. Optimal Practices for Surface-tethered Single Molecule Total Internal Reflection Fluorescence Resonance Energy Transfer Analysis
Matt V. Fagerburg and Sanford H. Leuba
20. Engineering Mononucleosomes for Single-Pair FRET Experiments
Wiepke J.A. Koopmans, Ruth Buning, and John van Noort
21. Measuring DNA-Protein Binding Affinity on a Single Molecule Using Optical Tweezers
Micah J. McCauley and Mark C. Williams
22. Modeling Nanopores for Sequencing DNA
Jeffrey R. Comer, David B. Wells, and Aleksei Aksimentiev
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