Cell migration : developmental methods and protocols
著者
書誌事項
Cell migration : developmental methods and protocols
（Methods in molecular biology / John M. Walker, series editor, 769）（Springer protocols）
Humana Press, c2011
2nd ed
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
Cell migration is a key component of many biological processes including embryonic development, immune responses, wound healing, organ regeneration, and cancer cell metastasis, thus making it an exciting and crucial field of study. The aim of Cell Migration: Developmental Methods and Protocols, Second Edition is to bring together a wide range of these techniques from the more basic migration assays, which are still the foundation of many cell migration studies, to state-of-the-art techniques and recent technical advances. Divided into three convenient parts, the volume begins with a number of basic in vitro migration assays including measurements of wound healing, cell scattering, invasion, and chemotaxis, as well as more complex measurements of transendothelial migration, the use of microfluidic chambers, and imaging cell migration in 3D. It continues with procedures for the imaging and measurement of cell migration in vivo including protocols for the use of chick, drosophila, and zebrafish embryos, and methods to measure metastatic spread and angiogenesis in mice, then concludes with a vital section on emerging techniques in the cell migration field including the use of TIRF, FRAP, and FRET microscopy. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes from the experts on troubleshooting and avoiding known pitfalls.
Comprehensive and up-to-date, Cell Migration: Developmental Methods and Protocols, Second Edition provides a comprehensive catalogue of techniques for the study of cell migration that can be used as a useful reference source for any researcher who wishes to explore this significant area of cell biology.
目次
1. Cell Migration: An Overview
Miguel Vicente-Manzanares and Alan Rick Horwitz
2. Scratch-Wound Assay
Giles Cory
3. HGF-Induced DU145 Cell Scatter Assay
Sally T. Fram, Claire M. Wells, and Gareth E. Jones
4. Using the Dunn Chemotaxis Chamber to Analyze Primary Cell Migration in Real Time
Sanjay Chaubey, Anne J. Ridley, and Claire M. Wells
5. Imaging Cells Within 3D Cell Derived Matrix
Samantha J. King and Maddy Parsons
6. Live Cell Imaging of Neuronal Growth Cone Motility and Guidance In Vitro
Daniel M. Suter
7. Boyden Chamber
Marco Falasca, Claudio Raimondi, and Tania Maffucci
8. Transwell (R) Invasion Assays
John Marshall
9. Imaging Podosome Dynamics and Matrix Degradation
Taylor W. Starnes, Christa L. Cortesio, and Anna Huttenlocher
10. Endothelial Cell Migration Under Flow
Beata Wojciak-Stothard
11. In Vitro Analysis of Chemotactic Leukocyte Migration in 3D Environments
Michael Sixt
12. Quantification of Transendothelial Migration Using Three-Dimensional Confocal Microscopy
Robert J. Cain, Barbara Borda d'Agua, and Anne J. Ridley
13. Chemotaxis of Slow Migrating Mammalian Cells Analyzed by Video Microscopy
Roman Zantl and Elias Horn
14. Live Cell Fluorescence Microscopy Techniques
Shawn A. Galdeen and Alison J. North
15. Measuring Invasion in an Organotypic Model
Veronika Jenei, Maria L. Nystrom, and Gareth J. Thomas
16. Analysis of Cell Migration Using Caenorhabditis elegans as a Model System
Ming-Ching Wong, Maria Martynovsky, and Jean E. Schwarzbauer
17. Drosophila Hemocyte Migration: An In Vivo Assay for Directional Cell Migration
Carolina G.A. Moreira, Jennifer C. Regan, Anna Zaidman-Remy, Antonio Jacinto, and Soren Prag
18. Measuring Inflammatory Cell Migration in the Zebrafish
Philip M. Elks, Catherine A. Loynes, and Stephen A. Renshaw
19. Border Cell Migration: A Model System for Live Imaging and Genetic Analysis of Collective Cell Movement
Mohit Prasad, Xiaobo Wang, Li He, and Denise J. Montell
20. Assessment of Development and Chemotaxis in Dictyostelium discoideum Mutants
Yulia Artemenko, Kristen F. Swaney, and Peter N. Devreotes
21. Simple Experimental and Spontaneous Metastasis Assays in Mice
Gary M. Box and Suzanne A. Eccles
22. Two-Photon Intravital Multicolour Imaging to Study Metastatic Behaviour
of Cancer Cells In Vivo
Sylvia E. Le Devedec, Wies van Roosmalen, Chantal Pont, Reshma Lalai, Hans de Bont, and Bob van de Water
23. Measuring Angiogenesis in Mice
Bernardo Tavora and Kairbaan Hodivala-Dilke
24. Time-Lapse Imaging of Chick Cardiac Precursor Cells
Junfang Song, Qiaoyun Yue, and Andrea Munsterberg
25. Characterizing System Performance in Total Internal Reflection Fluorescence Microscopy
Juliane P. Schwarz, Ireen Koenig, and Kurt I. Anderson
26. Fluorescence Recovery After Photobleaching
Alex Carisey, Matthew Stroud, Ricky Tsang, and Christoph Ballestrem
27. Measuring FRET Using Time-Resolved FLIM
Penny E. Morton and Maddy Parsons
28. Cell Migration in Confinement: A Micro-Channel Based Assay
Melina L. Heuze, Olivier Collin, Emmanuel Terriac, Ana-Maria Lennon-Dumenil, and Matthieu Piel
29. Functional Screening with a Live Cell Imaging Based Random Cell Migration Assay
Wies van Roosmalen, Sylvia E. Le Devedec, Sandra Zovko, Hans de Bont, and Bob van de Water
30. Beads on the Run: Beads as Alternative Tools for Chemotaxis Assays
Eric Theveneau and Roberto Mayor
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