Signal transduction protocols

著者

    • Luttrell, Louis M.
    • Ferguson, Stephen S. G.

書誌事項

Signal transduction protocols

edited by Louis M. Luttrell, Stephen S. G. Ferguson

（Methods in molecular biology / John M. Walker, series editor, v. 756）

Humana Press, c2011

3rd ed.

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注記

Includes bibliographical references and index

内容説明・目次

内容説明

It is increasingly clear that signal transduction is a highly organized and integrated process. Divided into two convenient sections, Signal Transduction Protocols, Third Edition focuses on experimental approaches to better understand the complexity of signal transduction. Introductory chapters provide perspective on several of the challenges involved in signal transduction research and offer guidance on selecting the best approaches to various types of questions. The individual chapters provide detailed experimental protocols covering various topics, from the effects of ligand binding on receptor conformation and effector coupling, to moving inside the cell in order to capture the spatial and temporal characteristics of signaling events. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Signal Transduction Protocols serves as an ideal guide to scientists of all backgrounds and is a valuable resource for future progress in the field of signal transduction research.

目次

Part I. Overviews 1. Refining Efficacy: Allosterism and Bias in G Protein-Coupled Receptor Signaling Louis M. Luttrell and Terry P. Kenakin 2. Imaging-based Approaches to Understanding G Protein-Coupled Receptor Signaling Complexes Darlaine Petrin and Terence E. Hebert 3. Improving Drug Discovery with Contextual Assays and Cellular Systems Analysis John K. Westwick and Jane E. Lamerdin 4. RGS-insensitive Ga Subunits: Probes of G Subtype-selective Signaling and Physiological Functions of RGS Proteins Kuljeet Kaur, Jason M. Kehrl, Raelene A. Charbeneau, and Richard R. Neubig 5. Bioinformatic Approaches to Metabolic Pathways Analysis Stuart Maudsley, Wayne Chadwick, Liyun Wang, Yu Zhou, Bronwen Martin, and Sung Park Part II. Receptor - Ligand Interactions 6. Studying Ligand Efficacy at G Protein-Coupled Receptors Using FRET Jean-Pierre Vilardaga 7. Using BRET to Detect Ligand-Specific Conformational Changes in Preformed Signaling Complexes Nicolas Audet and Graciela Pineyro Part III. Receptor - Receptor Interactions 8. Reconstitution of G Protein-Coupled Receptors into a Model Bilayer System: Reconstituted High Density Lipoprotein Particles (rHDL) Gisselle A. Velez-Ruiz and Roger K. Sunahara 9. Using Quantitative BRET to Assess G Protein-Coupled Receptor Homo- and Hetero-Dimerization Lamia Achour, Maud Kamal, Ralf Jockers, and Stefano Marullo 10. Cell-Surface Protein-Protein Interaction Analysis with Time-Resolved FRET and Snap-tag Technologies: Application to G Protein-Coupled Receptor Oligomerization Laetitia Comps-Agrar, Damien Maurel, Philippe Rondard, Jean-Philippe Pin, Trinquet Eric, and Laurent Prezeau 11. Analysis of GPCR/Ion Channel Interactions Christophe Altier and Gerald W. Zamponi Part IV. Receptor - Effector Coupling 12. Multicolor BiFC Analysis of G Protein Complex Formation and Localization Thomas R. Hynes, Evan A. Yost, Stacy M. Yost, and Catherine H. Berlot 13. Real-Time BRET Assays to Measure G Protein/Effector Interactions Darlaine Petrin, Melanie Robitaille, and Terence E. Hebert 14. Luminescent Biosensors for Real-Time Monitoring of Intracellular cAMP Brock F. Binkowski, Frank Fan, and Keith V. Wood 15. Simultaneous Real Time Imaging of Signal Oscillations Using Multiple Fluorescence-Based Reporters Lianne B. Dale and Stephen S. G. Ferguson Part V. Spatial Control of Signal Transduction 16. Using FRET-based Reporters to Visualize Subcellular Dynamics of Protein Kinase A Activity Charlene Depry and Jin Zhang 17. Genetically Encoded Fluorescent Reporters to Visualize Protein Kinase C Activation in Live Cells Lisa L. Gallegos and Alexandra C. Newton 18. Visualizing Receptor Endocytosis and Trafficking Ali Salahpour and Larry S. Barak 19. Investigating G Protein-Coupled Receptor Endocytosis and Trafficking by TIR-FM Guillermo A. Yudowski and Mark von Zastrow 20. Visualizing G Protein-Coupled Receptor Signalsomes Using Confocal Immunofluorescence Microscopy Sudha K. Shenoy Part VI. Protein - Protein Interactions 21. Detection and Characterization of Receptor Interactions with PDZ Domains Stefanie L. Ritter and Randy A. Hall 22. Tandem Affinity Purification and Identification of Heterotrimeric G-Protein Associated Proteins Syed M. Ahmed, Avais M. Daulat, and Stephane Angers 23. Study of G Protein-Coupled Receptor/ssarrestin Interactions within Endosomes Using FRAP Benjamin Aguila, May Simaan, and Stephane A. Laporte 24. Disrupting Protein Complexes Using Tat-tagged Peptide Mimics Shupeng Li, Sheng Chen, Yu Tian Wang, and Fang Liu 25. Protein-fragment Complementation Assays for Large-Scale Analysis, Functional Dissection and Dynamic Studies of Protein-Protein Interactions in Living Cells Stephen W. Michnick, Po Hien Ear, Christian Landry, Mohan K. Malleshaiah, and Vincent Messier

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