Nitrogen fixation : methods and protocols

著者

• Ribbe, Markus W.

書誌事項

Nitrogen fixation : methods and protocols

edited by Markus W. Ribbe

（Methods in molecular biology / John M. Walker, series editor, 766）（Springer protocols）

Humana Press, c2011

注記

Includes bibliographical references and index

内容説明・目次

内容説明

Few problems in protein biochemistry have proven to be as challenging and recalcitrant as the molecular description of nitrogenase, the catalyst of one of the most remarkable chemical transformations in biological systems: the nucleotide-dependent reduction of atmospheric dinitrogen to bioavailable ammonia. In Nitrogen Fixation: Methods and Protocols, recognized experts in the field provide an up-to-date, in-depth overview of the methods that have been applied to studying the nitrogenase at a molecular level, ranging from genetic, biochemical, spectroscopic, and chemical methods to theoretical calculations. In addition, techniques used to study an enzyme system that is homologous to nitrogenase are described in this book. Written in the highly successful Methods in Molecular Biology (TM) series format, methods chapters include introductions to their respective chapters, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and cutting-edge, Nitrogen Fixation: Methods and Protocols will be useful for anyone interested in nitrogenase research and willing to venture further toward addressing the remaining mechanistic and biosynthetic questions of this fascinating enzyme system.

目次

Part I: Nitrogen Fixation 1. Historic Overview of Nitrogenase Research Yilin Hu and Markus W. Ribbe 2. Mechanism of Mo-Dependent Nitrogenase Zhi-Yong Yang, Karamatullah Danyal, and Lance C. Seefeldt 3. Assembly of Nitrogenase MoFe Protein Chi-Chung Lee, Aaron W. Fay, Jared A. Wiig, Markus W. Ribbe, and Yilin Hu 4. Genomic Analysis of Nitrogen Fixation Ina P. O'Carroll and Patricia C. Dos Santos 5. Enzymatic Systems with Homology to Nitrogenase Markus J. Broecker and Jurgen Moser Part II: Genetic and Biochemical Methods 6. Molecular Biology and Genetic Engineering in Nitrogen Fixation Patricia Dos Santos 7. Purification of Nitrogenase Proteins Jared A. Wiig, Chi-Chung Lee, Aaron W. Fay, Yilin Hu, and Markus W. Ribbe 8. Assays of Nitrogenase Reaction Products William E. Newton and Michael J. Dilworth 9. Methods for Nitrogenase-Like Dark Operative Protochlorophyllide Oxidoreductase Markus J. Broecker and Jurgen Moser Part III: Spectroscopic Methods 10. X-Ray Crystallography Lauren E. Roth and F. Akif Tezcan 11. X-Ray Absorption Spectroscopy Serena DeBeer 12. Small Angle X-Ray Scattering Spectroscopy David W. Mulder and John W. Peters 13. Electron Paramagnetic Resonance Spectroscopy Karamatullah Danyal, Zhi-Yong Yang, and Lance C. Seefeldt 14. Magnetic Circular Dichroism Spectroscopy Brian J. Hales 15. Moessbauer Spectroscopy Boi Hanh Huynh Part IV: Chemical Methods 16. Protocols for Cofactor Isoation of Nitrogenase Aaron W. Fay, Chi-Chung Lee, Jared A. Wiig, Yilin Hu, and Markus W. Ribbe 17. Techniques for Functional and Structural Modeling of Nitrogenase Patrick L. Holland Part V: Theoretical Calculations 18. Nitrogenase Structure and Function Relationships by Density Functional Theory Travis V. Harris and Robert K. Szilagyi 19. Modeling the MoFe Nitrogenase System with Broken Symmetry Density Functional Theory Gregory M. Sandala and Louis Noodleman

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詳細情報

• NII書誌ID(NCID): BB06890364
• ISBN:
  • 9781617791932
• LCCN: 2011931959
• 出版国コード: us
• タイトル言語コード: eng
• 本文言語コード: eng
• 出版地: New York, N.Y.
• ページ数/冊数: x, 326 p.
• 大きさ: 27 cm
• 件名:
  • LCSH : Nitrogen -- Fixation
• 親書誌ID:
  • BA10794673
  • BA88873632