Plant kinases : methods and protocols
著者
書誌事項
Plant kinases : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, 779）（Springer protocols）
Humana Press, c2011
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
Modification of target protein properties by reversible phosphorylation events has been found to be one of the most prominent cellular control processes in all organisms. Recent advances in the areas of molecular biology and biochemistry are presenting new possibilities for reaching an unprecedented depth and a proteome-wide understanding of phosphorylation processes in plants as well as in other species. The major goal of Plant Kinases: Methods and Protocols is to provide the experimentalist with a detailed account of the practical steps necessary for successfully carrying out each protocol in his or her own laboratory. Plant protein kinases specifically addressed in this volume are members of the plant MAP kinase cascade, cyclin- and Calcium-dependent protein kinases, and plant sensor and receptor kinases. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and accessible, Plant Kinases: Methods and Protocols will prove a useful laboratory companion to both novice and seasoned researchers by facilitating the practical work that will lead them to new and exciting insights in this dynamic field.
目次
1. Guide to the Book Plant Kinases
Nico Dissmeyer and Arp Schnittger
2. The Age of Protein Kinases
Nico Dissmeyer and Arp Schnittger
3. Expression and Purification of Active Protein Kinases from Wheat Germ Extracts
Boglarka Sonkoly, Viola Bardoczy, and Tamas Meszaros
4. Measurement of Plant Cyclin-Dependent Kinase Activity Using Immunoprecipitation-Coupled and Affinity Purification-Based Kinase Assays and the Baculovirus Expression System
Hirofumi Harashima and Masami Sekine
5. Mitogen-Activated Protein Kinase Activity and Reporter Gene Assays in Plants
Robert Doczi, Elizabeth Hatzimasoura, and Laszlo Boegre
6. Use of Phospho-site Substitutions to Analyze the Biological Relevance of Phosphorylation Events in Regulatory Networks
Nico Dissmeyer and Arp Schnittger
7. Bacterial Assay to Study Plant Sensor Histidine Kinases
Lukas Spichal
8. Substrate Analysis of Arabidopsis PP2C-type Protein Phosphatase
Julija Umbrasaite, Alois Schweighofer and Irute Meskiene
9. Modulating and Monitoring MAPK Activity During Programmed Cell Death in Pollen
Shutian Li and Vernonica E. Franklin-Tong
10. Sensitizing Plant Protein Kinases to Specific Inhibition by ATP-Competitive Molecules
Dor Salomon, Chao Zhang, Kevan M. Shokat, and Guido Sessa
11. Fluorescence Fluctuation Analysis of Receptor Kinase Dimerization
Mark A. Hink, Sacco C. de Vries, and Antonie J.W.G. Visser
12. Quantifying Degradation Rates of Trans-Membrane Receptor Kinases
Niko Geldner
13. Fluorescence Correlation Spectroscopy and Fluorescence Recovery after Photobleaching to Study Receptor Kinase Mobility in planta
Mark A.C.J. Kwaaitaal, Marieke Schor, Mark A. Hink, Antonie J.W.G. Visser, and Sacco C. de Vries
14. Bimolecular-Fluorescence Complementation Assay to Monitor Kinase-Substrate Interactions in vivo
Stefan Pusch, Nico Dissmeyer, and Arp Schnittger
15. Chemical Genetic Analysis of Protein Kinase Function in Plants
Mike Boehmer, Michael Boelker, and Tina Romeis
16. Modified Metal Oxide Affinity Enrichment Combined with 2D-PAGE and Analysis of Phosphoproteomes
Thomas Colby, Horst Roehrig, Anne Harzen, and Jurgen Schmidt
17. Phosphoproteomics Using iTRAQ
Alexandra M.E. Jones and Thomas S. Nuhse
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