Methods and resources to understand cystic fibrosis

Author(s)

• Amaral, Margarida D.
• Kunzelmann, Karl

Bibliographic Information

Methods and resources to understand cystic fibrosis

edited by Margarida D. Amaral, Karl Kunzelmann

（Methods in molecular biology / John M. Walker, series editor, v. 742 . Cystic fibrosis : diagnosis and protocols ; v. 2）（Springer protocols）

Humana Press, c2011

Note

Includes bibliographical references and indexes

Description and Table of Contents

Description

Despite the many milestones in cystic fibrosis (CF) research, progress toward curing the disease has been slow, and it is increasingly difficult to grasp and use the already wide and still growing range of diverse methods currently employed to study CF so as to understand it in its multidisciplinary nature. Cystic Fibrosis: Diagnosis and Protocols aims to provide the CF research community and related researchers with a very wide range of high-quality experimental tools, as an easy way to grasp and use classical and novel methods applied to cystic fibrosis. Volume II: Methods and Resources to Understand Cystic Fibrosis focuses on pathophysiology, Omics approaches, and a variety of key resources recently made available for CF research. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and practical, Cystic Fibrosis: Diagnosis and Protocols will provide readers with optimal working tools to address pressing questions in the best technical way, while helping all of us, as a research and clinical community, to move faster hand-in-hand toward unravelling the secrets of this challenging disorder and cure it.

Table of Contents

Part I: Pathophysiology of Cystic Fibrosis 1. Introduction to Part I: Overview of Approaches to Study Cystic Fibrosis Pathophysiology Mark T. Clunes and Richard C. Boucher 2. Imaging CFTR Protein Localization in Cultured Cells and Tissues Silvia M. Kreda and Martina Gentzsch 3. CFTR Regulation of Epithelial Sodium Channel Yawar J. Qadri, Estelle Cormet-Boyaka, Dale J. Benos, and Bakhrom K. Berdiev 4. Methods for Evaluating Inflammation in Cystic Fibrosis Assem G. Ziady and Pamela B. Davis 5. Methods for ASL Measurements and Mucus Transport Rates in Cell Cultures Erin N. Worthington and Robert Tarran 6. Measurement of Fluid Secretion from Intact Airway Submucosal Glands Jeffrey J. Wine, Nam Soo Joo, Jae Young Choi, Hyungju Cho, Mauri E. Krouse, Jin V. Wu, Monal Khansaheb, Toshiya Irokawa, Juan Ianowski, John W. Hanrahan, Alan W. Cuthbert, and Kim V. Tran 7. Measurements of Intracellular Calcium Signals in Polarized Primary Cultures of Normal and Cystic Fibrosis Human Airway Epithelia Carla M.P. Ribeiro 8. Identification and Quantification of Mucin Expression Kristina A. Thomsson and Gunnar C. Hansson 9. Methods to Classify Bacterial Pathogens in Cystic Fibrosis Thomas Bjarnsholt, Xiaohui Chen Nielsen, Ulla Johansen, Lena Norgaard, and Niels Hoiby 10. Approaches to Study Differentiation and Repair of Human Airway Epithelial Cells Sophie Crespin, Marc Bacchetta, Song Huang, Tecla Dudez, Ludovic Wiszniewski, and Marc Chanson Part II: Omic Approaches to Study Cystic Fibrosis 11. Introduction to Part II: Omics in the Biology of Cystic Fibrosis William E. Balch 12. Microarray mRNA Expression Profiling to Study Cystic Fibrosis Shyam Ramachandran, Luka A. Clarke, Todd E. Scheetz, Margarida D. Amaral, and Paul B. McCray, Jr. 13. Quantitative Differential Proteomics of Cystic Fibrosis Cell Models by SILAC (Stable Isotope Labelling in Cell Culture) Ida Chiara Guerrera, Mario Ollero, Diane-Lore Vieu, and Aleksander Edelman 14. Application of Mass Spectrometry to Study Proteomics and Interactomics in Cystic Fibrosis William E. Balch and John R. Yates III 15. Functional Genomics Assays to Study CFTR Traffic and ENaC Function Joana Almaca, Shehrazade Dahimene, Nicole Appel, Christian Conrad, Karl Kunzelmann, Rainer Pepperkok, and Margarida D. Amaral 16. New Lipidomic Approaches in Cystic Fibrosis Mario Ollero, Ida Chiara Guerrera, Giuseppe Astarita, Daniele Piomelli, and Aleksander Edelman Part III: Resources 17. Introduction to Part III: Resources for CFTR Research Margarida D. Amaral 18. Primary Epithelial Cell Models for Cystic Fibrosis Research Scott H. Randell, M. Leslie Fulcher, Wanda O'Neal, and John C. Olsen 19. Comparative Biology of Cystic Fibrosis Animal Models John T. Fisher, Yulong Zhang, and John F. Engelhardt 20. CFTR Folding Consortium: Methods Available for Studies of CFTR Folding and Correction Kathryn W. Peters, Tsukasa Okiyoneda, William E. Balch, Ineke Braakman, Jeffrey L. Brodsky, William B. Guggino, Christopher M. Penland, Harvey B. Pollard, Eric J. Sorscher, William R. Skach, Philip J. Thomas, Gergely L. Lukacs, and Raymond A. Frizzell 21. Evaluation of the Disease Liability of CFTR Variants Patrick R. Sosnay, Carlo Castellani, Mary Corey, Ruslan Dorfman, Julian Zielenski, Rachel Karchin, Christopher M. Penland, and Garry R. Cutting

by "Nielsen BookData"

Details

• NCID: BB07170983
• ISBN:
  • 9781617791192
• Country Code: us
• Title Language Code: eng
• Text Language Code: eng
• Place of Publication: New York
• Pages/Volumes: xiv, 384 p.
• Size: 27 cm
• Classification:
  • LCC : RC858.C95
  • NLM : WI 820 C9969 2011
  • DC23 : 616.372
• Subject Headings:
  • LCSH : Cystic fibrosis
  • LCSH : Cystic fibrosis -- Diagnosis
  • MESH : Cystic Fibrosis
  • MESH : Cystic Fibrosis -- diagnosis
• Parent Bibliography ID:
  • BA10794673
  • BA88873632