Introduction to experimental biophysics : biological methods for physical scientists

著者

• Nadeau, Jay L.

書誌事項

Introduction to experimental biophysics : biological methods for physical scientists

Jay Nadeau

CRC Press/Taylor & Francis, c2012-

• : pbk
• laboratory guide

タイトル別名

Introduction to experimental biophysics : a laboratory guide

注記

[Main]: xxi, 641 p., [8] p. of plates (26 cm.); Laboratory guide: xxvii, 169 p. (21 cm.)

"This book is intended as a laboratory companion to An introduction to experimental biophysics: biological methods for physical scientists (Boca Raton, FL.: Taylor & Francis 2012)."--Preface of laboratory guide

Includes bibliographical references and index

内容説明・目次

巻冊次

: pbk ISBN 9781439829530

内容説明

Increasing numbers of physicists, chemists, and mathematicians are moving into biology, reading literature across disciplines, and mastering novel biochemical concepts. To succeed in this transition, researchers must understand on a practical level what is experimentally feasible. The number of experimental techniques in biology is vast and often specific to particular subject areas; nonetheless, there are a few basic methods that provide a conceptual underpinning for broad application. Introduction to Experimental Biophysics is the ideal benchtop companion for physical scientists interested in getting their hands wet. Assuming familiarity with basic physics and the scientific method but no previous background in biology or chemistry, this book provides: A thorough description of modern experimental and analytical techniques used in biological and biophysical research Practical information and step-by-step guidance on instrumentation and experimental design Recipes for common solutions and media, lists of important reagents, and a glossary of biological terms used Developed for graduate students in biomedical engineering, physics, chemical engineering, chemistry, mathematics, and computer science, Introduction to Experimental Biophysics is an essential resource for scientists to overcoming conceptual and technical barriers to working in a biology wet lab.

目次

Introduction and Background Basic Biochemistry Energies and Potentials Principles of Spectroscopy Cells DNA, RNA, Replication, and Transcription Translation and the Genetic Code Protein Folding and Trafficking Alternative Genetics What Is Cloning? Design of a Molecular Biology Experiment and How to Use This Book Questions and Problems Background Reading Molecular Cloning of DNA and RNA Introduction Obtaining and Storing Plasmids Selection of an Appropriate E. coli Amplification Strain: Transformation of E. coli with Plasmid Plasmid Amplification and Purification Plasmid Restriction Mapping and Agarose Gel Electrophoresis An Example of Cloning Experiment Cloning by the Polymerase Chain Reaction Sequencing RNA Methods Southern and Northern Blots Solutions for Large Cloning Problems and Multiple Inserts Mutagenesis and Directed Evolution Microarrays Summary Questions and Problems Background Reading Expression of Genes in Bacteria, Yeast, and Cultured Mammalian Cells Introduction Expressing Genes in Microorganisms Mammalian Cell Culture Transfection of Mammalian Cells I: Standard Techniques Transfection of Mammalian Cells II: Specialized Physical Methods for Special Occasions Transfection of Mammalian Cells III: Viruses Summary Questions and Problems Background Reading Protein Expression Methods Introduction Expression Systems Identification of a DNA Source Selecting an Expression Vector Subcloning into an Expression Vector Selection of an Expression Strain or Cell Line Protein Expression Checking Protein Expression (and Purity) Using SDS-PAGE Protein Isolation and Purification Chromatography Buffer Exchange and Concentration Example Experiment: Expression and Purification of Fluorescent Protein Dronpa Conclusions and Final Remarks Background Reading Protein Crystallization Introduction Crystallization of Macromolecules Preparation of Proteins for Crystallization Components of Crystallization Solutions Other Factors Affecting Crystallization Crystallization Strategies Example Experiment: Lysozyme Data Collection and Structure Determination Using X-Ray Crystallography A Special Case: Membrane Proteins Troubleshooting Q&A Conclusions and Final Remarks Questions and Problems Background Reading Introduction to Biological Light Microscopy Introduction The Physics of Microscopy: Magnification and Resolution Anatomy of a Biological Microscope Brightfield Imaging Techniques Basic Fluorescence Microscopy Fluorophores for Cell Labeling Fluorescent Proteins Multispectral Imaging Using Acousto-Optical Tunable Filters Advanced Techniques Summary and Remarks Questions and Problems Background Reading Quantitative Cell Culture Techniques Introduction Quantifying Bacterial Growth and Death Quantifying Mammalian Cells Flow Cytometry Example Experiment: Determining Leukemic B-Cells and T-Cells by Flow Cytometry Quantifying Viruses Measuring Cell Populations Using Quantitative PCR Summary and Final Remarks Questions and Problems Background Reading Semiconductor Nanoparticles (Quantum Dots) Introduction Quantum Dot Properties and Synthesis QD Applications Example Experiment: Conjugation of Quantum Dots to Dopamine and Quantifying the Effects on Fluorescence per Molecule Bound Summary and Remarks Questions and Problems Background Reading Gold Nanoparticles Introduction The Physics of Scattering and Spherical Metal Nanoparticles Synthesis of Gold Nanoparticles Characterization and Surface Modification of Gold Nanoparticles Applications for Colorimetric Detection and Microscopy Sample Experiment: Labeling Cells with Lectin-Tagged Au Nanoparticles Applications in Surface-Enhanced Raman Scattering Gold Nanoparticles as Photothermal Transducers Conclusion Questions and Problems Background Reading Surface Functionalization Techniques Introduction Preparing Monolayers Using Functional Silanes or Thiols Techniques for Characterizing Surface Monolayers Functionalization of Modified Surfaces Using Cross-Linkers Example Experiment: Preparing a Silane-Biotin-Streptavidin Sandwich on SiO2 Features on an Si Chip Preventing Nonspecific Binding of Biomolecules Assembling Membrane Proteins on Surfaces Testing the Function of Immobilized Proteins Conclusion and Final Remarks Questions and Problems Background Reading Electrophysiology Introduction Physical Basis and Circuit Models Solutions and Blockers Instrumentation Lipid Bilayer Setup Cell Patch-Clamp Setup: What Is Needed? The Art and Magic of Pipette Pulling Step-by-Step Guide to Perform a Whole-Cell Recording Example Experiment: Whole-Cell Recording on Cells A Brief Introduction to Single-Channel Modeling and Data Analysis Network Recording Conclusions and Final Remarks Questions and Problems Background Reading Spectroscopy Tools and Techniques Introduction Guiding Principles UV-Vis Absorbance Spectroscopy Fluorescence Spectroscopy Time-Resolved Emission Time-Resolved Absorption Infrared Spectroscopy Nuclear Magnetic Resonance Electron Paramagnetic Resonance Spectroscopy X-Ray Spectroscopy Example Experiment: Characterization of CdSe/ZnS Nanoparticle Bioconjugate Using UV-Vis, Fluorescence Emission, Time-Resolved Emission, FTIR, and EPR Spectroscopy Final Comments Questions and Problems Background Reading Appendix Glossary Index

巻冊次

laboratory guide ISBN 9781466557659

内容説明

Easily Get Started with Biological Experiments Introduction to Experimental Biophysics - A Laboratory Guide presents wet lab methods for courses in biophysics or molecular biology. A companion to the author's highly praised An Introduction to Experimental Biophysics: Biological Methods for Physical Scientists, this manual offers a flexible course plan that permits completion of the labs in either a full term or intensive summer course. Tested in a pedagogical setting, the experiments follow a logical progression beginning with a DNA construct. The book starts with the basics of molecular cloning: amplifying and purifying plasmid, plasmid mapping, and using restriction enzymes. Later experiments deal with more advanced, emerging techniques, such as the synthesis and characterization of quantum dots and gold nanoparticles, protein crystallization, and spectroscopic techniques. This accessible guide will help both students and instructors in molecular biology, biophysics, and biomedical engineering. Students will understand how to use a variety of techniques in biological experiments while instructors will get practical guidance on preparing the experiments.

目次

Molecular Cloning. Gene Expression. Fluorescent Reporter Genes in Mammalian Cells. Nanoparticles. Electrophysiology. Appendices. Index.

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詳細情報

• NII書誌ID(NCID): BB07488224
• ISBN:
  • 9781439829530
  • 9781466557659
• 出版国コード: us
• タイトル言語コード: eng
• 本文言語コード: eng
• 出版地: Boca Raton, Fla.
• ページ数/冊数: v.
• 大きさ: 21-26 cm
• 分類:
  • DC22 : 571.40724
  • NDLC : RA71
• 件名:
  • LCSH : Biophysics -- Experiments