MAP kinase signaling protocols
著者
書誌事項
MAP kinase signaling protocols
(Methods in molecular biology / John M. Walker, series editor, v. 661)
Humana Press, c2010
2nd ed
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
Mitogen-activated protein (MAP) kinase (MAPK) cascades are key signaling components that govern essentially all cellular processes evoked by any type of stimulation, and it has been well established that the malfunctioning of these cascades leads to various diseases including cancer, autoimmunity, and diabetes. In MAP Kinase Signaling Protocols, Second Edition, expert researchers fully update the popular first edition the key techniques used in the study of MAPK signaling cascades in various cellular contexts. This thorough volume explores essential topics such as activation and function of components of the MAPK signaling cascades, the study of MAPK cascades as transmitters of membranal receptor signals, structure-function relationships of MAPKs, studies on the regulation of MAPK cascades, the use of lower organisms, animal models, and human genetics in the study of MAPKs, as well as the study of MAPKs in specific systems and diseases. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters includes introductions to their respective subjects, lists of the necessary materials, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and cutting-edge, MAP Kinase Signaling Protocols, Second Edition aims to facilitate the study of MAPKs and allow for quicker progress in our knowledge of many vital cellular processes as well as devastating diseases.
目次
Part I: Activation and Function of Components of the MAP Kinase Signaling Cascades
1. The MAP Kinase Signaling Cascades: A System of Hundreds of Components Regulates a Diverse Array of Physiological Functions
Yonat Keshet and Rony Seger
2. Determination of ERK Activity: Anti-Phospho-ERK Antibodies and In vitro Phosphorylation
Shiri Procaccia, Sarah Kraus, and Rony Seger
3. Activation of SAPK/JNKs In vitro
Deborah N. Chadee and John M. Kyriakis
4. Activation of p38 and Determination of Its Activity
Huamin Zhou, Jianming Chen, and Jiahuai Han
5. Activity Assays for Extracellular Signal-Regulated Kinase 5
Kazuhiro Nakamura and Gary L. Johnson
6. Use of Inhibitors in the Study of MAP Kinases
Kimberly Burkhard and Paul Shapiro
Part II: Study of MAP Kinase Cascades as Transmitters of Membranal Receptor Signals
7. MAP Kinase Activation by Receptor Tyrosine Kinases: In Control of Cell Migration
Gabi Tarcic and Yosef Yarden
8. Activation of Ras and Rho GTPases and MAP Kinases by G Protein-Coupled Receptors
Mario Chiariello, Jose P. Vaque, Piero Crespo, and J. Silvio Gutkind
9. Regulation of MAP Kinase Signaling by Calcium
Colin D. White and David B. Sacks
10. Identification of Novel Substrates of MAP Kinase Cascades Using Bioengineered Kinases that Uniquely Utilize Analogs of ATP to Phosphorylate Substrates
Hui Zheng, Adnan Al-Ayoubi, and Scott T. Eblen
11. ERK-MAP Kinase Signaling in the Cytoplasm
Michelle C. Mendoza, Ekrem Emrah Er, and John Blenis
12. Lentiviral Vectors to Study the Differential Function of ERK1 and ERK2 MAP Kinases
Marzia Indrigo, Alessandro Papale, Daniel Orellana and Riccardo Brambilla
Part III: Structure-Function Relationships and Localization of MAP Kinases
13. Structural Studies of MAP Kinase Cascade Components
Elizabeth J. Goldsmith, Xiaoshan Min, Haixia He, and Tianjun Zhou
14. Analysis of MAP Kinases by Hydrogen Exchange Mass Spectrometry
Kevin M. Sours and Natalie G. Ahn
15. A 'Molecular Evolution' Approachfor Isolation of Intrinsically Active (MEK-Independent) MAP Kinases
Vered Levin-Salomon, Oded Livnah, and David Engelberg
16. Reconstitution of the Nuclear Transport of the MAP Kinase ERK2
Arif Jivan, Aarati Ranganathan, and Melanie H. Cobb
17. Localization and Trafficking of Fluorescently Tagged ERK1 and ERK2
Matilde Marchi, Riccardo Parra, Mario Costa, and Gian Michele Ratto
Part IV: Studies on the Regulation of MAP Kinase Cascades
18. Studying the Regulation of MAP Kinase by MAP Kinase Phosphatases in vitro and in Cell Systems
Celine Tarrega, Caroline Nunes-Xavier, Rocio Cejudo-Marin, Jorge Martin-Perez, and Rafael Pulido
19. Proteomic Analysis of Scaffold Proteins in the ERK Cascade
Melissa M. McKay and Deborah K. Morrison
20. Analysis of ERKs' Dimerization by Electrophoresis
Adan Pinto and Piero Crespo
21. MAP Kinase - SUMO Pathway Interactions
Shen-Hsi Yang and Andrew D. Sharrocks
22. Computational Modelling of Kinase Signalling Cascades
David Gilbert, Monika Heiner, and Rainer Breitling
Part V: Use of Lower Organisms, Animal Models, and Human Genetics in the Study of MAP Kinases
23. Analysis of MAP Kinase Activity in Yeast
Elaine A. Elion and Rupam Sahoo
24. Detection of RTK Pathway Activation in Drosophila Using Anti-dpERK Immunofluorescence Staining
Aharon Helman and Ze'ev Paroush
25. Studying MAP Kinase Pathways During Early Development of Xenopus laevis
Aviad Keren and Eyal Bengal
26. Deciphering Signaling Pathways In vivo: The Ras/Raf/Mek/Erk Cascade
Gergana Galabova-Kovacs and Manuela Baccarini
27. Mutational and Functional Analysis in Human Ras/MAP Kinase Genetic Syndromes
William E. Tidyman and Katherine A. Rauen
Part VI: Study of MAP Kinases in Specific Physiological Systems
28. Implication of the ERK Pathway on the Post Transcriptional Regulation of VEGF mRNA Stability
Khadija Essafi-Benkhadir, Jacques Pouyssegur, and Gilles Pages
29. Studies on MAP Kinase Signaling in the Immune System
Hongbo Chi and
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