Single molecule analysis : methods and protocols

著者

    • Peterman, Erwin J. G.
    • Wuite, Gijs J. L.

書誌事項

Single molecule analysis : methods and protocols

edited by Erwin J. G. Peterman and Gijs J. L. Wuite

（Methods in molecular biology / John M. Walker, series editor, 783）（Springer protocols）

Humana Press, c2011

  • : hbk

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注記

Includes bibliographical references and index

内容説明・目次

内容説明

Life scientists believe that life is driven, directed, and shaped by biomolecules working on their own or in concert. It is only in the last few decades that technological breakthroughs in sensitive fluorescence microscopy and single-molecule manipulation techniques have made it possible to observe and manipulate single biomolecules and measure their individual properties. The methodologies presented in Single Molecule Techniques: Methods and Protocols are being applied more and more to the study of biologically relevant molecules, such as DNA, DNA-binding proteins, and motor proteins, and are becoming commonplace in molecular biophysics, biochemistry, and molecular and cell biology. The aim of Single Molecule Techniques: Methods and Protocols is to provide a broad overview of single-molecule approaches applied to biomolecules on the basis of clear and concise protocols, including a solid introduction to the most widely used single-molecule techniques, such as optical tweezers, single-molecule fluorescence tools, atomic force microscopy, magnetic tweezers, and tethered particle motion. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Single Molecule Techniques: Methods and Protocols serves as an ideal guide to scientists of all backgrounds and provides a broad and thorough overview of the exciting and still-emerging field of single-molecule biology.

目次

1. Introduction to Optical Tweezers: Background, System Designs, and Commercial Solutions Joost van Mameren, Gijs J. L. Wuite, and Iddo Heller 2. Optical Trapping and Unfolding of RNA Katherine H. White and Koen Visscher 3. DNA Unzipping and Force Measurements with a Dual Optical Trap Ismail Cisse, Pierre Mangeol, and Ulrich Bockelmann 4. Probing the Force Generation and Stepping Behavior of Cytoplasmic Dynein Arne Gennerich and Samara L. Reck-Peterson 5. A Brief Introduction to Single-molecule Fluorescence Methods Siet M.J.L. van den Wildenberg, Bram Prevo, and Erwin J.G. Peterman 6. Fluorescent Labeling of Proteins Mauro Modesti 7. Fluorescence Imaging of Single Kinesin Motors on Immobilized Microtubules Till Korten, Bert Nitzsche, Chris Gell, Felix Ruhnow, Cecile Leduc, and Stefan Diez 8. Exploring Protein Superstructures and Dynamics in Live Bacterial Cells using Single-molecule and Superresolution Imaging Julie S. Biteen, Lucy Shapiro, and W. E. Moerner 9. Fluorescence Microscopy of Nanochannel-confined DNA Fredrik Persson, Fredrik Westerlund, and Jonas O. Tegenfeldt 10. Fluorescence Correlation Spectroscopy Patrick Ferrand, Jerome Wenger, and Herve Rigneault 11. Introduction to Atomic Force Microscopy Pedro J. de Pablo 12. Sample Preparation for SFM Imaging of DNA, Proteins, and DNA-protein Complexes Dejan Ristic, Humberto Sanchez, and Claire Wyman 13. Single Molecule Protein Unfolding and Refolding using Atomic Force Microscopy Thomas Bornschloegl and Matthias Rief 14. How to Perform a Nanoindentation Experiment on a Virus Wouter H. Roos 15. Magnetic Tweezers for Single-molecule Manipulation Yeonee Seol and Keir C. Neuman 16. Probing DNA topology using Tethered Particle Motion David Dunlap, Chiara Zurla, Carlo Manzo, and Laura Finzi

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