Integrin and cell adhesion molecules : methods and protocols

著者

• 島岡, 要 シマオカ, モトム

書誌事項

Integrin and cell adhesion molecules : methods and protocols

edited by Motomu Shimaoka

（Methods in molecular biology / John M. Walker, series editor, 757）（Springer protocols）

Humana Press, c2011

• : hbk

注記

Includes bibliographical references and index

内容説明・目次

内容説明

Integrins play pivotal roles not only across a wide range of physiological processes including tissue morphogenesis, immune responses, wound healing, and regulation of cell growth and differentiation, but also in numerous pathological phenomena such as autoimmunity, thrombosis, and cancer metastasis/progression. Therefore, investigations on integrins often demand multi-disciplinary approaches, making researchers long for a handy collection of comprehensive and practical protocols that detail experimental methods for studying integrin and related cell adhesion molecule functionality. Integrin and Cell Adhesion Molecules: Methods and Protocols aims to provide readers not only with basic protocols in studying integrin functions, but also with summaries on those state-of-the-art technologies that have been utilized for understanding integrin functionality at the cellular, molecular, structural, and organismal levels. Divided into six convenient sections, this detailed volume covers basic protocols for the study of integrin and related cell adhesion molecule functionality in vitro, illustrates structural biology approaches for studying integrins and related cell adhesion molecules, focuses on emerging imaging technologies for investigating cell migration, presents strategies to elucidate signaling through cell adhesion molecules, includes experimental techniques to investigate integrin functions at organismal levels in a physiological context, and showcases the most promising methods and technologies for the development of novel therapeutics and diagnostics. Written in the successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Both experts and non-experts in the scientific community who wish to study cell adhesion molecules and diagnostics will find Integrin and Cell Adhesion Molecules: Methods and Protocols authoritative, easily accessible, and vastly informative.

目次

Part I: Basic Protocols for the Study of Integrin and Related Cell Adhesion Molecule Functionality In Vitro 1. Overview: Assays for Studying Integrin-Dependent Cell Adhesion Alexandre Chigaev and Larry A. Sklar 2. Cell Adhesion Assays Gabriele Weitz-Schmidt and Stephanie Chreng 3. Real Time Analysis of Integrin-dependent Transendothelial Migration and Integrin-independent Interstitial Motility of Leukocytes Ziv Shulman and Ronen Alon 4. Lentiviral Gene Transfer Method to Study Integrin Function in T Lymphocytes Daliya Banerjee and Motomu Shimaoka 5. Surface Plasmon Resonance Biosensing in Studies of the Binding between b2 Integrin I Domains and their Ligands Thomas Vorup-Jensen 6. Cell-Free Ligand-Binding Assays for Integrin LFA-1 Koichi Yuki Part II: Structural Biology Approaches for Studying Integrins and Related Cell Adhesion Molecules 7. Overview: Structural Biology of Integrins Guanyuan Fu, Wei Wang, and Bing-Hao Luo 8. Protein Expression and Purification of Integrin I Domains and IgSF Ligands for Crystallography Hongmin Zhang and Jia-huai Wang 9. Electron Microscopic Imaging of Integrin Kenji Iwasaki 10. An NMR Method to Study Protein-Protein Interactions Noritaka Nishida and Ichio Shimada 11. Single Molecule Methods to Study Cell Adhesion Molecules Joonil Seog Part III: Imaging for Investigating Cell Adhesion and Migration 12. Overview: Imaging in the Study of Integrins Christopher V. Carman 13. Live Imaging of LFA-1-dependent T Cell Motility and Stop Signals Andrew J. Wiemer, Sarah Wernimont, and Anna Huttenlocher 14. Monitoring Integrin Activation by Fluorescence Resonance Energy Transfer Craig T. Lefort, Young-min Hyun, and Minsoo Kim 15. High-resolution Fluorescence Microscopy to Study Transendothelial Migration Christopher V. Carman 16. Multiphoton Intravital Microscopy to Study Lymphocyte Motility in Lymph Nodes Thomas T. Murooka and Thorsten R. Mempel Part IV: Signaling through Cell Adhesion Molecules 17. Overview of Integrin Signaling in the Immune System Tatsuo Kinashi 18. Rap1 and Integrin Inside-out Signaling Koko Katagiri and Tatsuo Kinashi 19. Isolation of Focal Adhesion Proteins for Biochemical and Proteomic Analysis Jean-Cheng Kuo, Xuemei Han, John R. Yates III, and Clare M. Waterman 20. Talin and Signaling Through Integrins Mohamed Bouaouina, David S. Harburger, and David A. Calderwood 21. Proteomics Method for Identification of Pseudopodium Phosphotyrosine Proteins Yingchun Wang and Richard L. Klemke Part V: Cell Adhesion and Migration at Organismal Levels in a Physiological Context 22. Overview: Studying Integrins In Vivo Clifford A. Lowell and Tanya N. Mayadas 23. A Method for the Generation of Conditional Gene-Targeted Mice Masahiro Yamamoto and Kiyoshi Takeda 24. T Cell Homing to the Gut Mucosa: General Concepts and Methodological Considerations Jaime De Calisto, Eduardo J. Villablanca, Sen Wang, Maria R. Bono, Mario Rosemblatt, and J. Rodrigo Mora 25. In Vivo Quantitative Proteomics: the SILAC-Mouse Sara Zanivan, Marcus Krueger, and Matthias Mann 26. Analysis of Chemotaxis in Dictyostelium Huaqing Cai, Chuan-Hsiang Huang, Peter N. Devreotes, and Miho Iijima Part VI: Methods and Technologies towards Novel Therapeutics and Diagnostics Targeting Integrins 27. Integrins in Tumor Angiogenesis & Lymphangiogenesis Philippe Foubert and Judith A. Varner 28. PET-Radioimmunodetection of Integrins: Imaging Acute Colitis Using a 64Cu-Labeled Anti-b7 Integrin Antibody Jason L.J. Dearling and Alan B. Packard 29. Integrin-targeted Nanoparticles for siRNA Delivery Noa Ben-Arie, Ranit Kedmi, and Dan Peer 30. Humanized Mice for Studying Human Leukocyte Integrins In Vivo Sang-Soo Kim, Priti Kumar, and Premlata Shankar

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詳細情報

• NII書誌ID(NCID): BB07991957
• ISBN:
  • 9781617791659
• LCCN: 2011930756
• 出版国コード: us
• タイトル言語コード: eng
• 本文言語コード: eng
• 出版地: New York
• ページ数/冊数: xvi, 529 p.
• 大きさ: 27 cm
• 分類:
  • DC22 : 571.74
• 件名:
  • LCSH : Integrins
  • LCSH : Cell adhesion
• 親書誌ID:
  • BA10794673
  • BA88873632