Formalin-fixed paraffin-embedded tissues : methods and protocols
著者
書誌事項
Formalin-fixed paraffin-embedded tissues : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, 724）（Springer protocols）
Humana, c2011
- hbk.
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注記
Formerly CIP Uk
Includes bibliographical references and index
内容説明・目次
内容説明
Presenting an area of research that intersects with and integrates diverse disciplines, including genomics, epigenetics, proteomics, and cellular biology, among others, Formalin-Fixed Paraffin-Embedded Tissues: Methods and Protocols collects contributions from expert researchers in order to provide practical guidelines to this complex study. Compiled in order to provide researchers with up-to-date methodological information pertaining to the utilization of genomic, transcriptomic, and proteomic data in diagnosis, prognosis, and tailored therapy, the ultimate aim of this volume is to decipher diseases at a molecular level. Divided into multiple convenient chapters, this detailed book covers various techniques to construct and utilize tissue arrays, it also provides detailed protocols in immunohistochemistry, immunofluorescence, fluorescent and chromogenic in situ hybridization, and ultimately introduces protocols for FFPET microdissection and nucleic acids extraction for their utilization in advanced techniques such as microarray CGH, DNA methylation and pyrosequencing. The volume also discusses FFPET research from an ethical standpoint and concludes with a chapter on novel tissue fixative. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and accessible, Formalin-Fixed Paraffin-Embedded Tissues: Methods and Protocols serves as a practical guide for scientists of all backgrounds and aims to convey the appropriate sense of fascination associated with this vital field of research.
目次
1. Regulatory and Ethical Issues on the Utilization of FFPE Tissues in Research
Catherine M. With, David L. Evers, and Jeffrey T. Mason
2. Tissue Microarrays: Construction and Uses
Carol B. Fowler, Yan-Gao Man, Shimin Zhang, Timothy J. O'Leary, Jeffrey T. Mason, and Robert E. Cunningham
3. Standardization in Immunohistology
Anthony S-Y Leong and Trishe Y-M Leong
4. Multiple Immunofluorescence Labeling of Formalin-fixed Paraffin-embedded (FFPE) Tissue
David Robertson and Clare M. Isacke
5. Microwaves for Chromogenic-in-situ-hybridization
Anthony S-Y Leong and Zenobia Haffajee
6. Automated Analysis of FISH-stained HER2/neu Samples with Metafer
Christian Schunck and Eiman Mohammad
7. Laser Capture Microdissection (LCM) of FFPE Tissue Sections
Bridging the Gap between Microscopy and Molecular Analysis
Renate Burgemeister
8. Nucleic Acids Extraction from Laser Microdissected FFPE Tissue Sections
Renate Burgemeister
9. Microarray-based CGH and Copy Number Analysis of FFPE Samples
Fahd Al-Mulla
10. Microarray Profiling of DNA Extracted from FFPE Tissues using SNP6.0 Affymetrix Platform
Marianne Tuefferd, An de Bondt, Ilse Van den Wyngaert, Willem Talloen, and Hinrich Goehlmann
11. Whole Genome Amplification of DNA Extracted From FFPE Tissues
Mira Bosso and Fahd Al-Mulla
12. Pyrosequencing of DNA Extracted from Formalin-Fixed Paraffin-embedded Tissue
Brendan Doyle, Ciaran O'Riain, and Kim Appleton
13. Analysis of DNA Methylation in FFPE Tissues using the MethyLight Technology
Ashraf Dallol, Waleed Al- Ali, Amina Al-Shaibani, and Fahd Al-Mulla
14. RT-PCR Gene Expression Profiling of RNA from Paraffin-Embedded Tissues Prepared Using a Range of Different Fixatives and Conditions
Mei-Lan Liu, Jennie Jeong, Ranjana Ambannavar, Carl Millward, Frederick Baehner, Chithra Sangli, Debjani Dutta, Mylan Pho, Anhthu Nguyen, and Maureen T. Cronin
15. RT-PCR-Based Gene Expression Profiling for Cancer Biomarker Discovery from Fixed Paraffin Embedded Tissues
Aaron Scott, Ranjana Ambannavar, Jennie Jeong, Mei-Lan Liu and Maureen T. Cronin
16. microRNA Isolation from Formalin-Fixed Paraffin-Embedded Tissues
Aihua Liu and Xiaowei Xu
17. Gene Expression Profiling of RNA Extracted from FFPE tissues: NuGEN Technologies' Whole-Transcriptome Amplification System
Leah Turner, Joe Don Heath, and Nurith Kurn
18. Protein Mass Spectrometry Applications on FFPE Tissue Sections
Carol B. Fowler, Timothy J. O'Leary, and Jeffrey T. Mason
19. An Alternative Fixative to Formalin Fixation for Molecular Applications: the RCL2a-CS100 Approach
Amelie Denouel, Florence Boissiere-Michot, Philippe Rochaix, Frederic Bibeau, Nathalie Boulle
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