The plant cell wall : methods and protocols
著者
書誌事項
The plant cell wall : methods and protocols
(Methods in molecular biology / John M. Walker, series editor, v. 715)(Springer protocols)
Humana Press, c2011
- : hbk
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
The cell wall and its constituent polysaccharides and proteins control nearly all plant-based biological and biophysical processes. Understanding the cell wall is, therefore, not only fundamental to the plant sciences but is also pertinent to aspects of human and animal nutrition and health as well as plant-microbe and plant-animal interactions. In The Plant Cell Wall: Methods and Protocols, experts in the field describe detailed methods which are currently being applied to investigate the many aspects of the plant cell wall including its structure, biochemical composition, and metabolism. The book delves into a range of techniques involving plant tissue culture, which can be applied to investigating cell wall structure and metabolism, methods directed towards structural analysis and occurrence of carbohydrates, the development and use of microscopy-based tools and techniques, procedures which measure the physical properties of the wall, and methods based on the application of molecular genetic approaches. Written in the successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, The Plant Cell Wall: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies in an effort to further our knowledge of this essential cellular feature.
目次
1. Plant Tissue Cultures
Anna Karkoenen, Arja Santanen, Kuninori Iwamoto, and Hiroo Fukuda
2. Computerized Molecular Modelling of Carbohydrates
Alfred D. French and Glenn P. Johnson
3. Oligosaccharide Mass Profiling (OLIMP) of Cell Wall Polysaccharides by MALDI-TOF/MS
Markus Gunl, Florian Kraemer, and Markus Pauly
4. High-Voltage Paper Electrophoresis (HVPE) of Cell-Wall Building Blocks and Their Metabolic Precursors
Stephen C. Fry
5. Carbohydrate Gel Electrophoresis
Florence Goubet, Paul Dupree, and Katja Salomon Johansen
6. Capillary Electrophoresis with Detection by Laser-Induced Fluorescence
Andrew Mort and Xiangmei Wu
7. Monoclonal antibodies, Carbohydrate-Binding Modules, and the Detection of Polysaccharides in Plant Cell Walls
Cecile Herve, Susan E. Marcus, and J. Paul Knox
8. Screening and Characterization of Plant Cell Walls Using Carbohydrate Microarrays
Iben Sorensen and William G.T. Willats
9. Electron Tomography and Immunogold Labelling as Tools to Analyze de novo Assembly of Plant Cell Walls
Marisa S. Otegui
10. Analyzing Cellulose Biosynthesis with Confocal Microscopy
Meera Nair and Seth DeBolt
11. Visual Mapping of Cell Wall Biosynthesis
Yumiko Sakuragi, Morten H.H. Norholm, and Henrik V. Scheller
12. Atomic Force Microscopy of Plant Cell Walls
Andrew R. Kirby
13. Using Solid-State 13C NMR Spectroscopy to Study the Molecular Organization of Primary Plant Cell Walls
Tracey J. Bootten, Philip J. Harris, Laurence D. Melton, and Roger H. Newman
14. Formation of Cellulose-Based Composites with Hemicelluloses and Pectins Using Gluconacetobacter Fermentation
Deirdre Mikkelsen and Michael J. Gidley
15. Structural Proteins of the Primary Cell Wall: Extraction, Purification, and Analysis
Derek T.A. Lamport, Li Tan, and Marcia J. Kieliszewski
16. New Insights into the Control of Cell Growth
Claudia Blaukopf, Matthaus Z. Krol, and Georg J. Seifert
17. Extraction and Detection of Arabinogalactan Proteins
Zoe A. Popper
18. Characterization of the Plant Cell Wall Proteome Using High Throughput Screens
Sang-Jik Lee and Jocelyn K.C. Rose
19. Knocking Out the Wall: Protocols for Gene Targeting in Physcomitrella patens
Alison W. Roberts, Christos S. Dimos, Michael J. Budziszek, Jr., Chessa A. Goss, and Virginia Lai
20. Measuring In vitro Extensibility of Growing Plant Cell Walls
Daniel J. Cosgrove
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