Proteoglycans : methods and protocols
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Bibliographic Information
Proteoglycans : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, 836）（Springer protocols）
Humana Press, c2012
- : [hardcover]
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Note
Includes bibliographical references and index
"Humana Press is part of Springer Science + Business Media"--T.p. verso
Description and Table of Contents
Description
Now recognized as a reservoir for growth factors and cytokines modulating cell activation status and turnover, proteoglycans have stimulated great amount of interest and research. In Proteoglycans: Methods and Protocols, experts in the field examine issues of basic concepts and up-to-date analysis methods for proteoglycan and glycosaminoglycan (GAG) at the protein and saccharide levels. The volume continues with the multifunctional aspect of proteoglycans, highlighted through three relevant examples of proteoglycans with highly different strucutures: serglycin, aggrecan, and heparin sulphate proteoglycans. The final chapter describes proteoglycan involvement in the pathogenesis of various disorders and their potential therapeutic value in osteo-articular diseases. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and accessible, Proteoglycans: Methods and Protocols is an ideal guide for scientists attempting to pursue further research in this vital and complex area of study.
Table of Contents
Part I: Proteoglycans
1. Proteoglycans: Gene Cloning
Mauricio Cortes, James R. Mensch, Miriam Domowicz, and Nancy B. Schwartz
2. Proteoglycan: Site Mapping and Site-Directed Mutagenesis
Fred K. Hagen
3. Mapping of the Wnt/ -Catenin/TCF Response Elements in the Human Versican Promoter
Maziar Rahmani, Jon M. Carthy, and Bruce M. McManus
4. Gene Silencing in Mouse Embryonic Stem Cells
Norihiko Sasaki and Shoko Nishihara
5. A Novel Strategy for a Splice-Variant Selective Gene Ablation: The Example of the Versican V0/V2 Knockout
Maria T. Dours-Zimmermann and Dieter R. Zimmermann
6. Detection of Neurocan in Cerebrospinal Fluid
Uwe Rauch
Part II: Glycosaminoglycans Analysis
7. Glycosaminoglycan Chain Analysis and Characterization (Glycosylation/Epimerization)
Shuji Mizumoto and Kazuyuki Sugahara
8. Characterization of Glycosaminoglycans by Tandem Vibrational Microspectroscopy and Multivariate Data Analysis
Nathalie Mainreck, Stephane Brezillon, Ganesh D. Sockalingum, Francois-Xavier Maquart, Michel Manfait, and Yanusz Wegrowski
9. Glycosaminoglycans: Oligosaccharide Analysis by Liquid Chromatography/Specific Labeling
Derek J. Langelsay, Christopher J. Jones, Szabolcs Beni, and Cynthia K. Larive
10. Brain Chondroitin/Dermatan Sulfate, from Cerebral Tissue to Fine Structure: Extraction, Preparation, and Fully Automated Chip-Electrospray Mass Spectrometric Analysis
Alina D. Zamfir, Corina Flangea, Alina Serb, Eugen Sisu, Leon Zagrean, Andreas Rizzi, and Daniela G. Seidler
11. Use of Neutrons Reveal the Dynamics of Cell Surface Glycosaminoglycans
Marion Jasnin
12. Following Protein-Glycosaminoglycan (GAG) Polysaccharide Interactions with Differential Scanning Fluorimetry (DSF)
Katarzyna A. Uniewicz, Alessandro Ori, Timothy R. Rudd, Marco Guerrini, Mark C. Wilkinson, David G. Fernig, and Edwin A. Yates
13. In Vivo Scintigraphic Imaging of Proteoglycans
Elisabeth Miot-Noirault, Aurelien Vidal, Philippe Auzeloux, Caroline Peyrode, Jean-Claude Madelmont, and Jean-Michel Chezal
Part III: PGs: Multifunctional Cell Regulators
14. Serglycin: The Master of the Mast Cell
Elin Roennberg and Gunnar Pejler
15. Analysis of Aggrecan Catabolism by Immunoblotting and Immunohistochemistry
Peter J. Roughley and John S. Mort
16. Heparan Sulfate Proteoglycans as Multifunctional Cell Regulators: Cell Surface Receptors
Jin-ping Li and Dorothe Spillmann
Part IV: Proteoglycans Involvement in Pathophysiology
17. Models for Studies of Proteoglycans in Kidney Pathophysiology
Scott J. Harvey
18. Lumican Promotes Corneal Epithelial Wound Healing
Chia-Yang Liu and Winston Whei-Yang Kao
19. Shedding of Cell Membrane-Bound Proteoglycans
Eon Jeong Nam and Pyong Woo Park
20. Modulatory Effects of Proteoglycans on Proteinase Activities
Steven Georges, Dominique Heymann, and Marc Padrines
21. Proteoglycans and Osteolysis
Marc Baud'Huin, Celine Charrier, Gwenola Bougras, Regis Brion, Frederic Lezot, Marc Padrines, and Dominique Heymann
22. Proteoglycans and Cartilage Repair
Mohamed Ouzzine, Narayanan Venkatesan, and Sylvie Fournel-Gigleux
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