Plant fungal pathogens : methods and protocols

著者

• Bolton, Melvin D. (Melvin Dennis)
• Thomma, Bart P. H. J.

書誌事項

Plant fungal pathogens : methods and protocols

edited by Melvin D. Bolton, Bart P. H. J. Thomma

（Methods in molecular biology / John M. Walker, series editor, 835）（Springer protocols）

Humana Press, c2012

注記

Includes bibliographical references and index

内容説明・目次

内容説明

Over the course of evolution, fungi have adapted to occupy specific niches, from symbiotically inhabiting the flora of the intestinal tract of mammals to saprophytic growth on leaf litter resting on the forest floor. In Plant Fungal Pathogens: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study fungal plant pathogens. These include methods and techniques for model systems such as Arabidopsis thaliana as well as crop plants, aspects of fungal biology, genome annotation, next-generation sequencing, and fungal transformation and molecular tools for disease and/or pathogen quantification that are critical for revealing the role for a fungal gene of interest in disease development. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Fungal Pathogens: Methods and Protocols seeks to aid scientists in the further study in current techniques that cover a wide-range of methods to study molecular aspects of pathogenesis.

目次

1. Fungal ABC Transporter Deletion and Localization AnalysisAndriy Kovalchuk, Stefan Weber, Jeroen G. Nijland, Roel A.L. Bovenberg, and Arnold J.M. Driessen2. Targeted Gene Replacement in Fungal Pathogens via Agrobacterium tumefaciens Mediated TransformationRasmus John Normand Frandsen, Mette Frandsen, Henriette Giese 3. Activity-based Protein Profiling of Infected Plants Farnusch Kaschani, Christian Gu, Renier A.L. van der Hoorn 4. The use of Agroinfiltration for Transient Expression of Plant Resistance and Fungal Effector Proteins in Nicotiana benthamiana Leaves Lisong Ma, Ewa Lukasik, Fleur Gawehns and Frank L.W. Takken 5. Proteomic Techniques for Plant-Funal Interactions Delphine Vincent, Kar-Chun Tan, Liam Cassidy, Peter Solomon and Richard Oliver 6. Identification of HR-inducing cDNAs from Plant Pathogens via a Gateway (R)-Compatible Binary Potato Virus X-Expression Vector H. Peter van Esse 7. Freeze-Fracture of Infected Plant Leaves in Ethanol for Scanning Electron Microscopic Study of Fungal Pathogens Jayma A. Moore and Scott A. Payne 8. Real-time PCR for the Quantification of Fungi in planta Steven J. Klosterman 9. Using Non-homologous End-joining-deficient Strains for Functional Gene Analyses in Filamentous Fungi Mark Arentshorst, Arthur F.J. Ram, Vera Meyer 10. Atomic Force Microscopy (AFM): A Tool for Studying Biophysical Surface Properties Underpinning Fungal Interactions with Plants and Substrates Elizabeth Adams,Danielle Emerson, Sean Croker, Hye-Seon Kim, Shannon Modla, Seogchan Kang, Kirk Czymmek 11. Use of the Yeast Two-Hybrid System to Identify Targets of Fungal Effectors Shunwen Lu 12. Characterization of Plant - Fungal Interactions Involving Necrotrophic Effector-Producing Plant Pathogens Timothy L. Friesen and Justin D. Faris 13.Heterologous Production of Fungal Effectors in Pichia pastoris Anja Kombrink 14. The Application of Laser Microdissection to Profiling Fungal Pathogen Gene Expression in planta Wei-Hua Tang, Yan Zhang, and Jon Duvick 15. Metabolomics Protocols for Filamentous Fungi Joel P.A. Gummer, Christian Krill, Lauren Du Fall, Ormonde D.C. Waters, Robert D. Trengove, Richard P. Oliver and Peter S. Solomon 16. Targeted Gene Replacement in Fungi using a Split-Marker Approach Rubella S. Goswami 17.Massively Parallel Sequencing Technology in Pathogenic Microbes Sucheta Tripathy and Rays H.Y. Jiang 18.Confocal Microscopy in Plant Pathogen Interactions Adrienne R. Hardham 19.The use of Open Source Bioinformatics Tools to Dissect Transcriptomic Data Benjamin M. Nitsche, Arthur F.J. Ram, Vera Meyer 20.Population Biology of Fungal Plant Pathogens Zahi K. Atallah and Krishna V. Subbarao 21.Polyethylene Glycol (PEG)-mediated Transformation in Filamentous Fungal Pathogens Zhaohui Liu and Timothy L. Friesen 22.In vitro Induction of Infection-related Hyphal Structures in Plant Pathogenic Fungi W.R. Rittenour, S.D. Harris 23.Fungicide Resistance Assays for Fungal Plant Pathogens Gary A. Secor and Viviana V. Rivera 24.Identification of Lipid-binding Effectors Shiv D. Kale and Brett M. Tyler 25. In silico Identification and Characterization of Effector Catalogs Ronnie de Jonge 26. Horizontal Transfer of Supernumerary Chromosomes in Fungi H. Charlotte van der Does and Martijn Rep 27. The Induction of Mycotoxins by Trichothecene Producing Fusarium Species Rohan Lowe, Melanie Jubault, Gail Canning, Martin Urban and Kim E. Hammond-Kosack 28. Identifying Genes in Fusarium verticillioides Through Forward and Reverse Genetics J.B. Ridenour, R.L. Hirsch, and B.H. Bluhm 29.Assessment of Autophagosome Formation by Transmission Electron Microscopy (TEM) Marina Nadal and Scott E. Gold 30. Fungal Plant Pathogen Detection in Plant and Soil Samples using DNA Macroarrays B Lievens, A Juste and K A Willems 31. Random Insertional Mutagenesis in Fungal Genomes to Identify Virulence Factors Parthasarathy Santhanam 32. A Yeast-Secretion Trap Assay for Identification of Secreted Proteins from Eukaryotic Phytopathogens and their Plant Hosts Sang-Jik Lee and Jocelyn K.C. Rose 33. Comparing Fungal Genomes: Insight into Functional and Evolutionary Processes Eva H. Stukenbrock and Julien Y. Dutheil 34.Multi-gene Phylogenetic Analyses to Delimit New Species in Fungal Plant Pathogens Tara L. Rintoul , Quinn A. Eggertson , and C. Andre Levesque 35.MAP Kinase Phosphorylation and cAMP Assessment in Fungi Rahim Mehrabi, Sarrah Ben M'Barek, Abbas Saidi, Masoud Abrinbana, Mostafa Aghaee, Pierre J. G. M. de Wit and Gert H. J. Kema 36.A One-step Affinity-purification Protocol to Purify NB-LRR Immune Receptors from Plants that Mediate Resistance to Fungal Pathogens Wladimir I.L. Tameling 37.Karyotyping Methods for Fungi Rahim Mehrabi, Masatoki Taga, Mostafa Aghaee, Pierre J. G. M. de Wit, Gert H. J. Kema 38.Isolation of Apoplastic Fluid from Leaf Tissue by the Vacuum Infiltration-Centrifugation Technique Matthieu H.A.J. Joosten 39.Gene Cloning using Degenerate Primers and Genome Walking Javier A. Delgado, Steven Meinhardt, Samuel G. Markell and Rubella S. Goswami 40.Construction of Hairpin RNA Expressing Vectors for RNA-mediated Gene Silencing in Fungi Shaobin Zhong, Yueqiang Leng, Melvin D. Bolton 41.An Unbiased Method for the Quantitation of Disease Phenotypes using a Custom-built Macro Plugin for the Program ImageJ Ahmed Abd-El-Haliem

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詳細情報

• NII書誌ID(NCID): BB09104469
• ISBN:
  • 9781617795008
• LCCN: 2011943884
• 出版国コード: us
• タイトル言語コード: eng
• 本文言語コード: eng
• 出版地: New York
• ページ数/冊数: xvi, 648 p.
• 大きさ: 27 cm
• 分類:
  • LCC : SB732.7
• 件名:
  • LCSH : Plant-pathogen relationships
  • LCSH : Fungal diseases of plants
• 親書誌ID:
  • BA10794673
  • BA88873632