Influenza virus : methods and protocols
著者
書誌事項
Influenza virus : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, 865）（Springer protocols）
Humana Press, c2012
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内容説明・目次
内容説明
Reports of influenza-like illnesses date back to the Middle Ages, and outbreaks of influenza likely afflicted humans long before that. Over the last half century, influenza virus research has led to the development of two classes of antivirals - ion channel and neuraminidase inhibitors. Recently, a method of the artificial generation of an influenza virus was established. This system has been instrumental in the development of novel influenza vaccines and in the understanding of viral pathogenicity and the functions of viral proteins. Influenza Virus: Methods and Protocols summarizes the current techniques that have made this progress possible, ranging from protocols for virus isolation, growth, and subtyping to procedures for the efficient generation of any influenza virus. Written in the successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Influenza Virus: Methods and Protocols seeks to serve both professionals and novices with the techniques used in numerous laboratories around the world that are, thus, the building blocks that underpin almost all influenza virus research.
目次
1. Influenza Viruses - An Introduction
Yoshihiro Kawaoka and Gabriele Neumann
2. Influenza Virus Isolation
Scott Krauss, David Walker, and Robert G. Webster
3. Influenza Virus Titration, Antigenic Characterization, and Serological Methods for Antibody Detection
Alexander Klimov, Amanda Balish, Vic Veguilla, Hong Sun, Jarad Schiffer, Xiuhua Lu, Jacqueline M. Katz, and Kathy Hancock
4. Diagnosis of Influenza Virus
Kirsten St. George
5. Solid-phase Assays of Receptor-binding Specificity
Mikhail N. Matrosovich and Alexandra S. Gambaryan
6. The Chemiluminescent Neuraminidase Inhibition Assay: A Functional Method for Detection of Influenza Virus Resistance to the Neuraminidase Inhibitors
Margaret Okomo-Adhiambo, Aeron C. Hurt, and Larisa V. Gubareva
7. The Fluorescence Neuraminidase Inhibition Assay: A Functional Method for Detection of Influenza Virus Resistance to the Neuraminidase Inhibitors
Aeron C. Hurt, Margaret Okomo-Adhiambo, and Larisa V. Gubareva
8. Animal Models
Edwin J.B. Veldhuis Kroeze, Thijs Kuiken, and Albert D.M.E. Osterhaus
9. Influenza Virus Surveillance, Vaccine Strain Selection and Manufacture
Klaus Stoehr, Doris Bucher, Tony Colgate, and John Wood
10. Genetic Engineering of Live Attenuated Influenza Viruses
Hong Jin, Zhongying Chen, Jonathan Liu, and George Kemble
11. Influenza A Virus Molecular Virology Techniques
Bin Zhou and David E. Wentworth
12. Reverse Genetics of Influenza Viruses
Gabriele Neumann, Makoto Ozawa, and Yoshihiro Kawaoka
13. Genetic Analysis
Gavin J.D. Smith, Justin Bahl, and Vijaykrishna Dhanasekaran
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