Correlative light and electron microscopy

著者

書誌事項

Correlative light and electron microscopy

edited by Thomas Müller-Reichert, Paul Verkade

（Methods in cell biology / edited by David M. Prescott, v. 111, 124, 140）

Academic Press, 2012-

  • [1]
  • 2
  • 3

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注記

Includes bibliographical references and index

[1]: xix, 404 p., [40] p. of plates : ill. (some col.) ; 25 cm

2: xviii, 442 p., [32] p. of plates : ill. (some col.) ; 25 cm

3: xvii, 352 p. : ill. (some col.) ; 25 cm

内容説明・目次

巻冊次

[1] ISBN 9780124160262

内容説明

The combination of electron microscopy with transmitted light microscopy (termed correlative light and electron microscopy; CLEM) has been employed for decades to generate molecular identification that can be visualized by a dark, electron-dense precipitate. This new volume of Methods in Cell Biology covers many areas of CLEM, including a brief history and overview on CLEM methods, imaging of intermediate stages of meiotic spindle assembly in C. elegans embryos using CLEM, and capturing endocytic segregation events with HPF-CLEM.

目次

Imaging Fluorescently Labelled Complexes by Means of Multidimensional Correlative Light and Transmission Electron Microscopy: Practical Considerations Visualising Live Dynamics and Ultrastructure of Intracellular Organelles with Pre-Embedding Correlative Light-Electron Microscopy Correlative Fluorescence and Transmission Electron Microscopy in Tissues Correlative Light and Electron Microscopy in Parasite Research Labeling of Ultrathin Resin Sections for Correlative Light and Electron Microscopy 3D HDO-CLEM: Cellular Compartment Analysis by Correlative Light-Electron Microscopy on Cryosection Correlative Light and Electron Microscopy of GFP Picking Faces Out of a Crowd: Genetic Labels for Identification of Proteins in Correlated Light and Electron Microscopy Imaging Correlated Light Microscopy and Electron Microscopy (CLEM): Search... and Find! Capturing Endocytic Segregation Events with HPF-CLEM Targeted Ultramicrotomy: a Valuable Tool for Correlated Light and Electron Microscopy of Small Model Organisms Correlative Light and Electron Microscopy of Intermediate Stages of Meiotic Spindle Assembly in the Early C. Elegans Embryo Precise, Correlated Fluorescence Microscopy and Electron Tomography of Lowicryl Sections Using Fluorescent Fiducial Markers Integrative Approaches for Cellular Cryo-Electron Tomography:Correlative Imaging and Focused Ion Beam Micromachining Visualizing Proteins in Electron Micrographs at Nanometer Resolution Atmospheric Scanning Electron Microscope for Correlative Microscopy Bridging Microscopes: 3D Correlative Light and Electron Microscopy of Complex Biological Structures Correlative Light and Volume EM: Using Focused Ion Beam Scanning Electron Microscopy to Image Transient Events in Model Organisms
巻冊次

2 ISBN 9780128010754

内容説明

This new volume of Methods in Cell Biology looks at methods for analyzing correlative light and electron microscopy (CLEM). With CLEM, people try to combine the advantages of both worlds, i.e. the dynamics information obtained by light microscopy and the ultrastructure as provided by electron microscopy. This volume contains the latest techniques on correlative microscopy showing that combining two imaging modalities provides more than each technique alone. Most importantly it includes the essential protocols, including tips, tricks and images for you to repeat these exciting techniques in your own lab. With cutting-edge material, this comprehensive collection is intended to guide researchers for years to come.

目次

1. Retracing in Correlative Light Electron Microscopy - Where is My Object of Interest? 2. Fluorescing the Electron: Strategies in Correlative Experimental Design 3. Metallothioneins for Correlative Light and Electron Microscopy 4. Correlative Light Microscopy and Electron Tomography to Study Von Willebrand Factor Exocytosis From Vascular Endothelial Cells 5. A Simple Procedure to Analyze Positions of Interest in Infectious Cell Cultures by Correlative Light and Electron Microscopy 6. Correlative Light and Electron Microscopy for a Free-Floating Spindle in Xenopuslaevis Egg Extracts 7. Combining Wide-field Super-resolution Microscopy and Electron Tomography: Rendering Nanoscopic Correlative Arrays on Subcellular Architecture 8. Correlative Cryo-fluorescence and Cryo-soft X-Ray Tomography of Adherent Cells at European Synchrotrons 9. Critical Step-by-step Approaches Toward Correlative Fluorescence/Soft X-Ray Cryo-Microscopy of Adherent Mammalian Cells 10. Correlative Cryo-fluorescence Light Microscopy and Cryo-electron Tomography of Streptomyces 11. Immuno Correlative Light and Electron Microscopy on Tokuyasu Cryosections 12. Step by Step Manipulation of the Cryocapsule with HPM High Pressure Freezers 13. Live-cell CLEM of Sub-cellular Targets: An Optimized Procedure for Polymer Based Imaging Substrates 14. Correlative Fluorescence- and Scanning Transmission Electron Microscopy of Quantum Dot Labeled Proteins on Whole Cells in Liquid 15. The Use of a Laser for Correlating Light and Electron Microscopic Images in Thick Tissue Specimens 16. Correlative In Vivo 2-photon Imaging and Focused Ion Beam Scanning Electron Microscopy: 3D Analysis of Neuronal Ultrastructure 17. Integrated Light and Scanning Electron Microscopy of GFP-Expressing Cells 18. Correlation of the Same Fields Imaged in the TEM, Confocal, LM and MicroCT by Image Registration: From Specimen Preparation to Displaying a Final Composite Image
巻冊次

3 ISBN 9780128099759

内容説明

Correlative Light and Electron Microscopy III, Volume 140, a new volume in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Topics discussed in this new release include Millisecond time-resolved CLEM, Super resolution LM und SEM of high-pressure frozen C. elegans, Preservation fluorescence, super res CLEM, APEX in Tissue, Corrsight mit IBIDI flowthrough chamber, Correlative Light Atomic Force Electronic Microscopy (CLAFEM), Atmospheric EM CLEM, and High-precision correlation, amongst other topics. Chapters in this ongoing series deal with different approaches for analyzing the same specimen using more than one imaging technique. The strengths and application area of each is presented, with this volume exploring the aspects of sample preparation of diverse biological systems for different CLEM approaches.

目次

1. Millisecond time-resolved CLEM2. Super resolution LM und SEM of high-pressure frozen C. elegans3. Preservation fluorescence, super res CLEM4. Targeted ultramicrotomy5. CEMOVIS-CLEM6. APEX in Tissue7. Corrsight mit IBIDI flowthrough chamber8. Corrsight9. Correlative Light Atomic Force Electronic Microscopy (CLAFEM)10. Atmospheric EM CLEM11. Correlated Fluo Light SEM12. Near infrared branding + SBF SEM13. 4. Correlative MicroCT14. High-precision correlation15. CLEM acquisition16. ecCLEM mapping software

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  • Methods in cell biology

    edited by David M. Prescott

    Academic Press 1964-

    v. 1 , v. 2 , v. 3 , v. 4 , v. 5 , v. 6 , v. 7 , v. 8 , v. 9 , v. 10 , v. 11 , v. 12 , v. 13 , v. 14 , v. 15 , v. 20 , v. 21A , v. 21B , v. 24 , v. 25 , v. 26 , v. 27 , v. 28 , v. 29 , v. 30 , v. 31 , v. 32 , v. 33 , v. 34 , v. 35 , v. 36 , v. 40 , v. 44 , v. 54 , v. 72 , v. 73

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