Recombinant protein production in yeast : methods and protocols
著者
書誌事項
Recombinant protein production in yeast : methods and protocols
(Methods in molecular biology / John M. Walker, series editor, 866)(Springer protocols)
Humana Press, c2012
- : pbk
大学図書館所蔵 全5件
  青森
  岩手
  宮城
  秋田
  山形
  福島
  茨城
  栃木
  群馬
  埼玉
  千葉
  東京
  神奈川
  新潟
  富山
  石川
  福井
  山梨
  長野
  岐阜
  静岡
  愛知
  三重
  滋賀
  京都
  大阪
  兵庫
  奈良
  和歌山
  鳥取
  島根
  岡山
  広島
  山口
  徳島
  香川
  愛媛
  高知
  福岡
  佐賀
  長崎
  熊本
  大分
  宮崎
  鹿児島
  沖縄
  韓国
  中国
  タイ
  イギリス
  ドイツ
  スイス
  フランス
  ベルギー
  オランダ
  スウェーデン
  ノルウェー
  アメリカ
注記
Includes bibliographical references and index
内容説明・目次
内容説明
In the last few years, significant advances have been made in understanding how a yeast cell responds to the stress of producing a recombinant protein, and how this information can be used to engineer improved host strains. The molecular biology of the expression vector, through the choice of promoter, tag and codon optimization of the target gene, is also a key determinant of a high-yielding protein production experiment. Recombinant Protein Production in Yeast: Methods and Protocols examines the process of preparation of expression vectors, transformation to generate high-yielding clones, optimization of experimental conditions to maximize yields, scale-up to bioreactor formats and disruption of yeast cells to enable the isolation of the recombinant protein prior to purification. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Recombinant Protein Production in Yeast: Methods and Protocols, seeks to aid scientists in adopting yeast as a protein production host.
目次
1 Optimising Yeast as a Host for Recombinant Protein Production( review)
Nicklas Bonander and Roslyn M Bill
2 Which Yeast Species Shall I Choose? Saccharomyces cerevisiae versus Pichia pastoris (review)
Richard AJ Darby, Stephanie P Cartwright, Marvin V. Dilworth and Roslyn M Bill
3 Preparation of Pichia pastoris Expression Plasmids
Christel Logez, Fatima Alkhalfioui, Bernadette Byrne, Renaud Wagner
4 Preparation of Saccharomyces cerevisiae Expression Plasmids
David Drew and Hyun Kim
5 Codon Optimisation for Heterologous Gene Expression in Yeast
Kristina Hedfalk
6 Yeast Transformation to Generate High-yielding Clones
Mohammed Jamshad and Richard AJ Darby
7 Screening for High-yielding Pichia pastoris Clones: the Production of G Protein-Coupled Receptors as a Case Study
Shweta Singh, Adrien Gras, Cedric Fiez-Vandal, Magdalena Martinez, Renaud Wagner, Bernadette Byrne
8 Screening for High-yielding Saccharomyces cerevisiae Clones: Using a Green Fluorescent Protein Fusion Strategy in the Production of Membrane Proteins
David Drew and Hyun Kim
9 The Effect of Antifoam Addition on Protein Production Yields
Sarah J Routledge and Roslyn M Bill
10 Setting up a Bioreactor for Recombinant Protein Production in Yeast
Sarah J Routledge and Michelle Clare
11 The Implementation of a Design of Experiments Strategy to Increase Recombinant Protein Yields in Yeast (review)
Nagamani Bora, Zharain Bawa, Roslyn M. Bill and Martin D. B. Wilks
12 On-line Analysis and Process Control in Recombinant Protein Production (review)
Shane M. Palmer and Edmund R.S. Kunji
13 Monitoring the Biomass Accumulation of Recombinant Yeast Cultures: Off-line Estimations of Dry Cell Mass and Cell Counts
Shane Palmer and Edmund R S Kunji
14 On-line Monitoring of Biomass Accumulation in Recombinant Yeast Cultures
Shane Palmer and Edmund R S Kunji
15 Optimising P. pastoris Induction
Zharain Bawa and Richard AJ Darby
16 Optimizing S. cerevisiae Induction Regimes
David Drew and Hyun Kim
17 Large Scale Production of Membrane Proteins in Pichia pastoris: The Production of G Protein-coupled Receptors as a Case Study
Shweta Singh, Adrien Gras, Cedric Fiez-Vandal, Magdalena Martinez, Renaud Wagner and Bernadette Byrne
18 Large-scale Production of Membrane Proteins in Saccharomyces cerevisiae: Using a Green Fluorescent Protein Fusion Strategy in the Production of Membrane Proteins
David Drew and Hyun Kim
19 Large Scale Production of Secreted Proteins in Pichia pastoris
Nagamani Bora
20 Disruption of Yeast Cells to Isolate Recombinant Proteins
Mohammed Jamshad and Richard AJ Darby
「Nielsen BookData」 より