Malaria : methods and protocols

著者

    • Ménard, Robert

書誌事項

Malaria : methods and protocols

edited by Robert Ménard

(Methods in molecular biology / John M. Walker, series editor, 923)(Springer protocols)

Humana Press, c2012

2nd ed

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内容説明・目次

内容説明

Over the past ten years, many powerful new techniques have been developed that have dramatically changed malaria research. The second edition of Malaria: Methods and Protocols expands upon the previous edition with current, detailed techniques for laboratory research. With new chapters on parasite culture techniques, genome manipulation methods, 'omic' approaches, and techniques for studying the biology of the red blood cell and pre-erythrocytic stages of Plasmodium. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Malaria: Methods and Protocols, Second Edition, offers a comprehensive set of standard techniques for laboratory researchers.

目次

Part I. Parasite Culturing 1. In vitro Culturing P. falciparum Erythrocytic Stages Alexander G. Maier and Melanie Rug 2. Production of Plasmodium falciparum Gametocytes in vitro Kathryn Shaw Saliba and Marcelo Jacobs-Lorena 3. In vitro Differentiation of Plasmodium falciparum Gametocytes into Ookinetes Anil K. Ghosh and Marcelo Jacobs-Lorena 4. Ex vivo culture of Plasmodium Vivax and Plasmodium Cynomolgi and in vitro Culture of Plasmodium Knowlesi Blood Stages Anne-Marie Zeeman, Annemarie Voorberg-van der Wel, and Clemens H. M. Kocken 5. Laboratory Maintenance of Rodent Malaria Parasites Chandra Ramakrishnan, Michael J. Delves, Kalpana Lal, Andrew M. Blagborough, Geoffrey Butcher, Kenneth W. Baker, and Robert E. Sinden Part II. Genome Manipulation 6. Transfection of Plasmodium falciparum Melanie Rug and Alexander G. Maier 7. Transfection of Rodent Malaria Parasites Nisha Philip, Rachael Orr, and Andrew P. Waters 8. Recombination-mediated Genetic Engineering of Plasmodium berghei DNA Claudia Pfander, Burcu Anar, Mathieu Brochet, Julian C. Rayner, and Oliver Billker 9. Standardization in Generating and Reporting Genetically Modified Rodent Malaria Parasites: The RMgmDB Database Shahid M. Khan, Hans Kroeze, Blandine Franke-Fayard, and Chris J. Janse 10. Transient Transfection of Plasmodium vivax Blood-stage Parasites Cecilia P. Sanchez, Judith Pfahler, Hernando A del Portillo, and Michael Lanzer 11. Silencing of Genes and Alleles by RNAi in Anopheles gambiae Marina Lamacchia, John R. Clayton, Rui Wang-Sattler, Lars M. Steinmetz, Elena A. Levashina, and Stephanie A. Blandin Part III. 'OMIC' Techniques 12. Analysis of Variant Gene Family Expression by Quantitative PCR Sohini Sanyal, Thomas J. Templeton, and Cristina K. Moreira 13. DNA Microarray-based Genome-wide Analyses of Plasmodium Parasites Zbynek Bozdech, Sachel Mok, and Archna P. Gupta 14. Whole-genome Analysis of Plasmodium spp. Utilizing a New Agilent Technologies DNA Microarray Platform Heather J. Painter, Lindsey M. Altenhofen, Bjoern F.C. Kafsack and Manuel Llinas 15. Transcriptome Analysis using RNA-seq Wieteke A.M. Hoeijmakers, Richard Bartfai, and Hendrik G. Stunnenberg 16. Experimental Tools for the Study of Protein Phosphorylation in Plasmodium Dominique Dorin-Semblat, Andrew R. Bottrill, Lev Solyakov, Andrew Tobin, and Christian Doerig 17. Extraction of Hydrophilic Metabolites from Plasmodium falciparum-infected Erythrocytes for Metabolomic Analysis Kellen L. Olszewski and Manuel Llinas Part IV. Red Blood Cell Stages 18. Static and Dynamic Imaging of Erythrocyte Invasion and Early Intra-erythrocytic Development in Plasmodium falciparum David T. Riglar and Jake Baum 19. Flow Cytometry-based Methods for Measurement of Cytosolic Calcium and Surface Protein Expression in Plasmodium falciparum Merozoites Shailja Singh and Chetan E. Chitnis 20. Microsphiltration: a Microsphere Matrix to Explore Erythrocyte Deformability Catherine Lavazec, Guillaume Deplaine, Innocent Safeukui, Sylvie Perrot, Genevieve Milon, Odile Mercereau-Puijalon, Peter H. David, and Pierre Buffet 21. Atomic Force Microscopy of Plasmodium-infected Red Blood Cells for Detecting and Localizing Single Molecular Recognition Events Ang Li, Laurent Renia, Chwee Teck Lim, and Bruce Russell 22. Expressing Full-length Functional PfEMP1 Proteins in the HEK293 Expression System Anand Srivastava, Yves Durocher, and Benoit Gamain 23. Genome-wide Chromatin Immunoprecipitation-sequencing in Plasmodium Jose-Juan Lopez-Rubio, T. Nicolai Siegel, and Artur Scherf 24. In situ Fluorescence Visualization of Transcription Sites and Genomic Loci in Blood Stages of Plasmodium falciparum Liliana Mancio-Silva and Artur Scherf 25. Bioluminescence Imaging of P. berghei Schizont Sequestration in Rodents Joanna Braks, Elena Aime, Roberta Spaccapelo, Onny Klop, Chris J. Janse, and Blandine Franke-Fayard Part V. Pre-Erythrocytic Stages 26. Scoring Sporozoite Motility Allison F. Carey, Robert Menard, and Daniel Y. Bargieri 27. Quantification of Sporozoite Invasion, Migration and Development by Microscopy and Flow Cytometry Photini Sinnis, Patricia De La Vega, Alida Coppi, Urszula Krzych, and Maria M. Mota 28. Imaging Sporozoite Cell Traversal in the Liver of Mice Joana Tavares, Pauline Formaglio, Alexander Medvinsky, Robert Menard, and Rogerio Amino 29. Analysis of Liver Stage Development in, and Merozoite Release from Hepatocytes Rebecca R. Stanway, Jacqueline Schmuckli-Maurer, and Volker T. Heussler 30. Quantitative Analysis of Plasmodium berghei Liver Stages by Bioluminescence Imaging Takeshi Annoura, Severine Chevalley, Chris J. Janse, Blandine Franke-Fayard, and Shahid M. Khan Part VI. Immunity to Plasmodium 31. Activation of Human NK Cells by Plasmodium-infected Red Blood Cells Amir Horowitz and Eleanor M. Riley 32. Chimeric Parasites as Tools to Study Plasmodium Immunology and Assess Malaria Vaccines Ian Cockburn 33. Development and Use of TCR Transgenic Mice for Malaria Immunology Research Yun-Chi Chen and Fidel Zavala 34. Tracking the Total CD8 T Cell Response Following Whole Plasmodium Vaccination Katherine L. Doll, Noah S. Butler, and John T. Harty Part VII. Controlling Plasmodium Infection 35. Screening Inhibitors of P. berghei Blood Stages using Bioluminescent Reporter Parasites Jing-wen Lin, Mohammed Sajid, Jai Ramesar, Shahid M. Khan, Chris J. Janse, and Blandine Franke-Fayard 36. Screening and Evaluation of Inhibitors of Plasmodium falciparum Merozoite Egress and Invasion using Cytometry Anthony Bouillon, Olivier Gorgette, Odile Mercereau-Puijalon, and Jean-Christophe Barale 37. Induction of anti-Plasmodium Immunity Following Sub-patent Infection with Live Erythrocytic Stages and Drug Cure Danielle I. Stanisic and Michael F. Good 38. Vaccination using Radiation-or Genetically-Attenuated Live Sporozoites Ashley M Vaughan and Stefan H I Kappe 39. Vaccination using Normal Live Sporozoites Under Drug Treatment Laurent Renia, Anne Charlotte Gruner, Marjorie Mauduit, and Georges Snounou 40. Assessing Transmission Blockade in Plasmodium spp. Andrew M. Blagborough, Michael J. Delves, Chandra Ramakrishnan, Kalpana Lal, Geoffrey Butcher, and Robert E. Sinden 41. Mosquito Transgenic Technologies to Reduce Plasmodium Transmission Silke Fuchs, Tony Nolan, and Andrea Crisanti

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詳細情報

  • NII書誌ID(NCID)
    BB10373923
  • ISBN
    • 9781627030250
  • 出版国コード
    us
  • タイトル言語コード
    eng
  • 本文言語コード
    eng
  • 出版地
    New York
  • ページ数/冊数
    xv, 626 p.
  • 大きさ
    26 cm
  • 分類
  • 件名
  • 親書誌ID
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