Malaria : methods and protocols
著者
書誌事項
Malaria : methods and protocols
(Methods in molecular biology / John M. Walker, series editor, 923)(Springer protocols)
Humana Press, c2012
2nd ed
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内容説明・目次
内容説明
Over the past ten years, many powerful new techniques have been developed that have dramatically changed malaria research. The second edition of Malaria: Methods and Protocols expands upon the previous edition with current, detailed techniques for laboratory research. With new chapters on parasite culture techniques, genome manipulation methods, 'omic' approaches, and techniques for studying the biology of the red blood cell and pre-erythrocytic stages of Plasmodium. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Malaria: Methods and Protocols, Second Edition, offers a comprehensive set of standard techniques for laboratory researchers.
目次
Part I. Parasite Culturing
1. In vitro Culturing P. falciparum Erythrocytic Stages
Alexander G. Maier and Melanie Rug
2. Production of Plasmodium falciparum Gametocytes in vitro
Kathryn Shaw Saliba and Marcelo Jacobs-Lorena
3. In vitro Differentiation of Plasmodium falciparum Gametocytes into Ookinetes
Anil K. Ghosh and Marcelo Jacobs-Lorena
4. Ex vivo culture of Plasmodium Vivax and Plasmodium Cynomolgi and in vitro Culture of Plasmodium Knowlesi Blood Stages
Anne-Marie Zeeman, Annemarie Voorberg-van der Wel, and Clemens H. M. Kocken
5. Laboratory Maintenance of Rodent Malaria Parasites
Chandra Ramakrishnan, Michael J. Delves, Kalpana Lal, Andrew M. Blagborough, Geoffrey Butcher, Kenneth W. Baker, and Robert E. Sinden
Part II. Genome Manipulation
6. Transfection of Plasmodium falciparum
Melanie Rug and Alexander G. Maier
7. Transfection of Rodent Malaria Parasites
Nisha Philip, Rachael Orr, and Andrew P. Waters
8. Recombination-mediated Genetic Engineering of Plasmodium berghei DNA
Claudia Pfander, Burcu Anar, Mathieu Brochet, Julian C. Rayner, and Oliver Billker
9. Standardization in Generating and Reporting Genetically Modified Rodent Malaria Parasites: The RMgmDB Database
Shahid M. Khan, Hans Kroeze, Blandine Franke-Fayard, and Chris J. Janse
10. Transient Transfection of Plasmodium vivax Blood-stage Parasites
Cecilia P. Sanchez, Judith Pfahler, Hernando A del Portillo, and Michael Lanzer
11. Silencing of Genes and Alleles by RNAi in Anopheles gambiae
Marina Lamacchia, John R. Clayton, Rui Wang-Sattler, Lars M. Steinmetz, Elena A. Levashina, and Stephanie A. Blandin
Part III. 'OMIC' Techniques
12. Analysis of Variant Gene Family Expression by Quantitative PCR
Sohini Sanyal, Thomas J. Templeton, and Cristina K. Moreira
13. DNA Microarray-based Genome-wide Analyses of Plasmodium Parasites
Zbynek Bozdech, Sachel Mok, and Archna P. Gupta
14. Whole-genome Analysis of Plasmodium spp. Utilizing a New Agilent Technologies DNA Microarray Platform
Heather J. Painter, Lindsey M. Altenhofen, Bjoern F.C. Kafsack and Manuel Llinas
15. Transcriptome Analysis using RNA-seq
Wieteke A.M. Hoeijmakers, Richard Bartfai, and Hendrik G. Stunnenberg
16. Experimental Tools for the Study of Protein Phosphorylation in Plasmodium
Dominique Dorin-Semblat, Andrew R. Bottrill, Lev Solyakov, Andrew Tobin, and Christian Doerig
17. Extraction of Hydrophilic Metabolites from Plasmodium falciparum-infected Erythrocytes for Metabolomic Analysis
Kellen L. Olszewski and Manuel Llinas
Part IV. Red Blood Cell Stages
18. Static and Dynamic Imaging of Erythrocyte Invasion and Early Intra-erythrocytic Development in Plasmodium falciparum
David T. Riglar and Jake Baum
19. Flow Cytometry-based Methods for Measurement of Cytosolic Calcium and Surface Protein Expression in Plasmodium falciparum Merozoites
Shailja Singh and Chetan E. Chitnis
20. Microsphiltration: a Microsphere Matrix to Explore Erythrocyte Deformability
Catherine Lavazec, Guillaume Deplaine, Innocent Safeukui, Sylvie Perrot, Genevieve Milon, Odile Mercereau-Puijalon, Peter H. David, and Pierre Buffet
21. Atomic Force Microscopy of Plasmodium-infected Red Blood Cells for Detecting and Localizing Single Molecular Recognition Events
Ang Li, Laurent Renia, Chwee Teck Lim, and Bruce Russell
22. Expressing Full-length Functional PfEMP1 Proteins in the HEK293 Expression System
Anand Srivastava, Yves Durocher, and Benoit Gamain
23. Genome-wide Chromatin Immunoprecipitation-sequencing in Plasmodium
Jose-Juan Lopez-Rubio, T. Nicolai Siegel, and Artur Scherf
24. In situ Fluorescence Visualization of Transcription Sites and Genomic Loci in Blood Stages of Plasmodium falciparum
Liliana Mancio-Silva and Artur Scherf
25. Bioluminescence Imaging of P. berghei Schizont Sequestration in Rodents
Joanna Braks, Elena Aime, Roberta Spaccapelo, Onny Klop, Chris J. Janse, and Blandine Franke-Fayard
Part V. Pre-Erythrocytic Stages
26. Scoring Sporozoite Motility
Allison F. Carey, Robert Menard, and Daniel Y. Bargieri
27. Quantification of Sporozoite Invasion, Migration and Development by Microscopy and Flow Cytometry
Photini Sinnis, Patricia De La Vega, Alida Coppi, Urszula Krzych, and Maria M. Mota
28. Imaging Sporozoite Cell Traversal in the Liver of Mice
Joana Tavares, Pauline Formaglio, Alexander Medvinsky, Robert Menard, and Rogerio Amino
29. Analysis of Liver Stage Development in, and Merozoite Release from Hepatocytes
Rebecca R. Stanway, Jacqueline Schmuckli-Maurer, and Volker T. Heussler
30. Quantitative Analysis of Plasmodium berghei Liver Stages by Bioluminescence Imaging
Takeshi Annoura, Severine Chevalley, Chris J. Janse, Blandine Franke-Fayard, and Shahid M. Khan
Part VI. Immunity to Plasmodium
31. Activation of Human NK Cells by Plasmodium-infected Red Blood Cells
Amir Horowitz and Eleanor M. Riley
32. Chimeric Parasites as Tools to Study Plasmodium Immunology and Assess Malaria Vaccines
Ian Cockburn
33. Development and Use of TCR Transgenic Mice for Malaria Immunology Research
Yun-Chi Chen and Fidel Zavala
34. Tracking the Total CD8 T Cell Response Following Whole Plasmodium Vaccination
Katherine L. Doll, Noah S. Butler, and John T. Harty
Part VII. Controlling Plasmodium Infection
35. Screening Inhibitors of P. berghei Blood Stages using Bioluminescent Reporter Parasites
Jing-wen Lin, Mohammed Sajid, Jai Ramesar, Shahid M. Khan, Chris J. Janse, and Blandine Franke-Fayard
36. Screening and Evaluation of Inhibitors of Plasmodium falciparum Merozoite Egress and Invasion using Cytometry
Anthony Bouillon, Olivier Gorgette, Odile Mercereau-Puijalon, and Jean-Christophe Barale
37. Induction of anti-Plasmodium Immunity Following Sub-patent Infection with Live Erythrocytic Stages and Drug Cure
Danielle I. Stanisic and Michael F. Good
38. Vaccination using Radiation-or Genetically-Attenuated Live Sporozoites
Ashley M Vaughan and Stefan H I Kappe
39. Vaccination using Normal Live Sporozoites Under Drug Treatment
Laurent Renia, Anne Charlotte Gruner, Marjorie Mauduit, and Georges Snounou
40. Assessing Transmission Blockade in Plasmodium spp.
Andrew M. Blagborough, Michael J. Delves, Chandra Ramakrishnan, Kalpana Lal, Geoffrey Butcher, and Robert E. Sinden
41. Mosquito Transgenic Technologies to Reduce Plasmodium Transmission
Silke Fuchs, Tony Nolan, and Andrea Crisanti
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